1. Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes
- Author
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R Sh Zakirov, A P Toptygina, K S Kapitanova, and E L Semikina
- Subjects
0301 basic medicine ,Messenger RNA ,Fluorophore ,medicine.diagnostic_test ,Chemistry ,Lymphocyte ,General Medicine ,Molecular biology ,General Biochemistry, Genetics and Molecular Biology ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,medicine ,Transcription factor ,Incubation ,030217 neurology & neurosurgery - Abstract
SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4+ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4+T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.
- Published
- 2019