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Your search keyword '"Li, Shuangjing"' showing total 8 results
Start Over Author "Li, Shuangjing" Publisher springer science and business media llc
8 results on '"Li, Shuangjing"'

3. Intravenous infusion of human umbilical cord Wharton’s jelly-derived mesenchymal stem cells as a potential treatment for patients with COVID-19 pneumonia

Author

Zhang, Yingxin, primary, Ding, Jie, additional, Ren, Shaoda, additional, Wang, Weihua, additional, Yang, Yapei, additional, Li, Shuangjing, additional, Meng, Min, additional, Wu, Tiejun, additional, Liu, Daliang, additional, Tian, Suochen, additional, Tian, Hui, additional, Chen, Shuangfeng, additional, and Zhou, Changhui, additional

Published
2020
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4. Disulfide-stabilized diabody antiCD19/antiCD3 exceeds its parental antibody in tumor-targeting activity

Author

Xiong Dongsheng, Shi Rui-zan, Li Shuangjing, Wang Jianxiang, Yan Yan, Jiang Linlin, Yan Cihui, Wang Min, Li Wei, Fan Dongmei, and Yang Ming

Subjects
Cancer Research, Lymphoma, B-Cell, CD3 Complex, T cell, Antigens, CD19, Antibody Affinity, Mice, Nude, CD19, Mice, Antigen, In vivo, Antibodies, Bispecific, medicine, Animals, Humans, B cell, Mice, Inbred BALB C, biology, Chemistry, General Medicine, Flow Cytometry, Xenograft Model Antitumor Assays, Molecular biology, Raji cell, medicine.anatomical_structure, Oncology, Polyclonal antibodies, biology.protein, Molecular Medicine, Immunotherapy, Antibody, Genetic Engineering
Abstract

A diabody is a bispecific antibody that is capable of recruiting a polyclonal T cell to antibody target-expressing tumor cells. However, the two chains of diabodies tend to dissociate because they are integrated non-covalently. Therefore, it is necessary to remodel the diabody to increase its stability in order to enhance the antitumor activity. We constructed an antiCD3×antiCD19 diabody with one binding site for the T cell antigen receptor (TCRCD3) and the other for the B cell-specific antigen (CD19) by recombinant gene engineering technology. Cysteine residues were introduced into the V domains of the anti-CD3 segment. The stability and cytotoxicity of the two diabodies were compared in vitro and vivo. The disulfide-stabilized (ds) diabodies produced by Escherichia coli were secreted with high yields in a fully active form without a decrease in affinity. Compared with the parental diabody, the disulfide-stabilized (ds) diabody proved more stable in vitro and in vivo without reducing binding affinity. Both were able to effectively eliminate human lymphoma Raji cells by redirecting T lymphocytes in vitro and in vivo, but the ds diabody was more effective in inhibiting the growth of xenografts transplanted in BALB/C nude mice. The antiCD3×antiCD19 ds diabody is more suitable for a controlled polyclonal T cell therapy of human CD19-positive B cell malignancies than its parental diabody.

Published
2012

5. FTY720 inhibits mesothelioma growth in vitro and in a syngeneic mouse model

Author

Szymiczek, Agata, primary, Pastorino, Sandra, additional, Larson, David, additional, Tanji, Mika, additional, Pellegrini, Laura, additional, Xue, Jiaming, additional, Li, Shuangjing, additional, Giorgi, Carlotta, additional, Pinton, Paolo, additional, Takinishi, Yasutaka, additional, Pass, Harvey I., additional, Furuya, Hideki, additional, Gaudino, Giovanni, additional, Napolitano, Andrea, additional, Carbone, Michele, additional, and Yang, Haining, additional

Published
2017
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