1. Analysis of the androgen receptor–regulated lncRNA landscape identifies a role for ARLNC1 in prostate cancer progression
- Author
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Susan M. Freier, Mats Ljungman, Lanbo Xiao, Sahr Yazdani, Kristin M. Juckette, Andrew T. Watt, Mona Batish, Shuling Guo, Alexander R. Gawronski, Saravana M. Dhanasekaran, Sudhanshu Shukla, John T. Wei, Michael Uhl, Rohit Malik, Hui Jiang, Yasuyuki Hosono, Yashar S. Niknafs, Yuanyuan Qiao, Utsav Patel, Sethuramasundaram Pitchiaya, Rohit Mehra, Lakshmi P. Kunju, Michelle T. Paulsen, Felix Y. Feng, Rolf Backofen, Xia Jiang, Xuhong Cao, Shruthi Subramaniam, Cenk Sahinalp, Tzu-Ying Liu, Jean C.-Y. Tien, Matthew K. Iyer, Girish C. Shukla, Arul M. Chinnaiyan, Yajia Zhang, Marcin Cieślik, and Lisha Wang
- Subjects
Male ,0301 basic medicine ,Biology ,Medical and Health Sciences ,Article ,Cell Line ,Androgen ,Transcriptome ,03 medical and health sciences ,Prostate cancer ,Prostate ,androgen receptor ,Cell Line, Tumor ,Receptors ,Genetics ,medicine ,Humans ,Regulation of gene expression ,Neoplastic ,Gene knockdown ,Tumor ,long non-coding RNA ,ARLNC1 ,Prostatic Neoplasms ,Biological Sciences ,prostate cancer ,medicine.disease ,Long non-coding RNA ,3. Good health ,Gene Expression Regulation, Neoplastic ,Androgen receptor ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Receptors, Androgen ,Disease Progression ,Androgens ,Cancer research ,RNA ,RNA, Long Noncoding ,Long Noncoding ,Signal transduction ,Signal Transduction ,Developmental Biology - Abstract
The androgen receptor (AR) plays a critical role in the development of the normal prostate as well as prostate cancer. Using an integrative transcriptomic analysis of prostate cancer cell lines and tissues, we identified ARLNC1 (AR-regulated long noncoding RNA 1) as an important long noncoding RNA that is strongly associated with AR signaling in prostate cancer progression. Not only was ARLNC1 induced by the AR protein, but ARLNC1 stabilized the AR transcript via RNA-RNA interaction. ARLNC1 knockdown suppressed AR expression, global AR signaling and prostate cancer growth in vitro and in vivo. Taken together, these data support a role for ARLNC1 in maintaining a positive feedback loop that potentiates AR signaling during prostate cancer progression and identify ARLNC1 as a novel therapeutic target.
- Published
- 2018
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