1. [Untitled]
- Author
-
Karen M. Walters-Thompson and William D. Mason
- Subjects
Pharmacology ,Chromatography ,medicine.diagnostic_test ,Chemistry ,Elution ,Organic Chemistry ,Extraction (chemistry) ,Diphenhydramine ,Pharmaceutical Science ,High-performance liquid chromatography ,Spectrophotometry ,Orphenadrine ,medicine ,Molecular Medicine ,Pharmacology (medical) ,Gas chromatography ,Biotechnology ,medicine.drug ,Whole blood - Abstract
An HPLC/GC-MSD method for the determination of diphenhydramine in rabbit whole blood has been developed and validated. This method is based on a liquid–liquid extraction and reversed-phase Chromatography with ultraviolet absorbance detection monitored at 258 nm. HPLC eluant fractions containing diphenhydramine and the internal standard, orphenadrine, were collected, reex-tracted, then subjected to GC-MSD analysis. Whole blood was utilized, thereby decreasing the required sample volume and increasing the sensitivity of the assay. Diphenhydramine concentrations can be quantitated over a range of 1 to 1000 ng/ml whole blood.
- Published
- 1992