163 results on '"LO GIUDICE, A"'
Search Results
2. Tauro-Urso-Deoxycholic Acid Trials in Amyotrophic Lateral Sclerosis: What is Achieved and What to Expect
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Lo Giudice, Maria, primary, Cocco, Antoniangela, additional, Reggiardo, Giorgio, additional, Lalli, Stefania, additional, and Albanese, Alberto, additional
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- 2023
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3. Glass beads from a Scythian grave on the island of Khortytsia (Zaporizhzhia, Ukraine): insights into bead making through 3D imaging
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Nykonenko, Dmytro, primary, Yatsuk, Oleh, additional, Guidorzi, Laura, additional, Lo Giudice, Alessandro, additional, Tansella, Francesca, additional, Cesareo, Ludovica Pia, additional, Sorrentino, Giusi, additional, Davit, Patrizia, additional, Gulmini, Monica, additional, and Re, Alessandro, additional
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- 2023
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4. Dysregulation of testis mRNA expression levels in hatchery-produced vs wild greater amberjack Seriola dumerili
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Lavecchia, Anna, primary, Manzari, Caterina, additional, Pousis, Chrysovalentinos, additional, Mansi, Luigi, additional, Cox, Sharon N., additional, Mylonas, Constantinos C., additional, Zupa, Rosa, additional, Lo Giudice, Claudio, additional, De Virgilio, Caterina, additional, Picardi, Ernesto, additional, Ventriglia, Gianluca, additional, Pesole, Graziano, additional, and Corriero, Aldo, additional
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- 2023
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5. Growth differentiation factor-15 and circulating biomarkers as predictors of periodontal treatment effects in patients with periodontitis: a randomized-controlled clinical trial
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Isola, Gaetano, primary, Tartaglia, Gianluca Martino, additional, Santonocito, Simona, additional, Chaurasia, Akhilanand, additional, Marya, Anand, additional, and Lo Giudice, Antonino, additional
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- 2023
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6. External root resorption and rapid maxillary expansion in the short-term: a CBCT comparative study between tooth-borne and bone-borne appliances, using 3D imaging digital technology
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Leonardi, Rosalia, primary, Ronsivalle, Vincenzo, additional, Isola, Gaetano, additional, Cicciù, Marco, additional, Lagravère, Manuel, additional, Flores-Mir, Carlos, additional, and Lo Giudice, Antonino, additional
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- 2023
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7. Analysing medical predictors for the outcome of infertility treatment: a 5-year follow-up survey
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Stein, Lea Joana, primary, Rösner, Sabine, additional, Lo Giudice, Alessandra, additional, Ditzen, Beate, additional, and Wischmann, Tewes, additional
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- 2023
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8. An archaeometric contribution to the interpretation of blue-green glass beads from Iron age Central Italy
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Yatsuk, Oleh, primary, Koch, Leonie, additional, Gorghinian, Astrik, additional, Fiocco, Giacomo, additional, Davit, Patrizia, additional, Giannossa, Lorena Carla, additional, Mangone, Annarosa, additional, Francone, Serena, additional, Serges, Alessandra, additional, Re, Alessandro, additional, Lo Giudice, Alessandro, additional, Ferretti, Marco, additional, Malagodi, Marco, additional, Iaia, Cristiano, additional, and Gulmini, Monica, additional
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- 2023
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9. Tooth automatic segmentation from CBCT images: a systematic review
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Polizzi, Alessandro, primary, Quinzi, Vincenzo, additional, Ronsivalle, Vincenzo, additional, Venezia, Pietro, additional, Santonocito, Simona, additional, Lo Giudice, Antonino, additional, Leonardi, Rosalia, additional, and Isola, Gaetano, additional
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- 2023
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10. Assessment of the Efficacy of Cryolipolysis on Abdominal Fat Deposits: A Prospective Study
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Coiante, Edoardo, primary, Pensato, Rosita, additional, Hadji, Ilyes, additional, Lo Giudice, Giorgio, additional, Pizza, Chiara, additional, SidAhmed-Mezi, Mounia, additional, D’Andrea, Francesco, additional, Meningaud, Jean Paul, additional, Hersant, Barbara, additional, and La Padula, Simone, additional
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- 2023
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11. An archaeometric contribution to the interpretation of blue-green glass beads from Iron age Central Italy
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Oleh Yatsuk, Leonie Koch, Astrik Gorghinian, Giacomo Fiocco, Patrizia Davit, Lorena Carla Giannossa, Annarosa Mangone, Serena Francone, Alessandra Serges, Alessandro Re, Alessandro Lo Giudice, Marco Ferretti, Marco Malagodi, Cristiano Iaia, and Monica Gulmini
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Archeology ,Chemistry (miscellaneous) ,Materials Science (miscellaneous) ,Conservation ,Spectroscopy ,Computer Science Applications - Abstract
Several types of (mostly) blue-green glass beads from Iron-Age archaeological sites in Central Italy were studied using a range of spectroscopic techniques: portable X-Ray Fluorescence spectrometry, Fibre Optics Reflectance Spectroscopy, Scanning Electron Microscopy coupled with Energy Dispersive X-ray Spectrometry, micro-Raman spectroscopy and Laser Ablation Inductively Coupled Plasma Mass Spectrometry. Complementary information was gathered from each technique and discussed in the frame of the archaeological typology of the objects. The systematic evaluation of the results allowed us to draw some conclusions on the raw materials employed for primary production and to highlight some provenance indicators in the glass. Some of the beads found in the Iron Age (IA) contexts were preliminarily attributed to the Final Bronze Age (FBA) production based on their typology, and the compositional data obtained in this work confirmed that they were low magnesium high potassium (LMHK) glass, typical of FBA in the Italian peninsula. Other beads were assigned to low magnesium glass (LMG) or high magnesium glass (HMG), thus giving further information on the fluxing agents employed in the Early Iron Age (EIA) and beyond. Colour variations among the beads reflected their chemical composition, with different bead typologies coloured in a specific way. In some instances, it was possible to establish different origins for the colouring raw materials. The provenance of the samples was difficult to place, but the chemical evidence suggested a subdivision within the raw glass used to produce the beads: for one set of samples, a local origin of the glass could be hypothesised, whereas several production sites in the Near East were suggested for most of the beads considered in this study. Some preliminary clues for the local working of imported glass were also highlighted for one typological group.
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- 2023
12. Assessment of the Efficacy of Cryolipolysis on Abdominal Fat Deposits: A Prospective Study
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Edoardo Coiante, Rosita Pensato, Ilyes Hadji, Giorgio Lo Giudice, Chiara Pizza, Mounia SidAhmed-Mezi, Francesco D’Andrea, Jean Paul Meningaud, Barbara Hersant, and Simone La Padula
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Surgery - Published
- 2023
13. Micro-PIXE and micro-IBIL characterization of lapis lazuli samples from Myanmar mines and implications for provenance study
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Guidorzi, Laura, primary, Re, Alessandro, additional, Magalini, Marta, additional, Angelici, Debora, additional, Borghi, Alessandro, additional, Vaggelli, Gloria, additional, Fantino, Fulvio, additional, Rigato, Valentino, additional, La Torre, Leonardo, additional, Lemasson, Quentin, additional, Pacheco, Claire, additional, Pichon, Laurent, additional, Moignard, Brice, additional, and Lo Giudice, Alessandro, additional
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- 2023
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14. Palatal changes after treatment of functional posterior cross-bite using elastodontic appliances: a 3D imaging study using deviation analysis and surface-to-surface matching technique
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Lo Giudice, Antonino, primary, Ronsivalle, Vincenzo, additional, Conforte, Cristina, additional, Marzo, Giuseppe, additional, Lucchese, Alessandra, additional, Leonardi, Rosalia, additional, and Isola, Gaetano, additional
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- 2023
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15. A possible unique ecosystem in the endoglacial hypersaline brines in Antarctica
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Guglielmin, M., primary, Azzaro, M., additional, Buzzini, P., additional, Battistel, D., additional, Roman, M., additional, Ponti, S., additional, Turchetti, B., additional, Sannino, C., additional, Borruso, L., additional, Papale, M., additional, and Lo Giudice, A., additional
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- 2023
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16. Micro-PIXE and micro-IBIL characterization of lapis lazuli samples from Myanmar mines and implications for provenance study
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Laura Guidorzi, Alessandro Re, Marta Magalini, Debora Angelici, Alessandro Borghi, Gloria Vaggelli, Fulvio Fantino, Valentino Rigato, Leonardo La Torre, Quentin Lemasson, Claire Pacheco, Laurent Pichon, Brice Moignard, and Alessandro Lo Giudice
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Fluid Flow and Transfer Processes ,IBIL ,lapis lazuli, provenance, ion beam analysis, PIXE, IBIL ,provenance ,lapis lazuli ,PIXE ,General Physics and Astronomy ,ion beam analysis - Abstract
The provenance of raw lapis lazuli used since the Neolithic Age for the realization of carved artefacts can be pivoting for the reconstruction of ancient trade routes. The role of main provider of this material in ancient times is generally attributed to the Badakhshan quarries (Afghanistan), although other deposits could have been exploited as well since antiquity. The systematic multi-technique analysis of lapis lazuli rocks coming from four known source areas (located in present-day Afghanistan, Tajikistan, Siberia and Chile) has led in the last years to the compilation of a protocol for provenance determination. The protocol is based on differences in the physical–chemical properties measured with non-invasive techniques (Ion Beam Analysis (IBA)), making it suitable also for precious ancient artefacts. In this work, 10 new reference samples from the Mogok quarry (Myanmar), previously subjected to a complete petrographic and mineralogical characterization, have been analysed employing $$\mu$$ μ -PIXE (Proton Induced X-ray Emission) and $$\mu$$ μ -IBIL (Ion Beam Induced Luminescence) techniques. The subdivision of Myanmar samples into three groups, previously suggested, is corroborated by diopside trace elements concentrations. The results also allow to include Myanmar as a new provenance in the analytical protocol, indicating that provenance markers such as presence/absence of wollastonite, altered pyrites and Sr content are still valid to discriminate also the Myanmar lapis lazuli from Chilean or Siberian ones. New weaker markers (Zn content in diopside, Se and Cu contents in pyrite) are proposed for the discrimination of Myanmar from Afghan or Tajik provenances; however, from the analysis it arose the need of investigating new mineral phases with IBA to find strong markers for a definitive discrimination with samples from Southwest Asia.
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- 2023
17. Assessment of the accuracy of imaging software for 3D rendering of the upper airway, usable in orthodontic and craniofacial clinical settings
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Antonino Lo Giudice, Vincenzo Ronsivalle, Giorgio Gastaldi, Rosalia Leonardi, Lo Giudice, A., Ronsivalle, V., Gastaldi, G., and Leonardi, R.
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Imaging, Three-Dimensional ,Upper airway ,Cone-beam computed tomography ,Humans ,Reproducibility of Results ,OSAS ,Orthodontics ,Nose ,3D rendering ,Software - Abstract
Background Several semi-automatic software are available for the three-dimensional reconstruction of the airway from DICOM files. The aim of this study was to evaluate the accuracy of the segmentation of the upper airway testing four free source and one commercially available semi-automatic software. A total of 20 cone-beam computed tomography (CBCT) were selected to perform semi-automatic segmentation of the upper airway. The software tested were Invesalius, ITK-Snap, Dolphin 3D, 3D Slicer and Seg3D. The same upper airway models were manually segmented (Mimics software) and set as the gold standard (GS) reference of the investigation. A specific 3D imaging technology was used to perform the superimposition between the upper airway model obtained with semi-automatic software and the GS model, and to perform the surface-to-surface matching analysis. The accuracy of semi-automatic segmentation was evaluated calculating the volumetric mean differences (mean bias and limits of agreement) and the percentage of matching of the upper airway models compared to the manual segmentation (GS). Qualitative assessments were performed using color-coded maps. All data were statistically analyzed for software comparisons. Results Statistically significant differences were found in the volumetric dimensions of the upper airway models and in the matching percentage among the tested software (p 3; matching = 90.05%) followed by ITK-Snap (mean bias = − 2.52 cm3; matching = 84.44%), Seg 3D (mean bias = 3.21 cm3, matching = 87.36%), 3D Slicer (mean bias = − 4.77 cm3; matching = 82.08%) and Dolphin 3D (difference mean = − 6.06 cm3; matching = 78.26%). According to the color-coded map, the dis-matched area was mainly located at the most anterior nasal region of the airway. Volumetric data showed excellent inter-software reliability (GS vs semi-automatic software), with coefficient values ranging from 0.904 to 0.993, confirming proportional equivalence with manual segmentation. Conclusion Despite the excellent inter-software reliability, different semi-automatic segmentation algorithms could generate different patterns of inaccuracy error (underestimation/overestimation) of the upper airway models. Thus, is unreasonable to expect volumetric agreement among different software packages for the 3D rendering of the upper airway anatomy.
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- 2022
18. Towards a universal flu vaccine
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Lo Giudice, Cristina, primary
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- 2022
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19. A biopolymer to fight pancreatic cancer
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Lo Giudice, Cristina, primary
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- 2022
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20. External root resorption (ERR) and rapid maxillary expansion (RME) at post-retention stage: a comparison between tooth-borne and bone-borne RME
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Leonardi, Rosalia, primary, Ronsivalle, Vincenzo, additional, Barbato, Ersilia, additional, Lagravère, Manuel, additional, Flores-Mir, Carlos, additional, and Lo Giudice, Antonino, additional
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- 2022
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21. The organic residues of lining in transport vessels from the Red Sea coast of Eritrea: a further element to understand past commercial relations
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Zerai, Abraham, primary, Guerrini, Camilla, additional, Łucejko, Jeannette J., additional, Ribechini, Erika, additional, Piccirillo, Anna, additional, Massa, Serena, additional, Mandelli, Chiara, additional, Medin, Tsegai, additional, Re, Alessandro, additional, Lo Giudice, Alessandro, additional, Davit, Patrizia, additional, and Gulmini, Monica, additional
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- 2022
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22. The organic residues of lining in transport vessels from the Red Sea coast of Eritrea: a further element to understand past commercial relations
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Abraham Zerai, Camilla Guerrini, Jeannette J. Łucejko, Erika Ribechini, Anna Piccirillo, Serena Massa, Chiara Mandelli, Tsegai Medin, Alessandro Re, Alessandro Lo Giudice, Patrizia Davit, and Monica Gulmini
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Archeology ,Late antiquity ,Materials Science (miscellaneous) ,Conservation ,Torpedo Jars ,Archaeometry ,Computer Science Applications ,FT-IR ,Adulis ,Indian Ocean Trade ,GC-MS ,Bitumen ,Lining ,Chemistry (miscellaneous) ,Spectroscopy - Abstract
The archaeological site of Adulis lays on the Red Sea Coast of Eritrea and during Late antiquity played a significant role in interregional commerce among the Mediterranean, the Red Sea and the Indian Ocean coasts. Contacts with the Eastern Mediterranean, Arabian Peninsula and the Sasanian world have been attested from different classes of pottery that were brought to light from on-going excavations at the site. Transport vessels have attracted particular attention as they testify the extent of trades and exchange networks. Transport vessels were coated by organic materials to seal porosity and make them suitable to transport different liquids and/or food. The characterisation of coating materials helped shedding light on their function, and support the attribution to different classes of transport vessels found in the Indian Ocean and Red Sea worlds. Here, the characterisation of the organic lining detected on a set of samples identified as Late Roman Amphora 1 is discussed. Results from the chemical analyses, performed preliminarily by FT-IR and then by GC–MS, revealed that bitumen was used for lining the jars, thus leading to set the classification of the amphorae within the wide class of the so- called Torpedo jars. By overcoming the question of typological complexity posed from macroscopic examination of the sherds, the chemical investigation contributed here crucial information for the interpretation of past trading in the Indian Ocean. The research gave clues to broaden the distribution of the Torpedo jars to Adulis, giving an unexpected insight into the trading routes of the past.
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- 2022
23. Oncological outcomes of cribriform histology pattern in prostate cancer patients: a systematic review and meta-analysis
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Giorgio Ivan, Russo, Timo, Soeterik, Ignacio, Puche-Sanz, Giuseppe, Broggi, Arturo, Lo Giudice, Cosimo, De Nunzio, Riccardo, Lombardo, Giancarlo, Marra, and Giorgio, Gandaglia
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cribriform ,pattern ,prostate ,Cancer Research ,Oncology ,Urology - Abstract
Changes applied to the Prostate cancer (PCa) histopathology grading, where patients with cribriform patterns (CP) may be categorized as grade group 2 and could hypothetically be surveilled. However, CP has been associated with worse oncological outcomes. The aim of our study is to systematically review and meta-analyze the available evidence on CP in PCa patients.This analysis was registered on PROSPERO (CRD42022298473). We performed a systematic literature search of PubMed, EMBASE and Scopus using Medical Subject Headings (MeSH) indexes, keyword searches, and publication types until December 2021. The search terms included: "prostate", "prostate cancer" and "cribriform". We also searched reference lists of relevant articles. Eligible studies included published journal articles that provided quantitative data on the association between cribriform patterns at radical prostatectomy and the presence of extra-prostatic extension (EPE), seminal vesicle invasion (SVI), positive surgical margins (PSM), biochemical recurrence (BCR) or cancer specific mortality (CSM).Overall, 31 studies were included for the quantitative analysis. All articles have been published during a span of 11 years (2011-2022) with a mean month of follow-up of 62.87 months. The mean quality of these studies, assessed with the Newcastle Ottawa Scale was 6.27. We demonstrated that CP was associated with greater risk of EPE (odds ratio [OR] 1.96; P 0.0001), SVI (OR: 2.89; p 0.01), and PSM (OR: 1.88; p 0.0007). Our analyses showed that CP was associated with greater risk of BCR (hazard ratio [HR]: 2.14; p 0.01) and of CSM (HR: 3.30, p 0.01).The presence of CP is associated with adverse pathology at radical prostatectomy and worse biochemical recurrence and cancer specific mortality. These results highlight the importance of a better pathologic report of CP to advise clinician for a strict follow-up in PCa patients.
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- 2022
24. PD-1, PD-L1 and cAMP immunohistochemical expressions are associated with worse oncological outcome in patients with bladder cancer
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Giorgio Ivan Russo, Nicolò Musso, Arturo Lo Giudice, Maria Giovanna Asmundo, Marina Di Mauro, Paolo G. Bonacci, Mariacristina Massimino, Dalida Bivona, Stefania Stefani, Elisabetta Pricoco, Matteo Ferro, Massimo Camarda, Sebastiano Cimino, Giuseppe Morgia, Rosario Caltabiano, and Giuseppe Broggi
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Cancer Research ,Genes ,Oncology ,NGS ,Bladder cancer ,PD-1 ,Immunotherapy ,General Medicine ,Tumor-infiltrating lymphocytes - Abstract
Purpose In this study, we aimed to identify prognostic factors of cancer mortality in patients who received radical cystectomy and to identify genomic alterations in a sub-cohort of patients with locally advanced (pT3-4) and/or positive lymph nodes bladder cancer (BC). Methods We collected 101 BC samples from 2010 to 2018 who previously received radical cystectomy. Immunohistochemical slides were evaluated for PPAR, cAMP, IMP3, Ki67, CDK4, POU5F1, Cyclin E and MDM2, p65, CD3, CD4, CD8, CD20, CD68, CD163, FOXP3, PD-1 and PD-L1 expression. We calculated a prognostic score (PS) based on the positivity to PD-1, PD-L1 and of cAMP (final score ranging from 0 to 3). DNA of each sample have been used for sequencing by NGS in a sub-cohort of 6 patients with locally advanced (pT3-4) and/or positive lymph nodes BC. Results PD-1 + (HR [hazard ratio] 2.59; p = 0.04), PD-L1+ (HR = 6.46; p + (HR 3.04; p = 0.02) were independent predictors of cancer-specific mortality (CSM). Increase of PS (score = 0 as reference) was associated with CSM, 0.81 (p = 0.80), 4.72 (p = 0.01) and 10.51 (p Conclusion BC exhibited heterogenous protein expression and variable genomic features. Identification of expression of PD-1, PD-L1 and cAMP could help in predicting oncological outcomes.
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- 2022
25. Characterization of the exopolymer-producing Pseudoalteromonas sp. S8-8 from Antarctic sediment
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Rizzo, Carmen, primary, Perrin, Elena, additional, Poli, Annarita, additional, Finore, Ilaria, additional, Fani, Renato, additional, and Lo Giudice, Angelina, additional
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- 2022
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26. PD-1, PD-L1 and cAMP immunohistochemical expressions are associated with worse oncological outcome in patients with bladder cancer
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Russo, Giorgio Ivan, primary, Musso, Nicolò, additional, Lo Giudice, Arturo, additional, Asmundo, Maria Giovanna, additional, Di Mauro, Marina, additional, Bonacci, Paolo G., additional, Massimino, Mariacristina, additional, Bivona, Dalida, additional, Stefani, Stefania, additional, Pricoco, Elisabetta, additional, Ferro, Matteo, additional, Camarda, Massimo, additional, Cimino, Sebastiano, additional, Morgia, Giuseppe, additional, Caltabiano, Rosario, additional, and Broggi, Giuseppe, additional
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- 2022
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27. Evaluation of mandibular changes after rapid maxillary expansion: a CBCT study in youngsters with unilateral posterior crossbite using a surface-to-surface matching technique
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Vincenzo D'Antò, Khaled Aboulazm, Antonino Lo Giudice, Vincenzo Ronsivalle, Rosalia Leonardi, Gaetano Isola, Manuel O. Lagravère, Leonardi, Rosalia Maria, Aboulazm, Khaled, Lo Giudice, Antonino, Ronsivalle, Vincenzo, D'Anto, Vincenzo, Lagravere, Manuel, and Isola, Gaetano
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Palatal Expansion Technique ,Hyrax ,Orthodontics ,Mandible ,Posterior crossbite ,Mandibular asymmetry ,Surface matching ,03 medical and health sciences ,0302 clinical medicine ,Maxilla ,medicine ,Orthodontic ,Humans ,Crossbite ,Rapid maxillary expansion ,Facial asymmetry ,General Dentistry ,biology ,business.industry ,RME ,CBCT ,Spiral Cone-Beam Computed Tomography ,030206 dentistry ,Cone-Beam Computed Tomography ,biology.organism_classification ,medicine.disease ,Morphological asymmetry ,030220 oncology & carcinogenesis ,business ,Malocclusion ,Human ,Facial symmetry - Abstract
Objectives: The purpose of this study was to evaluate mandibular asymmetry in youngsters with posterior unilateral crossbite (PUXB), through cone-beam computed tomography and reverse engineering technique, before and after rapid maxillary expansion (RME) treatment. Materials and methods: Forty cone-beam computed tomography (CBCT) images were obtained from all patients at two time points, namely T0 acquired before the placement of a Hyrax expander and T1 after appliance removal. The CBCT scans were segmented and volume rendered into a surface there-dimensional (3D) mesh model. Thereafter, mandibular models were digitally registered by using a “best-fit” algorithm. Surface and volumetric changes, between T0 and T1, were compared by using Student’s t tests. Results: A slight increase of 0.45 cm3 of the total mandibular volume was found at T1 when compared with T0 (p < 0.001). The mandibular hemi-volume on the crossbite side (CB) was slightly smaller than the non-crossbite side both at T0 and T1. However, the mean differences of hemi-volume from the CB (crossbite) and non-CB side between T0 and T1 show a decrease of 0.26 cm3 (p < 0.001). Findings for the surface-to-surface deviation analysis demonstrated a fine percentage of matching at T0 which slightly improved at T1 (p < 0.001). Conclusions: Youngsters affected by PUXB showed a very slight and not statistically significant volumetric and morphological asymmetry between CB side and non-CB side at T0. However, the change in mean differences of 0.26 cm cannot be considered clinically relevant. Clinical relevance: Mandibles in young PUXB patients exhibit only a very mild mandibular asymmetry. Although the statistically significant mean change found right after RME removal cannot be considered clinically relevant, a more consistent sample and a longer follow-up could be of interest in explaining the short-term findings.
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- 2020
28. Evaluation of the accuracy of orthodontic models prototyped with entry-level LCD-based 3D printers: a study using surface-based superimposition and deviation analysis
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Vincenzo Ronsivalle, Giuseppe Palazzo, Lorenzo Rustico, Antonino Lo Giudice, Kaled Aboulazm, and Gaetano Isola
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Surface (mathematics) ,Computer science ,3D printing ,Orthodontics ,CAD-CAM ,law.invention ,Root mean square ,03 medical and health sciences ,0302 clinical medicine ,Digital file ,law ,Digital orthodontics ,Superimposition ,General Dentistry ,Simulation ,Liquid-crystal display ,business.industry ,Dental Models ,030206 dentistry ,Prototyping ,Models, Dental ,Liquid Crystals ,Digital dentistry ,030220 oncology & carcinogenesis ,Printing, Three-Dimensional ,Computer-Aided Design ,business - Abstract
The objective of this study was to evaluate the accuracy of dental models prototyped via entry-level liquid crystal display (LCD) 3D printers.Identical prototyped models were generated from a master digital file testing two entry-level LCD-based 3D printers and using one professional-grade 3D printer as gold standard (GS), with 50-µm and 100-µm layer thickness. Each 3D-printed model was scanned, and a specific 3D technology was used to perform surface-based superimposition and deviation analysis to evaluate trueness and precision. The distances between surface points of two superimposed models were converted to root mean square (RMS) and statistically analyzed.The RMS values detected were significantly higher in dental models prototyped with entry-level compared to the SLA printer (p 0.001), in terms of trueness (50 µm: GS 0.075 mm, LCD1 0.192 mm, LCD2 0.179 mm; 100 µm: GS 0.066 mm, LCD1 0.209 mm, LCD2 0.199 mm) and precision (50 µm: GS 0.028 mm, LCD1 0.075 mm, LCD2 0.085 mm; 100 µm: GS 0.039 mm, LCD1 0.096 mm, LCD2 0.101 mm). No significant differences were found between the values of RMS of both entry-level 3D printers (p 0.05). Layer thickness did not affect either the trueness or precision of the 3D-printed models (p 0.05).Entry-level LCD-based 3D printers are not as accurate as professional-grade 3D printer, but still close to orthodontics clinical threshold values.Clinicians should evaluate the intended application of 3D-printed orthodontic models before using entry-level 3D printers.
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- 2021
29. Assessment of the accuracy of imaging software for 3D rendering of the upper airway, usable in orthodontic and craniofacial clinical settings
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Lo Giudice, Antonino, primary, Ronsivalle, Vincenzo, additional, Gastaldi, Giorgio, additional, and Leonardi, Rosalia, additional
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- 2022
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30. Antarctic Porifera homogenates as a source of enzymes and antibacterial substances: first results
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Caruso, Gabriella, primary, Papale, Maria, additional, Azzaro, Maurizio, additional, Rizzo, Carmen, additional, Laganà, Pasqualina, additional, Caruso, Rosalba, additional, and Lo Giudice, Angelina, additional
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- 2022
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31. Fragility fractures in psoriatic arthritis patients: a matched retrospective cohort study
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Enrique R. Soriano, John F. Jaramillo Gallego, Luciano F. Lo Giudice, Marina Scolnik, Florencia S. Pierini, and Nicolas M. Marin Zucaro
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medicine.medical_specialty ,Osteoporosis ,Cohort Studies ,03 medical and health sciences ,Psoriatic arthritis ,0302 clinical medicine ,Rheumatology ,Internal medicine ,Statistical significance ,medicine ,Humans ,030212 general & internal medicine ,Retrospective Studies ,030203 arthritis & rheumatology ,Proportional hazards model ,business.industry ,Incidence ,Incidence (epidemiology) ,Arthritis, Psoriatic ,Retrospective cohort study ,General Medicine ,medicine.disease ,Cohort ,Spinal Fractures ,Female ,business ,Osteoporotic Fractures - Abstract
To compare incidental fragility fractures in psoriatic arthritis (PsA) patients with matched controls from a university hospital.Consecutive PsA patients were matched (age and sex) with controls (1:2). Follow-up began at index date, defined as the date of PsA diagnosis for cases and their respective controls, until the last hospital visit, death or the end of the study (31 December 2017). Electronic medical records were reviewed for osteoporotic fractures. Incidence rates per 100,000 persons-years (PY) of distinct types of fractures after index dates were calculated and compared between groups. A multivariate Cox regression analysis was performed to investigate determinants of fractures.Ninety-two PsA patients and 184 controls were included. No difference was found in the overall fracture incidence rate per 100,000 PY between PsA and controls (1020 95% CI 510-1930, vs 870 95% CI 520-1390, p = 0.36). Vertebral fractures were numerically more frequent in PsA patients with an incidence rate of 1020 (95% CI 510-1930) per 100,000 PY versus 460 (95% CI 240-920), per 100,000 PY in the control group but it did not reach statistical significance (p = 0.06). In the Cox regression analysis, after adjusting for bisphosphonate use, only age (HR 1.10, 1.05-1.16, p 0.001) and female sex (HR 3.94, 1.11-13.91, p = 0.03) were associated with fractures while PsA diagnosis and use of glucocorticoids were not.In this cohort of PsA patients, no overall increased risk of fractures was found in comparison with matched controls. Key Points • PsA could have different effects on bone, leading to confusing results in bone densitometry readings contributing to the difficulty in establishing the real prevalence of OP in PsA. • Vertebral fractures were more frequent in PsA patients compared to controls, but it did not reach statistical significance. No difference was found in the overall fracture incidence rate.
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- 2020
32. An Approach to Extracting Topic-guided Views from the Sources of a Data Lake
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Domenico Potena, Domenico Ursino, Paolo Lo Giudice, Claudia Diamantini, and Emanuele Storti
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Information retrieval ,Computer Networks and Communications ,Computer science ,05 social sciences ,Unstructured data ,02 engineering and technology ,Structuring ,Data warehouse ,Theoretical Computer Science ,Metadata ,Thematic map ,Knowledge extraction ,020204 information systems ,0502 economics and business ,Similarity (psychology) ,Metadata management ,0202 electrical engineering, electronic engineering, information engineering ,050211 marketing ,Software ,Information Systems - Abstract
In the last years, data lakes are emerging as an effective and an efficient support for information and knowledge extraction from a huge amount of highly heterogeneous and quickly changing data sources. Data lake management requires the definition of new techniques, very different from the ones adopted for data warehouses in the past. In this scenario, one of the most challenging issues to address consists in the extraction of topic-guided (i.e., thematic) views from the (very heterogeneous and often unstructured) sources of a data lake. In this paper, we propose a new network-based model to uniformly represent structured, semi-structured and unstructured sources of a data lake. Then, we present a new approach to, at least partially, “structuring” unstructured data. Finally, we define a technique to extract topic-guided views from the sources of a data lake, based on similarity and other semantic relationships among source metadata.
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- 2020
33. Peculiarities of extracellular polymeric substances produced by Antarctic bacteria and their possible applications
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Ilaria Finore, Angelina Lo Giudice, Carmen Rizzo, and Annarita Poli
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Geologic Sediments ,Antarctic Regions ,Context (language use) ,Applied Microbiology and Biotechnology ,Astrobiology ,03 medical and health sciences ,Extracellular polymeric substance ,Antarctic matrices ,psychrophiles ,Ice Cover ,Seawater ,Psychrophile ,biotechnological value ,Ecosystem ,Phylogeny ,030304 developmental biology ,Parageobacillus thermantarcticus ,0303 health sciences ,Bacteria ,biology ,Extracellular Polymeric Substance Matrix ,030306 microbiology ,Chemistry ,Thermophile ,Temperature ,General Medicine ,biology.organism_classification ,EPS purification ,thermophiles ,Biotechnology - Abstract
Extracellular polymeric substances (EPSs) possess diversified ecological role, including the cell adhesion to surfaces and cell protection, and are highly involved in the interactions between the bacterial cells and the bulk environments. Interestingly, EPSs find valuable applications in the industrial field, due to their chemical versatility. In this context, Antarctic bacteria have not been given the attention they deserve as producers of EPS molecules and a very limited insight into their EPS production capabilities and biotechnological potential is available in literature to date. Antarctic EPS-producing bacteria are mainly psychrophiles deriving from the marine environments (generally sea-ice and seawater) around the continent, whereas a unique thermophilic bacterium, namely Parageobacillus thermantarcticus strain M1, was isolated from geothermal soil of the crater of Mount Melbourne. This mini-review is aimed at showcasing the current knowledge on EPS-producing Antarctic bacteria and the chemical peculiarities of produced EPSs, highlighting their biotechnological potential and the yet unexplored treasure they represent for biodiscovery.
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- 2020
34. Towards a universal flu vaccine
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Cristina, Lo Giudice
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,General Chemistry ,Condensed Matter Physics - Published
- 2022
35. A biopolymer to fight pancreatic cancer
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Cristina, Lo Giudice
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,General Chemistry ,Condensed Matter Physics - Published
- 2022
36. Hyaluronidase injection vs direct surgery in surgical rhinoplasty patients treated with non-surgical nasal reshaping with hyaluronic acid filler: a systematic review
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Rauso, Raffaele, primary, Fragola, Romolo, additional, Nicoletti, Giovanni Francesco, additional, Zerbinati, Nicola, additional, Cirillo, Pierfrancesco, additional, Bertossi, Dario, additional, Finocchi, Valerio, additional, Pascali, Michele, additional, Bove, Pierfrancesco, additional, and Lo Giudice, Giorgio, additional
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- 2021
- Full Text
- View/download PDF
37. Author Correction: Reovirus directly engages integrin to recruit clathrin for entry into host cells
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Terence S. Dermody, Simon J. L. Petitjean, David Alsteens, Pavithra Aravamudhan, Melanie Koehler, Xayathed Somoulay, Mégane A. Poncin, Andra C. Dumitru, Cristina Lo Giudice, and Jinsung Yang
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Multidisciplinary ,Science ,Integrin ,biology.protein ,General Physics and Astronomy ,General Chemistry ,Biology ,Entry into host ,Clathrin ,General Biochemistry, Genetics and Molecular Biology ,Cell biology - Published
- 2021
38. Hyaluronic acid injections in post-enucleation or evisceration socket syndrome: a case series
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Migliardi, Renata, primary, Modugno, Alessandra, additional, Chirico, Fabrizio, additional, Zerbinati, Nicola, additional, Nicoletti, Giovanni Francesco, additional, Lo Giudice, Giorgio, additional, and Rauso, Raffaele, additional
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- 2021
- Full Text
- View/download PDF
39. Metal Resistance in Bacteria from Contaminated Arctic Sediment is Driven by Metal Local Inputs
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Stefania Giannarelli, Simona Caputo, Marco Graziano, Luigi Michaud, Maria Papale, Emilio De Domenico, Federica Moscheo, Paul Eric Aspholm, Stefano Miserocchi, Massimo Onor, Angelina Lo Giudice, Antonella Conte, Carmen Rizzo, and Maurizio Azzaro
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Geologic Sediments ,010504 meteorology & atmospheric sciences ,Health, Toxicology and Mutagenesis ,Sewage ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Bioremediation ,Arctic River ,Rivers ,Drug Resistance, Bacterial ,medicine ,Ecotoxicology ,anthropogenic input ,heavy metals ,Phylogeny ,0105 earth and related environmental sciences ,Bacteria ,biology ,Arctic Regions ,Norway ,business.industry ,Sediment ,General Medicine ,Seasonality ,Contamination ,medicine.disease ,biology.organism_classification ,Pollution ,sediment ,Arctic ,Metals ,Environmental chemistry ,Environmental science ,Seasons ,Stenotrophomonas ,business ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Anthropogenic impact over the Pasvik River (Arctic Norway) is mainly caused by emissions from runoff from smelter and mine wastes, as well as by domestic sewage from the Russian, Norwegian, and Finnish settlements situated on its catchment area. In this study, sediment samples from sites within the Pasvik River area with different histories of metal input were analyzed for metal contamination and occurrence of metal-resistant bacteria in late spring and summer of 2014. The major differences in microbial and chemical parameters were mostly dependent on local inputs than seasonality. Higher concentrations of metals were generally detected in July rather than May, with inner stations that became particularly enriched in Cr, Ni, Cu, and Zn, but without significant differences. Bacterial resistance to metals, which resulted from viable counts on amended agar plates, was in the order Ni2+> Pb2+>Co2+>Zn2+>Cu2+>Cd2+>Hg2+, with higher values that were generally determined at inner stations. Among a total of 286 bacterial isolates (mainly achieved from Ni- and Pb-amended plates), the 7.2% showed multiresistance at increasing metal concentration (up to 10,000 ppm). Selected multiresistant isolates belonged to the genera Stenotrophomonas, Arthrobacter, and Serratia. Results highlighted that bacteria, rapidly responding to changing conditions, could be considered as true indicators of the harmful effect caused by contaminants on human health and environment and suggested their potential application in bioremediation processes of metal-polluted cold sites.
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- 2019
40. A multipurpose X-ray fluorescence scanner developed for in situ analysis
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F. Arneodo, Fernando Marte, A. Lo Giudice, L. Palla, F. Castella, Caroline Czelusniak, Francesco Taccetti, V. Conicella, Rodrigo Torres, A. Mazzinghi, Noemi Elisa Mastrangelo, Lisa Castelli, Alessandro Re, Lorenzo Giuntini, N. Gelli, C. Ruberto, Damasia Gallegos, A. Di Giovanni, C. Censori, D. Zafiropulos, and Marcos Tascon
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Engineering drawing ,Scanner ,Computer science ,XRF SCANNER ,Permission ,XRF IMAGING ,Open system (systems theory) ,Field (computer science) ,purl.org/becyt/ford/1 [https] ,Software portability ,Software ,purl.org/becyt/ford/1.4 [https] ,TRANSPORTABLE INSTRUMENT ,XRF scanner ,General Environmental Science ,In situ analysis ,ACQUISITION ,business.industry ,IN SITU ANALYSIS ,Transportable instrument ,Acquisition ,Cultural heritage ,XRF imaging ,General Earth and Planetary Sciences ,CULTURAL HERITAGE ,General Agricultural and Biological Sciences ,business - Abstract
Over the time, instrument transportability has become more and more important, especially in Cultural Heritage, as often artworks cannot be moved from their site, either because of the size or due to problems with permission issues, or simply because moving them to a laboratory is physically impossible, as e.g. in the case of mural paintings. For this reason, the INFN-CHNet, the network for Cultural Heritage studies of the Italian National Institute of Nuclear Physics (INFN), has developed an XRF scanner for in situ analyses. The instrument is the result of a wide collaboration, where different units of the network have been developing the diverse parts, then merged in a single system. The XRF scanner has been designed to be a four-season and green instrument. The control/acquisition/analysis software has been fully developed by our group, using only open-source software. Other strong points of the system are easiness of use, high portability, good performances and ultra-low radiation dispersion, which allows us to use even when the public can be present. It can run both with mains or on batteries, in the latter case with a maximum runtime longer than 10 h. It has a very low cost, when compared to commercial systems with equivalent performances, and easily replaceable components, which makes it accessible for a much wider portion of the interested community. The system has been thought and designed as an open system, suitable for further development/improvements, that can result interesting for non-conventional XRF analysis. The CHNet XRF scanner has proved to be really very well suited for applications in the Cultural Heritage field, as testified by the many recent applications. This paper describes the present version of our instrument and reports on the tests performed to characterise its main features. Fil: Taccetti, F.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Castelli, L.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Czelusniak, C.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Gelli, N.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Mazzinghi, A.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Palla, L.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Ruberto, C.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Censori, C.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Lo Giudice, A.. Università di Torino; Italia. Istituto Nazionale di Fisica Nucleare; Italia Fil: Re, A.. Università di Torino; Italia. Istituto Nazionale di Fisica Nucleare; Italia Fil: Zafiropulos, D.. Istituto Nazionale di Fisica Nucleare; Italia Fil: Arneodo, F.. New York University Abu Dhab; Emiratos Arabes Unidos Fil: Conicella, V.. New York University Abu Dhab; Emiratos Arabes Unidos Fil: Di Giovanni, A.. New York University Abu Dhab; Emiratos Arabes Unidos Fil: Torres, R.. New York University Abu Dhab; Emiratos Arabes Unidos Fil: Castella, F.. Universidad Nacional de San Martín. Instituto de Investigaciones sobre el Patrimonio Cultural; Argentina Fil: Mastrangelo, Noemi Elisa. Universidad Nacional de San Martín. Instituto de Investigaciones sobre el Patrimonio Cultural; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gallegos, D.. Universidad Nacional de San Martín. Instituto de Investigaciones sobre el Patrimonio Cultural; Argentina Fil: Tascon, Marcos. Universidad Nacional de San Martín. Instituto de Investigaciones sobre el Patrimonio Cultural; Argentina. Universidad Nacional de San Martín. Instituto de Investigación e Ingeniería Ambiental. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigación e Ingeniería Ambiental; Argentina Fil: Marte, F.. Universidad Nacional de San Martín. Instituto de Investigaciones sobre el Patrimonio Cultural; Argentina Fil: Giuntini, L.. Università degli Studi di Firenze; Italia
- Published
- 2019
41. Evaluation of the accuracy of orthodontic models prototyped with entry-level LCD-based 3D printers: a study using surface-based superimposition and deviation analysis
- Author
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Lo Giudice, Antonino, primary, Ronsivalle, Vincenzo, additional, Rustico, Lorenzo, additional, Aboulazm, Kaled, additional, Isola, Gaetano, additional, and Palazzo, Giuseppe, additional
- Published
- 2021
- Full Text
- View/download PDF
42. Reovirus directly engages integrin to recruit clathrin for entry into host cells
- Author
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Koehler, Melanie, primary, Petitjean, Simon J. L., additional, Yang, Jinsung, additional, Aravamudhan, Pavithra, additional, Somoulay, Xayathed, additional, Lo Giudice, Cristina, additional, Poncin, Mégane A., additional, Dumitru, Andra C., additional, Dermody, Terence S., additional, and Alsteens, David, additional
- Published
- 2021
- Full Text
- View/download PDF
43. Interventional sialendoscopy in parotidomegaly related to eating disorders
- Author
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Colella, Giuseppe, primary, Lo Giudice, Giorgio, additional, De Luca, Roberto, additional, Troiano, Antonio, additional, Lo Faro, Carmelo, additional, Santillo, Vincenzo, additional, and Tartaro, Gianpaolo, additional
- Published
- 2021
- Full Text
- View/download PDF
44. Evaluation of the changes of orbital cavity volume and shape after tooth-borne and bone-borne rapid maxillary expansion (RME)
- Author
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Cristina Grippaudo, Vincenzo Ronsivalle, Antonino Lo Giudice, Lorenzo Rustico, Manuel O. Lagravère, and Carmelo Nicotra
- Subjects
Palatal Expansion Technique ,Cone beam computed tomography ,Adolescent ,lcsh:Specialties of internal medicine ,Settore MED/28 - MALATTIE ODONTOSTOMATOLOGICHE ,03 medical and health sciences ,0302 clinical medicine ,lcsh:RC581-951 ,Maxilla ,Humans ,Medicine ,Rapid maxillary expansion ,In patient ,Child ,General Dentistry ,Orthodontics ,business.industry ,Research ,CBCT ,030206 dentistry ,bone-borne expander ,Cone-Beam Computed Tomography ,tooth-borne expander ,medicine.anatomical_structure ,Otorhinolaryngology ,rapid maxillary expansion ,Orbital cavity ,orthodontic treatment ,sense organs ,Neurology (clinical) ,orthodontics ,business ,Orbit ,Tooth ,030217 neurology & neurosurgery ,Volume (compression) ,Orbit (anatomy) - Abstract
Objective To assess and compare volumetric and shape changes of the orbital cavity in patients treated with tooth-borne (TB) and bone-borne (BB) rapid maxillary expansion (RME). Study design Forty adolescents with bilateral maxillary cross-bite received tooth-borne (TB group = 20; mean age 14.27 ± 1.36 years) or bone-borne (BB group = 20; mean age of 14.62 ± 1.45 years) maxillary expander. Cone-beam computed tomography (CBCT) were taken before treatment (T1) and 6-month after the expander activation (T2). Volumetric and shape changes of orbital cavities were detected by referring to a specific 3D digital technology involving deviation analysis of T1/T2 CBCT-derived models of pulp chamber. Student’s t tests were used to 1) compare T1 and T2 volumes of orbital cavities in TB and BB groups, 2) compare volumetric changes and the percentage of matching of 3D orbital models (T1-T2) between the two groups. Results Both TB and BB groups showed a slight increase of the orbital volume (0.64 cm3 and 0.77 cm3) (p p p > 0.05). The areas of greater changes were detected in the proximity of the frontozygomatic and frontomaxillary sutures. Conclusion TB-RME and BB-RME would not seem to considerably affect the anatomy or the volume of the orbital cavity in adolescents.
- Published
- 2020
45. The step further smile virtual planning: milled versus prototyped mock-ups for the evaluation of the designed smile characteristics
- Author
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Alessandra Lucchese, Luca Ortensi, Gaetano Isola, Erica Lo Castro, Marco Farronato, and Antonino Lo Giudice
- Subjects
Adult ,Clinical tests ,Matching (statistics) ,Digital analysis ,Esthetics, Dental ,Smiling ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,Stl file ,Smile virtual planning ,General Dentistry ,Simulation ,Milled mock-up ,business.industry ,Contrast (statistics) ,Prototyped mock-up ,Mock ups ,030229 sport sciences ,030206 dentistry ,Smile aesthetics ,lcsh:RK1-715 ,Smile virtual planning, Digital dentistry, Smile aesthetics, Prototyped mock-up, Milled mock-up ,Digital dentistry ,Virtual planning ,lcsh:Dentistry ,Printing, Three-Dimensional ,Computer-Aided Design ,business ,Research Article - Abstract
Background Mock-up based approach allows the preview of the aesthetic rehabilitation, however, it is crucial that the mock-up does not differ from the expected aesthetic outcomes. With CAD-CAM technologies, it is possible to directly create mock-ups from virtual planned smile project, with greater accuracy and efficiency compared to the conventional moulded mock-ups. In this study, we investigated the trueness of mock-ups obtained with milling and 3D printing technology and a full digital work-flow system. Methods Ten adults subjects were included and digital smile design/digital wax-up were performed to enhance the aesthetic of maxillary anterior region. Ten milled mock-ups and 10 prototyped mock-ups were obtained from the original .stl file and a digital analysis of trueness was carried out by superimposing the scanned-milled mock-ups and the scanned-prototyped mock-ups to the digital wax-up, according to the surface-to-surface matching technique. Specific linear measurements were performed to investigate and compare the dimensional characteristics of the physical manufactures, the 3D project and the scanned mock-ups. All data were statistically analyzed. A clinical test was also performed to assess the fitting of the final manufacture. Results The prototyped mock-ups showed a significant increment of the transversal measurements (p p p Conclusion Both prototype and milled mock-ups showed a slight dimensional increment comparing to the original 3D project, with milled-mock-ups showing less fitting after clinical tests. Caution must be taken when assessing the trueness of scanned manufacture since an intrinsic error in the system can underestimate the dimensions of the real object.
- Published
- 2020
46. Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166
- Author
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Pierre van der Bruggen, Camille Lemaigre, Cesar Augusto Valades-Cruz, Henri-François Renard, François Tyckaert, Ludger Johannes, Ruddy Wattiez, Cristina Lo Giudice, Pierre Morsomme, David Alsteens, Massiullah Shafaq-Zadah, Christian Wunder, Thibault Hirsch, UCL - SST/LIBST - Louvain Institute of Biomolecular Science and Technology, Bodescot, Myriam, Assimilation de Données et Microscopie à Feuille de Lumière Structurée pour la Modélisation des Voies d'Endocytose et d'Exocytose en Cellule Unique - - DALLISH2016 - ANR-16-CE23-0005 - AAPG2016 - VALID, Développment d'une infrastructure française distribuée coordonnée - - France-BioImaging2010 - ANR-10-INBS-0004 - INBS - VALID, Initiative d'excellence - Paris Sciences et Lettres - - PSL2010 - ANR-10-IDEX-0001 - IDEX - VALID, Endocytic Membrane Compartmentalization by Galectins - GALECTCOMPART - - EC:FP7:ERC2014-04-01 - 2019-03-31 - 340485 - VALID, Université Catholique de Louvain = Catholic University of Louvain (UCL), Space-timE RePresentation, Imaging and cellular dynamics of molecular COmplexes (SERPICO), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Chimie biologique des membranes et ciblage thérapeutique (CBMCT - UMR 3666 / U1143), Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Mons (UMons), Walloon Excellence in Life sciences and BIOtechnology [Liège] (WELBIO), This work was supported by grants from the 'Fonds National de la Recherche Scientifique' (FNRS, CDR-J.0119.19) and the 'Communauté française de Belgique–Actions de Recherches Concertées' (17/22-085). This work was also supported by the French National Research Agency (DALLISH–ANR-16-CE23-0005), and by Inria in the frame of NAVISCOPE-IPL (Inria Project Lab). The bioprofiling platform used for the proteomic analysis was supported by the FNRS, the European Regional Development Fund, and the Walloon Region, Belgium. The LLSM was financed by PIA France-Bioimaging (ANR-10-INBS-04_01), LabEx DCBiol, LabEx CelTisPhyBio ANR-11-LABX-0038, Agence Nationale de la Recherche (ANR-16-CE23-0005-02, ANR-19-CE13-0001-01), HFSP (RGP0029/2014), and European Research Council (ERC project 340485). We greatly acknowledge the Cell and Tissue Imaging Facility (PICT-IBiSA) and Nikon Imaging Centre, Institut Curie, member of the French National Research Infrastructure France-BioImaging (ANR-10-INBS-04). H.-F.R. is a FNRS postdoctoral research fellow (Belgium). F.T., T.H. and C.L. are supported by PhD fellowships from FRIA/FNRS (Belgium). C.L.G. is an EMBO Long-term postdoctoral fellow. P.V.D.B. and D.A. are supported by WELBIO (Fédération Wallonie-Bruxelles, Belgium). This work was supported by the European Research Council under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 758224). D.A. is a research associate of the FNRS (Belgium)., ANR-16-CE23-0005,DALLISH,Assimilation de Données et Microscopie à Feuille de Lumière Structurée pour la Modélisation des Voies d'Endocytose et d'Exocytose en Cellule Unique(2016), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), European Project: 340485,EC:FP7:ERC,ERC-2013-ADG,GALECTCOMPART(2014), UCL - SSS/DDUV - Institut de Duve, and Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)
- Subjects
Fetal Proteins ,0301 basic medicine ,Intravital Microscopy ,Endocytic cycle ,General Physics and Astronomy ,Mice ,0302 clinical medicine ,Cell Movement ,Neoplasms ,Chlorocebus aethiops ,RNA, Small Interfering ,lcsh:Science ,Multidisciplinary ,Cell adhesion molecule ,Chemistry ,Activated-Leukocyte Cell Adhesion Molecule ,Signal transducing adaptor protein ,Endocytosis ,Recombinant Proteins ,3. Good health ,Cell biology ,Galectin-8 ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Science ,Cell Adhesion Molecules, Neuronal ,Galectins ,[SDV.CAN]Life Sciences [q-bio]/Cancer ,Genetics and Molecular Biology ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,[SDV.CAN] Life Sciences [q-bio]/Cancer ,Antigens, CD ,Cell Line, Tumor ,Cell Adhesion ,Animals ,Humans ,Cell adhesion ,[SDV.BC] Life Sciences [q-bio]/Cellular Biology ,ALCAM ,Adaptor Proteins, Signal Transducing ,Cell Membrane ,General Chemistry ,Fibroblasts ,Clathrin ,030104 developmental biology ,General Biochemistry ,lcsh:Q - Abstract
While several clathrin-independent endocytic processes have been described so far, their biological relevance often remains elusive, especially in pathophysiological contexts such as cancer. In this study, we find that the tumor marker CD166/ALCAM (Activated Leukocyte Cell Adhesion Molecule) is a clathrin-independent cargo. We show that endophilin-A3—but neither A1 nor A2 isoforms—functionally associates with CD166-containing early endocytic carriers and physically interacts with the cargo. Our data further demonstrates that the three endophilin-A isoforms control the uptake of distinct subsets of cargoes. In addition, we provide strong evidence that the construction of endocytic sites from which CD166 is taken up in an endophilin-A3-dependent manner is driven by extracellular galectin-8. Taken together, our data reveal the existence of a previously uncharacterized clathrin-independent endocytic modality, that modulates the abundance of CD166 at the cell surface, and regulates adhesive and migratory properties of cancer cells., How and which cell surface molecules are taken up by clathrin-independent endocytosis is an ongoing area of research. Here, the authors show that the tumor marker CD166 is a clathrin-independent cargo that is taken up by endophilin-A3 and galectin-8, which regulates cancer cell migration.
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- 2020
47. First evidence of quorum sensing activity in bacteria associated with Antarctic sponges
- Author
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Luigi Michaud, A. Lo Giudice, Consolazione Caruso, and Santina Mangano
- Subjects
0301 basic medicine ,biology ,030106 microbiology ,Homoserine ,Biofilm ,food and beverages ,Agrobacterium tumefaciens ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Pseudomonas putida ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Quorum sensing ,Sponge ,030104 developmental biology ,chemistry ,General Agricultural and Biological Sciences ,Chromobacterium violaceum ,Bacteria - Abstract
Porifera dominate vast areas of the Antarctic shelves and are successfully colonized by bacteria. Quorum sensing (QS) is a cell-to-cell communication system based on bacterial population density that, enabling the coordination of group-based behaviour, plays a critical role in the successful colonization of higher organisms, also driving the formation of biofilm for adhesion to surfaces. In this study, the production of N-Acyl homoserine lactones (AHLs), signal molecules involved in the QS mechanism, was examined for 211 Antarctic sponge-associated Gram-negative bacteria. AHL production was screened by using three different AHL biodetection systems, i.e. Agrobacterium tumefaciens pZLR4, Chromobacterium violaceum CV026 and Pseudomonas putida pKR-C12 with optimal sensitivity to moderate-chain (C8–C12), short-chain (C4–C8) and long-chain (≥ C14) AHLs, respectively. 57.8% of tested isolates activated at least one of the monitor systems used and belonged mainly to bacterial genera that are known to be involved in surface colonization by biofilm production. A thin-layer chromatographic assay based on the A. tumefaciens reporter system was utilized to determine the AHL profiles of five selected positive isolates. Visible spots on thin-layer chromatography (TLC) plates were produced by Roseobacter sp. TB60 and Psychrobacter sp. TB67 (both from the sponge, Anoxycalyx joubini). The former probably produced N-(3-oxohexanoyl)-L-homoserine lactone (similar to the standard 3-oxo-C6-HSL), whereas the isolate TB67 produced molecules that were similar to the standard N-butanoyl-homoserine lactone (C4-HSL). The obtained results demonstrated that AHL-based signalling may play a key role in sponge–bacteria interactions also in the Antarctic environment.
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- 2018
48. Extracellular polymeric substances with metal adsorption capacity produced by Pseudoalteromonas sp. MER144 from Antarctic seawater
- Author
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Paola Di Donato, Luigi Michaud, Santina Mangano, Consolazione Caruso, Barbara Nicolaus, Angelina Lo Giudice, Carmen Rizzo, Annarita Poli, and Gaetano Di Marco
- Subjects
0301 basic medicine ,Sucrose ,Health, Toxicology and Mutagenesis ,030106 microbiology ,Antarctic Regions ,chemistry.chemical_element ,Exopolymers ,Agar plate ,Metal ,03 medical and health sciences ,chemistry.chemical_compound ,Biopolymers ,Extracellular polymeric substance ,Metals, Heavy ,Environmental Chemistry ,Seawater ,Toxicology and Mutagenesis ,Food science ,Sulfate ,Antarctica ,Cryoprotection ,Heavy metals ,Pollution ,Cadmium ,Sulfates ,Monosaccharides ,Polysaccharides, Bacterial ,Temperature ,General Medicine ,Mercury (element) ,Pseudoalteromonas ,Uronic Acids ,chemistry ,Health ,visual_art ,visual_art.visual_art_medium ,Adsorption ,Water Pollutants, Chemical - Abstract
The EPS-producing Pseudoalteromonas sp. MER144 was selected among 606 isolates from Antarctic seawater due to its evident slimy appearance on agar plates. The production of EPSs was enhanced by a step-by-step approach varying the carbon source, substrate and NaCl concentrations, temperature, and pH. Optimal conditions for the EPS production resulted at temperature of 4 °C and pH 7, with addition of 2% sucrose (w/v) and 3% NaCl (w/v). EPSs produced under optimal conditions were chemically characterized, resulting in a moderate carbohydrate content (35%), uronic acids (14%), and proteins (12%). Monosaccharide composition was estimated to be Glu:Man:GluN:Ara:GluA:GalA:Gal (1:0.36:0.26:0.06:0.06:0.05:0.03), while the estimated molecular weight was about 250 kDa. The addition of sucrose in the culture medium, by stimulating the EPS production, allowed MER144 to tolerate higher concentrations of mercury and cadmium. This finding was probably dependent on the presence of uronic acids and sulfate groups, which can bind cations, in the extracted EPSs. Monitoring EPS production under optimal conditions at different concentrations of mercury and cadmium revealed that EPS amounts increased at increasing heavy metal concentrations, indicating an adaptation to the stress conditions tested.
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- 2017
49. Bacterial isolates from the Arctic region (Pasvik River, Norway): assessment of biofilm production and antibiotic susceptibility profiles
- Author
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Maurizio Azzaro, Pasqualina Laganà, Santi Delia, Gabriella Caruso, Angelina Lo Giudice, and Ludovica Votano
- Subjects
0301 basic medicine ,Arctic region ,medicine.drug_class ,Health, Toxicology and Mutagenesis ,030106 microbiology ,Antibiotics ,bacteria, biofilm, antibiotic susceptibility, Pasvik river, Arctic region ,Environment ,010501 environmental sciences ,Bacterial Physiological Phenomena ,01 natural sciences ,biofilm ,Microbiology ,03 medical and health sciences ,Antibiotic resistance ,Rivers ,Ampicillin ,Drug Resistance, Bacterial ,medicine ,Environmental Chemistry ,Cefoxitin ,bacteria ,0105 earth and related environmental sciences ,Mezlocillin ,biology ,Arctic Regions ,Norway ,Biofilm ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Pollution ,Anti-Bacterial Agents ,Biofilms ,Sisomicin ,antibiotic susceptibility ,Pasvik river ,Bacteria ,medicine.drug - Abstract
Bacterial biofilm production is recognized as a strategy that helps aquatic bacteria in resisting to the presence of several kinds of pollutants, including antibiotics, in the bulk environment. The Pasvik River, located between Norway, Russia and Finland, is a sub-Arctic site polluted by wastes from metallurgic and mining activities. In order to study whether and to what extent bacteria are able to produce biofilms, and to assess whether this physiological characteristic influences their resistance to antibiotics, an investigation was performed on bacteria isolated from water and sediment collected along the Pasvik River course during two surveys (May and July). Bacterial strains were screened for their biofilm production and profiles of susceptibility to antibiotics. Results showed that biofilm formation was a widespread characteristic of the isolates. Most of them were also resistant to several antibiotics, such as ampicillin (100% of the isolates) as well as cefazolin, cefoxitin, ceftriaxone, mezlocillin, nitrofurantoin and sisomicin (90% of the total strains). This study shows a significant association between biofilm formation and antibiotic resistance at inner stations both in water and in sediments in May only. This suggests that in Pasvik River colder temperature may stimulate bacterial aggregation into biofilm and simultaneously decrease bacterial susceptibility to antibiotics; since the occurrence of antibiotic resistance has frequently been linked to the presence of pollutants, this result could represent a strategy of bacterial survival under altered environmental conditions.
- Published
- 2017
50. Evaluation of the changes of orbital cavity volume and shape after tooth-borne and bone-borne rapid maxillary expansion (RME)
- Author
-
Lo Giudice, Antonino, primary, Rustico, Lorenzo, additional, Ronsivalle, Vincenzo, additional, Nicotra, Carmelo, additional, Lagravère, Manuel, additional, and Grippaudo, Cristina, additional
- Published
- 2020
- Full Text
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