1. Diversity of the exoproteome of Fusarium graminearum grown on plant cell wall
- Author
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Emmanuelle Leize-Wagner, Florence Goubet, Vincent Phalip, François Delalande, Didier Hatsch, Christine Carapito, Paul Dupree, Jean-Marc Jeltsch, Alain Van Dorsselaer, Klotz, Evelyne, Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique, Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Substances naturelles/chimie moléculaire, Université Louis Pasteur - Strasbourg I-Ecole européenne de chimie, polymères et matériaux [Strasbourg]-Centre National de la Recherche Scientifique (CNRS), and Institut Pluridisciplinaire Hubert Curien (IPHC)
- Subjects
Polysaccharides/chemistry ,Humulus lupulus ,MESH: Plants ,MESH: Cellulose 1,4-beta-Cellobiosidase ,Proteomics ,Cellulose 1 ,Plant Proteins/*chemistry/metabolism ,Fusarium ,Cell Wall ,Gene Expression Regulation, Plant ,Glucose/metabolism ,Non-U.S. Gov't ,Plant Proteins ,chemistry.chemical_classification ,MESH: Fusarium ,0303 health sciences ,MESH: Plant Proteins ,biology ,food and beverages ,General Medicine ,Plants ,4-beta-Cellobiosidase/chemistry/metabolism ,MESH: Glucose ,Biochemistry ,Fusarium/*chemistry/growth & development ,Fungus ,Research Support ,Microbiology ,Cell wall ,03 medical and health sciences ,MESH: Cell Wall ,Polysaccharides ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Cellulose 1,4-beta-Cellobiosidase ,Genetics ,Extracellular ,Secretion ,MESH: Gene Expression Regulation, Plant ,030304 developmental biology ,Plants/*microbiology ,030306 microbiology ,Cell Wall/chemistry/*metabolism ,Plant ,biology.organism_classification ,MESH: Polysaccharides ,Glucose ,Enzyme ,Gene Expression Regulation ,chemistry - Abstract
0172-8083 (Print) Journal Article; The exoproteome of the fungus Fusarium graminearum grown on glucose and on hop (Humulus lupulus, L.) cell wall has been investigated. The culture medium was found to contain a higher quantity of proteins and the proteins are more diverse when the fungus is grown on cell wall. Using both 1D and 2D electrophoresis followed by mass spectrometry analysis and protein identification based on similarity searches, 84 unique proteins were identified in the cell wall-grown fungal exoproteome. Many are putatively implicated in carbohydrate metabolism, mainly in cell wall polysaccharide degradation. The predicted carbohydrate-active enzymes fell into 24 different enzymes classes, and up to eight different proteins within a same class are secreted. This indicates that fungal metabolism becomes oriented towards synthesis and secretion of a whole arsenal of enzymes able to digest almost the complete plant cell wall. Cellobiohydrolase is one of the only four proteins found both after growth on glucose and on plant cell wall and we propose that this enzyme could act as a sensor of the extracellular environment. Extensive knowledge of this very diverse F. graminearum exoproteome is an important step towards the full understanding of Fusarium/plants interactions.
- Published
- 2005