1. A direct sandwich enzyme-linked limmunosorbent assay for human serum apolipoprotein A-I
- Author
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Byeong-Cheol Jeon, Young-Do Kim, Ju Won Kwak, Myung-Hoon Lee, Soo-Kyung Kim, Jin Q Kim, and Nam-Wook Jeong
- Subjects
chemistry.chemical_classification ,Apolipoprotein B ,biology ,Chemistry ,Clinical Biochemistry ,Serum albumin ,Biochemistry ,Molecular biology ,Standard curve ,Enzyme ,Polyclonal antibodies ,biology.protein ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,Apolipoprotein A1 ,Antibody ,Molecular Biology ,Peroxidase - Abstract
Apolipoprotein (apo) A-I, an abundant protein in high-density lipoproteins (HDL), has received considerable clinical attention as a negative predictor of coronary artery disease, because the plasma concentration of apo A-I has been shown to be inversely correlated with the incidence of coronary artery disease. A direct sandwich enzyme-linked immunosorbent assay (ELISA) for determination of the level of human serum apo A-I was developed using highly purified anti-apo A-I polyclonal antibodies raised in rabbits as the capturing antibody, and as the detecting antibody as well. Microtiter plates were coated with purified anti-apo A-I polyclonal antibodies. The concentration of apo A-I in samples was determined from the peroxidase color reaction. The sensitivity of the assay was determined to be 10 ng of apo A-I per ml of serum. The working range, where the standard curve showed a linearity, was between 10 and 100 ng/ml. The mean intra-assay and inter-assay coefficients of variation (CV) were
- Published
- 1996