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Cerebral ischemia–reperfusion (I/R) injury occurs due to the restoration of blood perfusion after cerebral ischemia, which results in the damage of the brain structures and functions. Unfortunately, currently there are no effective methods for preventing and treating it. The pumilio 2 (PUM2) is a type of RBPs that has been reported to participate in the progression of several diseases. Ferroptosis is reported to be involved in I/R injury. Whether PUM2 modulated I/R injury through regulating ferroptosis remains to be elucidated. The cerebral I/R models including animal middle cerebral artery occlusion/reperfusion (MCAO/R) model and oxygen–glucose deprivation/reperfusion (OGD/R)-induced cortical neuron injury cell model of were established and, respectively. RT-qPCR was applied for evaluating PUM2, SIRT1 and SLC7A11 expression. Western blot was employed for measuring the protein expression levels. The viability of cortical neurons was tested by MTT assay. The histological damage of the brain tissues was assessed by H&E staining. The level of PUM2 was boosted in both the brain tissues of the MCAO model and OGD/R-induced cortical neuron injury model. Silence of PUM2 alleviated MCAO-induced brain injury and decreased the death of PC12 cell exposed to OGD/R. PUM2 also aggravated the accumulation of free iron in MCAO mice and OGD/R-induced cortical neuron injury model. In addition, PUM2 suppressed SLC7A11 via inhibiting expression of SIRT1. Rescue assays unveiled that downregulation of SLC7A11 reversed PUM2 mediated neuroinflammation and brain damage induced by I/R. PUM2 aggravated I/R-induced neuroinflammation and brain damage through the SLC7A11-dependent inhibition of ferroptosis by suppressing SIRT1, highlighting the role of PUM2 in preventing or treating cerebral I/R injury.

Glioblastoma (GBM) is the most common and lethal subtype of glioma, characterized by uncontrolled cancer cell proliferation, extensive infiltration, and therapeutic resistance. Ring finger protein 216 (RNF216) is a RING-type E3 ubiquitin ligase aberrantly expressed in multiple human cancers. Tumor protein 53 (p53) is a transcription factor that acts as a tumor suppressor. This study aimed to compare the RNF216 expression in GBM tissues and normal peritumoral tissues and to examine the effects of RNF216 overexpression/knockdown on tumorigenesis, radioresistance, and the p53 pathway in GBM. The results showed that RNF216 was overexpressed in GBM tissues and cell lines, and high RNF216 expression was related to a poor prognosis. RNF216 overexpression promoted GBM cell growth and inhibited apoptosis, while RNF216 knockdown impaired GBM cell growth and enhanced cell death. RNF216 was also highly expressed in recurrent GBM tissues compared with paired primary tumors. The upregulation of RNF216 not only facilitated GBM cell growth but also protected cells against X-ray irradiation-induced apoptosis and DNA damage, while RNF216 knockdown exerted opposite effects. Moreover, the implantation of GBM cells with RNF216 silencing suppressed tumorigenesis and increased radiosensitivity of mice bearing GBM xenografts. Further analysis revealed that RNF216 overexpression reduced the stability of p53 protein via ubiquitination and negatively regulated the p53 pathway, while RNF216 knockdown preserved the p53 protein. In conclusion, RNF216 effectively attenuated radiation-induced apoptosis and DNA damage in GBM via inducing ubiquitination-mediated degradation of p53. These findings suggest the potential therapeutic use of RNF216 inhibition for tumorigenesis and therapeutic resistance in GBM.

The distributions of thickness of unconsolidated Quaternary sedimentary layers in Jiaozhou Bay and Qingdao offshore area were studied by using 1079-km high-resolution shallow seismic profiles and drilling core data, and the factors controlling the Quaternary evolution were discussed. The results show that such thickness distributions resulted from the coactions of geologic structures and marine hydrodynamic conditions since the Holocene. The geologic structures controlled the slope deposit, proluvial and fluvial fillings since the late Pleistocene. Holocene marine hydrodynamics eroded away sediments at the bay mouth, and tides carried these eroded materials to the sides of the bay mouth and released them there, forming channel-ridge-alternating geomorphic features. During transgressive processes, the sea level rose rapidly, and insufficient sediment supply and tidal actions yielded the relict sediments in the east of Qingdao offshore area.

With available survey data and 237 surface sediment samples, the modern sedimentation in Qingdao bays is studied. The research result shows that the east area is shallower (16.4 m) than that in the west (45.8 m). The geography was formed by a symmetric wave in a sequence of bank — offshore depression — platform — tidal channel — platform — offshore depression — foreshore from south to north. Flood sedimentary systems were formed from inlet of the Jiaozhou Bay in the west to the barrier bar in the east. Lateral sedimentation includes mainly tidal current ridges in two tidal channels. Gravel sandy sediments formed by wave lie in midland of from seashore to wave base. Dynamic functions are mainly tide and wave. Tidal current moves sediment in vertical and horizontal directions, sorting sediments and providing materials for coastwise beaches. The sources of sediment are mainly from eroded headland rocks. In general, grain sizes in this area from the inlet of the Jiaozhou Bay in the west (120°17′) to the barrier bar outside in the east (120°35′) are coarse-fine-coarse, forming gravel-gravelly sand (G-S), sand (S), gravel clay silt (G-YT), clay silt (YT), gravelly silty sand (G-TS), silty sand (TS), gravelly sandy silt (G-ST), and sandy silt (ST) in turn.

Ninety-eight clay mineral samples from the YSDP102 core were analyzed by x-ray diffractometer to study the four clay minerals: illite, chlorite, kaolinite and smectite. Twenty-eight samples had been analyzed on the laser particle-size analyzer to reveal the particle features of the sediments. Distribution of the clay minerals and the particle characteristics in the YSDP102 core show that the core experienced three different depositional periods and formed three different sedimentary intervals due to different sediment sources and different depositional environments. Features of the clay minerals and the heavy minerals in the YSDP102 core indicate that coarse-grained sediments and fine-grained sediments result from different sources. The Yellow Sea Warm Current has greatly influenced the sedimentary framework of this region since the current’s formation.

The sand-hill section in Hongguang, Pengze County, Jiangxi Province is composed of eolian sand beds interbedded with 12 weakly-pedogenetic paleosols, and this sedimentary sequence was formed from about 25 to 13 kaBP. A detailed rock-magnetic study demonstrates that the magnetic minerals in the sequence are dominated by multi-domain magnetite grains in addition to small amounts of maghemite and hematite. Compared with the eolian sand beds, the paleosols are characterized by smaller magnetic particles, higher proportion of low-coercivity minerals and markedly increase in the magnetic mineral content. In the section, various magnetic parameters (K, ARM, SIRM and S ratios) display a roughly gradual increase from eolian sand beds to the overlying paleosols but an abrupt decrease from paleosols to the overlying eolian sand beds. The content of low-coercivity magnetic minerals shows the lowest values in the second eolian sand bed from the bottom of the section, and from the bed upwards it gradually increases on the whole in the eolian sand beds. These magnetic properties reflect regional climatic changes during the last glacial maximum.

Northwest of Cheju Island in the eastern South Yellow Sea there is an arc patch of muddy deposit which coincides with an anticlokwise eddy and thus is referred to as muddy deposit of eddy. Two drilling wells, the YSDP 102 well and the YSDP 103 well, penetrated the muddy deposit and revelated the sedimentary sequence in the sea area during the last deglaciation and the Holocene. Based on shallow seismic profiles, sedimentary features and dating data, stratigraphic units formed in the eastern South Yellow Sea, documents the path, time and process of the earliest post-glacial transgression in the South Yellow Sea are presented, the formation of the Yellow Sea warm current and its control over the Yellow Sea sedimentation are discussed, and deals with the source of the muddy deposit is dealt with.