1. TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage
- Author
-
Luc Negroni, Kapil Gupta, Boris Lenhard, Tao Ye, Stéphane D. Vincent, Petra Hajkova, Nevena Cvetesic, Imre Berger, Changwei Yu, Laszlo Tora, Ferenc Müller, Vincent Hisler, Emese Gazdag, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Imperial College London, University of Bristol [Bristol], Centre for Cold Matter, Blackett Laboratory, Imperial College London, Prince Consort Road, London SW7 2AZ, (CCM), University of Birmingham [Birmingham], Université de Strasbourg (UNISTRA), Commission of the European Communities, and VINCENT, Stéphane
- Subjects
0301 basic medicine ,Transcription, Genetic ,[SDV]Life Sciences [q-bio] ,genetic processes ,General Physics and Astronomy ,RNA polymerase II ,environment and public health ,INITIATION ,Mice ,0302 clinical medicine ,Transcription (biology) ,Promoter Regions, Genetic ,GENE-EXPRESSION ,Multidisciplinary ,biology ,General transcription factor ,LONG TERMINAL REPEATS ,Nuclear Proteins ,TATA Box ,EMBRYONIC-DEVELOPMENT ,Cell biology ,[SDV] Life Sciences [q-bio] ,Multidisciplinary Sciences ,Science & Technology - Other Topics ,Female ,Transcription factor II D ,Transcription ,Transcription factor II A ,Science ,macromolecular substances ,MICE LACKING ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Developmental biology ,Transcription factors ,Animals ,RNA, Messenger ,Transcription factor ,TATA-BINDING PROTEIN ,Science & Technology ,POLYMERASE-II TRANSCRIPTION ,Terminal Repeat Sequences ,Promoter ,General Chemistry ,TBP-LIKE FACTOR ,Mice, Inbred C57BL ,enzymes and coenzymes (carbohydrates) ,030104 developmental biology ,Animals, Newborn ,Gene Expression Regulation ,Transcription Factor TFIIA ,Mutation ,health occupations ,NIH 3T3 Cells ,Oocytes ,biology.protein ,RNA ,FACTOR-2 TRF2 ,Transcription Factor TFIID ,TATA-binding protein ,Transcriptome ,030217 neurology & neurosurgery - Abstract
During oocyte growth, transcription is required to create RNA and protein reserves to achieve maternal competence. During this period, the general transcription factor TATA binding protein (TBP) is replaced by its paralogue, TBPL2 (TBP2 or TRF3), which is essential for RNA polymerase II transcription. We show that in oocytes TBPL2 does not assemble into a canonical TFIID complex. Our transcript analyses demonstrate that TBPL2 mediates transcription of oocyte-expressed genes, including mRNA survey genes, as well as specific endogenous retroviral elements. Transcription start site (TSS) mapping indicates that TBPL2 has a strong preference for TATA-like motif in core promoters driving sharp TSS selection, in contrast with canonical TBP/TFIID-driven TATA-less promoters that have broader TSS architecture. Thus, we show a role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code., The vertebrate TATA-binding protein (TBP) paralogue (TBPL2) is only expressed in growing oocytes, where TBP is absent. Here the authors highlight a unique role for the TBPL2/TFIIA complex in the establishment of the oocyte transcriptome by using a specific TSS recognition code.
- Published
- 2020