1. Imaging the cellular uptake of tiopronin-modified gold nanoparticles
- Author
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Xiaoqing Cai, S.-Y. Chen, Hsiang-Hsin Chen, Yeukuang Hwu, Ivan M. Kempson, Yi-Yun Chen, Giorgio Margaritondo, Sheng-Feng Lai, Cheng-Liang Wang, Chia-Chi Chien, Chung-Shi Yang, Cai, Xiaoqing, Chen, Hsiang-Hsin, Wang, Cheng-Liang, Chen, Shin-Tai, Lai, Sheng-Feng, Chien, Chia-Chi, Chen, Yi-Yun, Kempson, Ivan M, Hwu, Yeukuang, Yang, C S, and Margaritondo, G
- Subjects
Resolution (mass spectrometry) ,Biocompatibility ,Cell Survival ,Reducing agent ,Cells ,Analytical chemistry ,Metal Nanoparticles ,Biochemistry ,Mass Spectrometry ,Analytical Chemistry ,Microscopy, Electron, Transmission ,Microscopy ,medicine ,Humans ,Inductively coupled plasma mass spectrometry ,Cells, Cultured ,Chemistry ,X-Rays ,Tiopronin ,Endocytosis ,Radiography ,Transmission electron microscopy ,Colloidal gold ,Gold ,Synchrotrons ,medicine.drug ,Nuclear chemistry - Abstract
Well-dispersed gold nanoparticles (NP) coated with tiopronin were synthesized by X-ray irradiation without reducing agents. High-resolution transmission electron microscopy shows that the average core diameters of the NPs can be systematically controlled by adjusting the tiopronin to Au mole ratio in the reaction. Three methods were used to study the NP uptake by cells: quantitative measurements by inductively coupled plasma mass spectrometry, direct imaging with high lateral resolution transmission electron microscopy and transmission X-ray microscopy. The results confirmed that the NP internalization mostly occurred via endocytosis and concerned the cytoplasm. The particles, in spite of their small sizes, were not found to arrive inside the cell nuclei. The synthesis without reducing agents and solvents increased the biocompatibility as required for potential applications in analysis and biomedicine in general Refereed/Peer-reviewed
- Published
- 2011