1. Intravenous Followed by X-ray Fused with MRI-Guided Transendocardial Mesenchymal Stem Cell Injection Improves Contractility Reserve in a Swine Model of Myocardial Infarction
- Author
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Jaehyup Kim, Karl K. Vigen, Michael T. Tomkowiak, Denise J. Schwahn, Charles R. Hatt, Peiman Hematti, Eric G. Schmuck, Cathlyn Leitzke, Timothy A. Hacker, Jill M. Koch, Derek J. Hei, Amish N. Raval, Nicholas Hendren, John M. Centanni, Ying qi Zhao, and Zhanhai Li
- Subjects
medicine.medical_specialty ,Swine ,Myocardial Infarction ,Pharmaceutical Science ,Hemodynamics ,Cell Separation ,Mesenchymal Stem Cell Transplantation ,Placebo ,Article ,Injections ,Contractility ,Random Allocation ,Internal medicine ,Genetics ,medicine ,Animals ,Myocardial infarction ,Genetics (clinical) ,Endocardium ,medicine.diagnostic_test ,business.industry ,Mesenchymal stem cell ,Arrhythmias, Cardiac ,Mesenchymal Stem Cells ,Magnetic resonance imaging ,medicine.disease ,Magnetic Resonance Imaging ,Myocardial Contraction ,Injections, Intravenous ,Cardiology ,Molecular Medicine ,Stem cell ,Cardiology and Cardiovascular Medicine ,business - Abstract
The aim of this study is to determine the effects of early intravenous (IV) infusion later followed by transendocardial (TE) injection of allogeneic mesenchymal stem cells (MSCs) following myocardial infarction (MI). Twenty-four swine underwent balloon occlusion reperfusion MI and were randomized into 4 groups: IV MSC (or placebo) infusion (post-MI day 2) and TE MSC (or placebo) injection targeting the infarct border with 2D X-ray fluoroscopy fused to 3D magnetic resonance (XFM) co-registration (post-MI day 14). Continuous ECG recording, MRI, and invasive pressure-volume analyses were performed. IV MSC plus TE MSC treated group was superior to other groups for contractility reserve (p = 0.02) and freedom from VT (p = 0.03) but had more lymphocytic foci localized to the peri-infarct region (p = 0.002). No differences were observed in post-MI remodeling parameters. IV followed by XFM targeted TE MSC therapy improves contractility reserve and suppresses VT but does not affect post-MI remodeling and may cause an immune response.
- Published
- 2015
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