1. Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes
- Author
-
Kiichi Fukui, Shin'ichiro Kajiyama, Naoko Nose, Takiko Shimada, Shigeki Kawakami, Go Suzuki, Yasuhiko Mukai, Motoyasu Otani, Susumu Uchiyama, Naoki Wada, Daisuke No, and Yukio Akiyama
- Subjects
Chromosomes, Artificial, Bacterial ,DNA, Plant ,Transgene ,Genetic Vectors ,Gene Expression ,Plant Science ,Genetically modified crops ,Genes, Plant ,Poaceae ,Genome ,chemistry.chemical_compound ,Transformation, Genetic ,Aegilops tauschii ,Transgenes ,Gene ,Genetics ,biology ,Gene Transfer Techniques ,food and beverages ,Oryza ,General Medicine ,Plants, Genetically Modified ,biology.organism_classification ,Genetically modified rice ,Microspheres ,Transformation (genetics) ,chemistry ,Cinnamates ,Hygromycin B ,Agronomy and Crop Science ,DNA - Abstract
Transformation with large DNA molecules enables multiple genes to be introduced into plants simultaneously to produce transgenic plants with complex phenotypes. In this study, a large DNA fragment (ca. 100 kb) containing a set of Aegilops tauschii hardness genes was introduced into rice plants using a novel transformation method, called bioactive beads-mediated transformation. Nine transgenic rice plants were obtained and the presence of transgenes in the rice genome was confirmed by PCR and FISH analyses. The results suggested that multiple transgenes were successfully integrated in all transgenic plants. The expression of one of the transgenes, puroindoline b, was confirmed at the mRNA and protein levels in the T(2) generation. Our study clearly demonstrates that the bioactive bead method is capable of producing transgenic rice plants carrying large DNA fragments. This method will facilitate the production of useful transgenic plants by introducing multiple genes simultaneously.
- Published
- 2009
- Full Text
- View/download PDF