Search

Tripartite motif protein 21 (TRIM21) is an E3 ubiquitin ligase and cytosolic antibody receptor of the TRIM family. Previous reports have indicated that TRIM21 plays an important role during viral infection. This study aimed at examining the role of TRIM21 in the replication of porcine epidemic diarrhea virus (PEDV) and showed that TRIM21 inhibits PEDV proliferation by targeting and degrading the nucleocapsid (N) protein through the proteasomal pathway. Furthermore, the endogenous expression of TRIM21 was found to be downregulated by PEDV infection in Vero and LLC-PK1 cells. Overexpression of TRIM21 inhibited PEDV replication, whereas knockdown of TRIM21 increased viral titers and N protein levels. TRIM21 was found to interact and colocalize with the N protein, and the TRIM21-mediated antiviral effect was dependent on its ubiquitin ligase activity, which engages in polyubiquitination and degradation of the N protein in a proteasome-dependent manner. Taken together, these findings provide information about the role of TRIM21 in PEDV proliferation and increase our understanding of host-virus interactions. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-021-05080-4.

The widespread changes in forest cover caused by climatological and anthropogenic factors can influence the forest ecosystem and climate system to a great extent. With the increasing availability of remote sensing data, monitoring of forest changes at high temporal resolution and on various scales is becoming more realistic. Though several methods based on time series data have been used to detect forest disturbance, there are few studies paying attention to boreal areas where the forest is significant in regulating the global carbon cycle and biogeophysical processes. In this paper, we present a robust method of Breaks Detection Based On Polynomial Model (BDPM) to track boreal (e.g. Lesser Khingan Mountains) deforestation and forest fires based on the MODIS and Landsat TM time series data. Compared with the previous methods, the BDPM offers the following advantages: (1) Fitting of the polynomial model using the seasonal variation of forests in the whole region instead of a single pixel to avoid error accumulation; (2) to avoid confusion between vegetation change due to climate changes and abrupt forest disturbances, we segmented the long-time NDVI series data into 12 seasonal cycles and simulated the temporal variations in each seasonal cycle.

The snow cover extent in mid-high latitude areas of the Northern Hemisphere has significantly declined corresponding to the global warming, especially since the 1970s. Snow-climate feedbacks play a critical role in regulating the global radiation balance and influencing surface heat flux exchange. However, the degree to which snow cover changes affect the radiation budget and energy balance on a regional scale and the difference between snow-climate and land use/cover change (LUCC)-climate feedbacks have been rarely studied. In this paper, we selected Heilongjiang Basin, where the snow cover has changed obviously, as our study area and used the WRF model to simulate the influences of snow cover changes on the surface radiation budget and heat balance. In the scenario simulation, the localized surface parameter data improved the accuracy by 10 % compared with the control group. The spatial and temporal analysis of the surface variables showed that the net surface radiation, sensible heat flux, Bowen ratio, temperature and percentage of snow cover were negatively correlated and that the ground heat flux and latent heat flux were positively correlated with the percentage of snow cover. The spatial analysis also showed that a significant relationship existed between the surface variables and land cover types, which was not obviously as that for snow cover changes. Finally, six typical study areas were selected to quantitatively analyse the influence of land cover types beneath the snow cover on heat absorption and transfer, which showed that when the land was snow covered, the conversion of forest to farmland can dramatically influence the net radiation and other surface variables, whereas the snow-free land showed significantly reduced influence. Furthermore, compared with typical land cover changes, e.g., the conversion of forest into farmland, the influence of snow cover changes on net radiation and sensible heat flux were 60 % higher than that of land cover changes, indicating the importance of snow cover changes in the surface-atmospheric feedback system.

The Northeast China is one of typical regions experiencing intensive human activities within short time worldwide. Particularly, as the significant changes of agriculture land and forest, typical characteristics of pattern and process of agroforestry ecotone change formed in recent decades. The intensive land use change of agroforestry ecotone has made significant change for regional land cover, which had significant impact on the regional climate system elements and the interactions among them. This paper took agroforestry ecotone of Nenjiang River Basin in China as study region and simulated temperature change based on land cover change from 1950s to 1978 and from 1978 to 2010. The analysis of temperature difference sensitivity to land cover change based on Weather Research and Forecasting (WRF) model showed that the land cover change from 1950s to 1978 induced warming effect over all the study area, including the change of grassland to agriculture land, grassland to deciduous broad-leaved forest, and deciduous broad-leaved forest to shrub land. The land cover change from 1978 to 2010 induced cooling effect over all the study area, including the change of deciduous broad-leaved forest to agriculture land, grassland to agriculture land, shrub land to agriculture land, and deciduous broad-leaved forest to grassland. In addition, the warming and cooling effect of land cover change was more significant in the region scale than specific land cover change area.

Global warming has contributed to the extension of the growing season in North Hemisphere. In this paper, we investigated the spatial characteristics of the date of the start of the season (SOS), the date of the end of the season (EOS) and the length of the season (LOS) and their change trends from 1982 to 2015 in Northeast China. Our results showed that there was a significant advance of SOS and a significant delay of EOS, especially in the north part of Northeast China. For the average change slope of EOS in the study area, the delay trend was 0.25 d/y, which was more obvious than the advance trend of −0.13 d/y from the SOS. In particular, the LOS of deciduous needleleaf forest (DNF) and grassland increased with a trend of 0.63 d/y and 0.66 d/y from 1982 to 2015, indicating the growth season increased 21.42 and 22.44 days in a 34-year period, respectively. However, few negative signals were detected nearby Hulun Lake, suggesting that the continuous climate warming in the future may bring no longer growing periods for the grass in the semiarid areas as the drought caused by climate warming may limit the vegetation growth.

Land cover change, as one of the most important driving forces to climate change, has become the research focus of the global environmental change research and global land project. More researchers studied on the global influence of Land-Use and Land-Cover Change and proved that land use change occurred at different temperature zones may produce different climate effects. For example, deforestation in tropical areas would lead to higher temperatures as the decreasing of evapotranspiration caused by the reduction of roughness and the decreasing of drag coefficient and leaf area index while, in boreal areas, similar deforestation would cause lower temperature as the increasing of albedo particularly during winter with the snow cover. However, the impact of deforestation in the temperate regions on the climate still existed uncertainty and the impacts of deforestation at different humidity conditions on climate has not explored yet. From this perspective, this article used Weather Research and Forecasting model to simulate the impact of deforestation on the temperature of Northeastern China. In this study, we designed two scenarios in July and December, respectively: One was simulated without human intervention, and the second one was simulated with the current forest covers. The results showed that the temperature in both summer and winter showed a decreasing trend when the conversion of forest to farmland occurred in northeastern China. In order to further explore the humidity impacts on the temperature, we performed sample analysis on humid, sub-humid, and semi-arid regions. According to the results, the maximum variation of temperature was found in humid areas, especially in December when the temperature decreased around 4–5 °C, while the change in semi-arid and sub-humid areas is relatively small.

SAMHD1 is a type I interferon (IFN) inducible host innate immunity restriction factor that inhibits an early step of the viral life cycle. The underlying mechanisms of SAMHD1 transcriptional regulation remains elusive. Here, we report that inducing SAMHD1 upregulation is part of an early intrinsic immune response via TLR3 and RIG-I/MDA5 agonists that ultimately induce the nuclear translocation of the interferon regulation factor 3 (IRF3) protein. Further studies show that IRF3 plays a major role in upregulating endogenous SAMHD1 expression in a mechanism that is independent of the classical IFN-induced JAK-STAT pathway. Both overexpression and activation of IRF3 enhanced the SAMHD1 promoter luciferase activity, and activated IRF3 was necessary for upregulating SAMHD1 expression in a type I IFN cascade. We also show that the SAMHD1 promoter is a direct target of IRF3 and an IRF3 binding site is sufficient to render this promoter responsive to stimulation. Collectively, these findings indicate that upregulation of endogenous SAMHD1 expression is attributed to the phosphorylation and nuclear translocation of IRF3 and we suggest that type I IFN induction and induced SAMHD1 expression are coordinated.