1. A triple action chemosensor for Cu2+ by chromogenic, Cr3+ by fluorogenic and CN− by relay recognition methods with bio-imaging of HeLa cells
- Author
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Sivan Velmathi, Shu-Pao Wu, Parthiban Venkatesan, Natarajan Vijay, and Gopal Balamurugan
- Subjects
Chromogenic ,Metal ions in aqueous solution ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Fluorescence ,Fluorescence spectroscopy ,0104 chemical sciences ,Rhodamine ,Absorbance ,chemistry.chemical_compound ,chemistry ,Thiophene ,Moiety ,Physical and Theoretical Chemistry ,0210 nano-technology - Abstract
A triple action chemosensor (R1) bearing a rhodamine and thiophene moiety was synthesized by a simple condensation reaction. The sensing behaviour and selectivity of the synthesized chemosensor toward metal ions were studied by UV-Vis and fluorescence spectroscopy. The chemosensor recognized Cu2+ and Cr3+ ions with significant changes in UV-Vis absorbance and fluorescence intensity. The results showed that Cr3+ induced greater fluorescence enhancement whereas Cu2+ ions bound strongly with the receptor by showing a strong absorption band at 554 nm but with weak fluorescence. A visible colour change was observed by the addition of Cu2+ and that colour change is due to the opening of the spirolactam ring triggered by the addition of Cu2+ ions. Job's plot analysis indicated a 1 : 2 and 1 : 1 binding stoichiometry between the chemosensor and Cr3+/Cu2+. Subsequently, the R1 - Cu2+ complex chemosensor was employed to detect CN- in the presence of different anions, such as F-, Cl-, Br-, I-, AcO-, H2PO4-, HSO4-, NO3- and -OH. In addition, the live cell imaging of HeLa cells using R1 and Cr3+ was demonstrated successfully.
- Published
- 2017