1. Successful elevation of circulating acetate and propionate by dietary modulation does not alter T-regulatory cell or cytokine profiles in healthy humans: a pilot study
- Author
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Peter R. Gibson, Rosemary A. Ffrench, Jane G. Muir, Menno C. van Zelm, and P. A. Gill
- Subjects
0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,Medicine (miscellaneous) ,030209 endocrinology & metabolism ,Inflammation ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Internal medicine ,T-regulatory cell ,Medicine ,Ingestion ,chemistry.chemical_classification ,030109 nutrition & dietetics ,Nutrition and Dietetics ,medicine.diagnostic_test ,business.industry ,FOXP3 ,Endocrinology ,Cytokine ,chemistry ,Propionate ,medicine.symptom ,business - Abstract
Increased circulating concentrations of short-chain fatty acids (SCFA) achieved by ingestion of high-fibre diets is associated with anti-inflammatory effects through promotion of FoxP3+ regulatory T(reg) cells in mouse models. This study aimed to determine whether similar increments in blood SCFA levels can be achieved in humans and whether these are associated with similar immune modulatory effects. In a pilot single-blinded, randomised, controlled cross-over study in ten healthy subjects, the effects were determined of high- (39 g/day) and low-fibre (18 g/day) intake (all food provided) on SCFA (gas chromatography), proportions of Treg cells (flow cytometry) and a panel of cytokines (multiplex methodology) measured in peripheral blood at day 5 of each diet. Actual fibre intake differed between the diets by 19 [16–21] g/day (P
- Published
- 2019
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