1. Sensing by wireless reading Ag/AgCl redox conversion on RFID tag: universal, battery-less biosensor design
- Author
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Nutcha Larpant, Farnoush Faridbod, Wanida Laiwattanapaisal, Javier Sotres, Atefeh Shafaat, Anh Duc Pham, Tautgirdas Ruzgas, Thomas Arnebrant, Mohammad Reza Ganjali, Juan F. Gonzalez-Martinez, and Johan Sjöholm
- Subjects
Materials science ,lcsh:Medicine ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,01 natural sciences ,Horseradish peroxidase ,Redox ,Article ,Silver nanoparticle ,Nanomaterials ,Electron transfer ,Naturvetenskap ,lcsh:Science ,Multidisciplinary ,biology ,Sensors ,lcsh:R ,010401 analytical chemistry ,Substrate (chemistry) ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Multidisciplinary Sciences ,Biosensors ,biology.protein ,lcsh:Q ,Natural Sciences ,0210 nano-technology ,Biosensor - Abstract
Massive integration of biosensors into design of Internet-of-Things (IoT) is vital for progress of healthcare. However, the integration of biosensors is challenging due to limited availability of battery-less biosensor designs. In this work, a combination of nanomaterials for wireless sensing of biological redox reactions is described. The design exploits silver nanoparticles (AgNPs) as part of the RFID tag antenna. We demonstrate that a redox enzyme, particularly, horseradish peroxidase (HRP), can convert AgNPs into AgCl in the presence of its substrate, hydrogen peroxide. This strongly changes the impedance of the tag. The presented example exploits gold nanoparticle (AuNP)-assisted electron transfer (ET) between AgNPs and HRP. We show that AuNP is a vital intermediate for establishing rapid ET between the enzyme and AgNPs. As an example, battery-less biosensor-RFID tag designs for H2O2 and glucose are demonstrated. Similar battery-less sensors can be constructed to sense redox reactions catalysed by other oxidoreductase enzymes, their combinations, bacteria or other biological and even non-biological catalysts. In this work, a fast and general route for converting a high number of redox reaction based sensors into battery-less sensor-RFID tags is described.
- Published
- 2019