1. Development and characterization of 56 SNP markers in Misgurnus anguillicaudatus
- Author
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Zhigang Liu, Maixin Lu, Guiyun Huang, Fengying Gao, Gang Chen, and Jianmeng Cao
- Subjects
Genetics ,education.field_of_study ,Population ,Single-nucleotide polymorphism ,Misgurnus ,Biology ,biology.organism_classification ,Genetic analysis ,Minor allele frequency ,Loss of heterozygosity ,SNP ,education ,Genotyping ,Ecology, Evolution, Behavior and Systematics - Abstract
The dojo loach Misgurnus anguillicaudatus is an endemic freshwater species in Asia. The effective conservation and molecular-aided selection of M. anguillicaudatus were limited without sufficient molecular markers. In this study, 112 novel single nucleotide polymorphism (SNP) markers were screened based on 2b-RAD sequencing database, and 56 SNPs of them were developed and characterized by genotyping 40 individuals using SNaPshot method. The observed heterozygosity (Ho) ranged from 0.025 to 0.675, while the expected heterozygosity (He) varied from 0.025 to 0.500. The minor allele frequency (MAF) ranged from 0.013 to 0.500. Among these SNPs, 18 loci were found to deviate significantly from the Hardy–Weinberg equilibrium after Bonferroni correction (P
- Published
- 2021