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4-Methyl-7-(4-methyl-2-oxo-2H-chromen-6-yl)disulfanyl-2H-chromen-2-one hydrotriiodide was synthesized by the reaction of 7-mercapto-4-methylcoumarin with iodine, and its structure was determined by NMR spectroscopy and X-ray diffraction combined with Raman and IR spectroscopy. The molecular and crystal structure of the reaction product was determined.

The crystal and molecular structure of the reaction product of imidazolidine-2-thione and molecular iodine of the composition (C3H6N2S)2•3I2 was established by X-ray diffraction. The crystal structure is composed of strongly connected five-component associates consisting of three I2 molecules and two heterocyclic molecules. Quantum chemical calculations followed by the topological analysis of the electron density function provided an estimate of the strongest intermolecular interactions in the crystal structure of the molecular adduct.

The p53 gene has been associated with malignant transformation as well as "anti-oncogene" activity. In the present report expression of p53 in resting and activated human blood monocytes and lymphocytes is analyzed. It is found that human monocytes freshly isolated by continuous percoll gradient centrifugation contained detectable level of p53 mRNA. Stimulation of monocytes by potent activation inducer Staphylococcus Aureus Cowan I for 3-5 hr caused disappearance of r53 mRNA. In contrast, induction of high level of TNF-alpha mRNA was detected. Addition of cycloheximide had no effect on p53 mRNA content in stimulated monocytes, and caused disappearance of mRNA in resting cells. In lymphocytes cultures p53 mRNA was absent in freshly isolated cells and in resting lymphocytes cultured for 20 hr. Activation of lymphocytes by lectin caused accumulation of p53 mRNA. We suggest that r53 gene regulation and functions might be different in human monocytes and lymphocytes.

The addition of recombinant tumor necrosis factor or monoclonal antibodies to this factor into human monocyte culture does not change the synthesis of interleukin-8. By contrast, tumor necrosis factor induces the synthesis of interleukin-8 in human lymphocytes.