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Start Over Author "Nie, Ping" Publisher springer science and business media llc
23 results on '"Nie, Ping"'

14. Panax ginseng-specific sequence characterized amplified region (SCAR) marker for testing medicinal products

Author

Xiao Bingyi, Liu Li, Jie Li, Li Wenli, Qiu-tao Jiang, Ding Ye, Wen-zhang Li, Luo Huiming, and Nie Ping

Subjects
Germplasm, Traditional medicine, Sequence analysis, Metals and Alloys, General Engineering, food and beverages, Amplicon, Biology, biology.organism_classification, complex mixtures, 01 natural sciences, 0104 chemical sciences, RAPD, 010404 medicinal & biomolecular chemistry, 03 medical and health sciences, Ginseng, 0302 clinical medicine, 030220 oncology & carcinogenesis, GenBank, Medicinal herbs, Panax notoginseng
Abstract

To screen genetic polymorphisms of Panax ginseng, as well as those of Panax quinquefolium and Panax notoginseng, analysis of random amplified polymorphic DNA (RAPD) was performed using 120 random primers. Of the successful amplicons obtained, the Panax ginseng-specific RAPD marker C-12 was cloned into a TA vector and sequenced (GenBank access number KU553472). Based on the sequence analysis results, a pair of primers specific to C-12 was designed. Finally, a SCAR marker-based identification system for Panax ginseng was developed after optimization of the reaction conditions. Using this method, two positive bands were stably observed at 300 bp and 130 bp in 33 batches of Panax ginseng samples tested, while negative results were obtained for another 101 batches of samples, including Panax quinquefolium, Panax notoginseng, adulterants, and other medicinal herbs. Thus, we successfully developed a PCR-based method for rapid and effective identification of Panax ginseng, which can be effectively used for the protection and utilization of germplasm resources and identification of the origins of Panax ginseng samples.

Published
2018
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16. Li3V2(PO4)3/nitrogen-doped reduced graphene oxide nanocomposite with enhanced lithium storage properties

Author

Cunliang Zhang, Laifa Shen, Nie Ping, Hongshen Li, Gang Pang, and Xiaogang Zhang

Subjects
Nanocomposite, Materials science, Graphene, Inorganic chemistry, Composite number, Oxide, Nanoparticle, chemistry.chemical_element, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Electrochemistry, 01 natural sciences, Cathode, 0104 chemical sciences, law.invention, chemistry.chemical_compound, chemistry, law, General Materials Science, Lithium, Electrical and Electronic Engineering, 0210 nano-technology
Abstract

The three-dimensional porous Li3V2(PO4)3/nitrogen-doped reduced graphene oxide (LVP/N-RGO) composite was prepared by a facile one-pot hydrothermal method and evaluated as cathode material for lithium-ion batteries. It is clearly seen that the novel porous structure of the as-prepared LVP/N-RGO significantly facilitates electron transfer and lithium-ion diffusion, as well as markedly restrains the agglomeration of Li3V2(PO4)3 (LVP) nanoparticles. The introduction of N atom also has positive influence on the conductivity of RGO, which improves the kinetics of electrochemical reaction during the charge and discharge cycles. It can be found that the resultant LVP/N-RGO composite exhibits superior rate properties (92 mA h g−1 at 30 C) and outstanding cycle performance (122 mA h g−1 after 300 cycles at 5 C), indicating that nitrogen-doped RGO could be used to improve the electrochemical properties of LVP cathodes for high-power lithium-ion battery application.

Published
2016
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