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Cupric oxide nanoparticles (CuO NPs) were phytofabricated utilizing leaf extract of Simarouba glauca and aerial extract of Celastrus paniculatus and are considered to hold excellent anticancer capability. Synthesized CuO NPs were characterized for their morphology, crystallinity, and structure. The presence of functional groups of phytochemicals on synthesized nanoparticles was validated by Fourier transform infrared spectroscopy (FTIR) analysis. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) examination reveal the uniform distribution of particles and the average particle size is 35 nm. The anticancer activities on MCF-7 and HT-29 cell lines revealed that CuO NPs synthesized using leaf extract of S. glauca (CuO-SG) induced cell death with half-maximal inhibitory concentration (IC50) value of 107.56 µg/mL and 208.57 µg/mL, while CuO NPs synthesized using the aerial extract of C. paniculatus (CuO-CP) indicated IC50 values of 97.39 µg/mL and 205.11 µg/mL, respectively. To be more precise for anti-cancerous effect, the molecular mechanism was examined in MCF-7 cell line treated with CuO-CP NPs by cell cycle analysis that depicted 75.28% of cell arrest in Sub G0/G1 phase and 71.29% of cells were gated in the late apoptotic phase of Annexin V and propidium iodide (PI) compared to control cells. The present work reports in vivo antitumor studies of CuO-CP NPs against Ehrlich ascites carcinoma (EAC) bearing C57 mice for the first time and was examined by variations in growth parameters, biochemical assays, hematological profile, and histopathological analysis. CuO-CP NPs could eliminate oxidants like lactoperoxidase and myeloperoxidase, stimulate reduced glutathione, restore the hematological profile and increase the life span of tumor-bearing mice treated by them in comparison with control.

In this paper, we give some direct approximation results by modified Kantorovich–Szász type operators involving Charlier polynomials. Further, approximation results are also developed in polynomial weighted spaces. Moreover, for the functions of bounded variation, approximation results are proved. Finally, some graphical examples are provided to show comparisons of convergence between old and modified operators towards a function under different parameters and conditions.

Lamprophyre dykes within the granitoid and charnockite are reported for the first time from the Western Bastar Craton, Chandrapur district, Maharashtra. It shows porphyritic–panidiomorphic texture under a microscope, characterised by the predominance of biotite phenocrysts with less abundance of amphibole and clinopyroxene microphenocryst. The groundmass is composed more of K-feldspars over plagioclase, amphiboles, clinopyroxene, biotite, chlorite, apatite, sphene and magnetite. The mineral chemistry of biotite and magnesio-hornblende is indicative of minette variety of calc-alkaline lamprophyre (CAL), which is further supported by preliminary major oxides and trace element geochemistry. This unique association of CAL with granitoid provides an opportunity to study the spatio-temporal evolution of the lamprophyric magma in relation to the geodynamic perspective of the Bastar Craton.

Objective To determine the ultrasonographic and mammographic features of intraductal breast masses that may be associated with malignancy. Patients and methods We compared US and mammographic findings with the histopathology in 198 patients with 251 intraductal breast masses. The radiologist assessed US features of the mass, its distance from the nipple, the pattern of duct filling by the mass, whether the mass involved the branch ducts and the presence of abnormal axillary lymph nodes. The mammograms were assessed for the presence of a mass, calcifications, a mass with calcifications and asymmetry. Results Histopathology revealed 46 malignant masses (18.3%) and 205 benign masses (81.7%). Malignant masses were larger than benign masses, have greater distance from the nipple, commonly filled the duct completely, extended outside the duct, and involved branch duct whereas benign masses commonly filled the duct incompletely and none extended outside the duct or involved branch duct. On mammography, clustered microcalcifications were commonly associated with malignant masses. Conclusion Intraductal masses completely filled duct, extended outside the duct or involved branch ducts, its distance from the nipple is >15 mm or associated with abnormal axillary lymph nodes on US or microcalcifications on mammography, and all these findings may be associated with malignancy.

Statistical model analysis has been performed for the available neutron multiplicity ( $\nu_{pre}$ data in the literature. Larger $\nu_{pre}$ values for more symmetric reactions have been observed in comparison with asymmetric reactions forming the same compound nucleus, in most cases. A reverse trend has also been noticed in a few cases. A systematic entrance channel dependence of fission timescale is brought out in this work. Fission timescales calculated using the experimental $\nu_{pre}$ values fall into two distinct groups according to the entrance channel mass asymmetry of the reaction with respect to the Businaro-Gallone critical mass asymmetry. The difference in the delay between these two groups ranges between 20 and 100zs, which is larger than that reported in some cases.

We have used the highly conserved heterochromatin component, heterochromatin protein 1 (HP1), as a molecular tag for purifying other protein components of Drosophila heterochromatin. A complex of HP1 associated with the origin recognition complex (ORC) and an HP1/ORC-associated protein (HOAP) was purified from the maternally loaded cytoplasm of early Drosophila embryo. We propose that the DNA-binding activities of ORC and HOAP function to recruit underphosphorylated isoforms of HP1 to sites of heterochromatin nucleation. The roles of highly phosphorylated HP1, other DNA-binding proteins known to interact with HP1, and histone modifying activities in heterochromatin assembly are also addressed.

Quantification of total and individual amino acids in biological fluids such as plasma, urine and cerebrospinal fluid has an important diagnostic implication in laboratory medicine. The present paper describes protocols for the assay of total amino acids by modified method based on dinitrophenyl and HPLC profile involving pre-column derivatization with o-pthalaldehyde (OPA) derivatization, respectively. The method, based on the alkylation of-SH groups prior to OPA derivatization of amino acids followed by reverse phase high performance liquid chromatography, provide a comprehensive profile of more than twenty amino acids (including-SH group containing) in a single run lasting about 45 minutes. The present study, apart from establishing the normal profile of amino acids in plasma of Indian sub population, also presents HPLC profile for some of the rare amino acidopathies.

Quinsy (peritonsillar abscess) is a common emergency seen in otolaryngology practice. These patients are often screened for glandular fever in addition to routine haematological tests. In our unit, we have screened 66 patients with quinsy for glandular fever over a period of 12 months. All these patients were screened for glandular fever by rapid immunoassay. Only one out of 66 patients was tested positive for glandular fever. Due to the extremely low incidence of glandular fever in quinsy patients, we do not see any relevance in screening for glandular fever in quinsy patients. Hence we recommend that routine screening for glandular fever in quinsy patients is an unnecessary invasive investigation for the patients and not cost effective for the hospital.

Fourteen beach samples were collected between Uvary in the northeast and Kanyakumari in the southwest within a coastal stretch of 50 km were studied to confirm the reported occurrence of micro-diamonds in Pulimankulam beach sands. The XRD results suggest that grains with brilliant luster are mainly zircon and the presence of microdiamond could not be ascertained in any of the samples. The fusion of samples with NaOH and further digestion in acid did not leave any residue. This also confirms absence of any non-silicate mineral in the samples.