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A rapid analytical method for sequential separation and determination of the most significant α- and β-emitting radionuclidic impurities in 99Tc radiopharmaceutical raw material is presented. Successive HTiO co-precipitation steps were first conducted for effective removal of 99Tc to avoid its spectral interference on the determination of β-emitting nuclides by liquid scintillation counting. A sequential chromatographic method was applied for simultaneous separation and purification of actinide and lanthanide isotopes. A series of K99TcO4 solution spiked with known quantities of 238Pu, 239Pu, 241Pu, 237Np, 241Am, 244Cm and 147Pm were analyzed for method evaluation, demonstrating that the method would meet the analytical requirements for radionuclidic impurity evaluation of K99TcO4 raw material.

Spermatogonial stem cells (SSCs) are crucial for maintaining spermatogenesis, studying germ line stem cell biology, and producing transgenic animals. Growth factors, including leukemia inhibitory factor (LIF), epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), and glial cell line-derived neurotrophic factor (GDNF), are essential for in vitro culture of SSCs as well as their self-renewal and maintenance. In this study, we investigated the effects of these growth factors on porcine spermatogonial germ cell (pSGC) colony formation. We determined round alkaline phosphatase (AP)-positive pSGC colonies in the presence and absence of growth factors after 7 days of pSGC culture. EGF was found to be essential to support the formation of AP-positive pSGC colonies. The expression of epidermal growth factor receptor (EGFR) and Erb-B2 receptor tyrosine kinase 2 (ERBB2) was also altered in cultured pSGCs compared to that in feeder cells. We verified the effect of EGF signaling on pSGC colony formation using AG1478 as an EGFR inhibitor and AG879 as an ERBB2 inhibitor. pSGC colonies were observed in low dose AG1478-treated groups with EGF, whereas a high dose of AG1478 suppressed pSGC colony formation. AP-positive colonies were also observed in all AG879-treated groups. Taken together, EGFR and EGF signaling play a critical role in the initiation of colony formation of pSGCs. Our study provides insights into the mechanisms of EGF-mediated colony formation by SGCs derived from porcine testes, and will aid the development of transplantation techniques for the production of transgenic offspring.

Immunotherapies have been explored in treating solid tumors, albeit with disparate clinical effects in distinct cancer types. Systematic interrogation of immune cells in the tumor microenvironment (TME) is vital to the prediction of immunotherapy response and the development of innovative immunotherapeutics. To comprehensively characterize the immune microenvironment in advanced biliary tract cancer (BTC), we utilized single-cell RNA sequencing in unselected viable cells from 16 matched samples, and identified nineteen cell subsets from a total of 45,851 cells, in which exhausted CD8+ T cells, macrophages, and dendritic cells (DCs) in BTC were shown to augment and communicate within the TME. Transcriptional profiles coupled with T cell receptor (TCR) sequences revealed that exhausted CD8+ T cells retained clonal expansion and high proliferation in the TME, and some of them highly expressed the endoplasmic reticulum stress (ER) response gene, XBP1, indicating the role of ER stress in remodeling TME. Functional assays demonstrated that XBP1 and common immune checkpoints (PD1, TIGIT) were significantly upregulated in CD8+ T cells cocultured within the TME of BTC cells (GBC-SD, HCCC-9810). When treating the coculture groups with the specific inhibitor of IRE1α-XBP1 (4μ8C), the downregulation of TIGIT was observed in the treatment group. Collectively, comprehensive transcriptome profiling provides deep insights into the immune atlas in advanced BTC, which might be instrumental in exploring innovative immunotherapy strategies.

Background Anti-IgLON5 disease is a rare neurological disorder associated with autoantibodies against the neuronal cell adhesion protein, IgLON5. Cellular investigations with human IgLON5 antibodies have suggested an antibody-mediated pathogenesis, but whether human IgLON5 autoantibodies can induce disease symptoms in mice is yet to be shown. Moreover, the effects of anti-IgLON5 autoantibodies on neurons and the precise molecular mechanisms in vivo remain controversial. Methods We investigated the effects of anti-IgLON5 antibodies in vivo and evaluated their long-term effects. We used two independent passive-transfer animal models and evaluated the effects of the antibodies on mouse behaviors at different time points from day 1 until day 30 after IgG infusion. A wide range of behaviors, including tests of locomotion, coordination, memory, anxiety, depression and social interactions were established. At termination, brain tissue was analyzed for human IgG, neuronal markers, glial markers, synaptic markers and RNA sequencing. Results These experiments showed that patient’s anti-IgLON5 antibodies induced progressive and irreversible behavioral deficits in vivo. Notably, cognitive abnormality was supported by impaired average gamma power in the CA1 during novel object recognition testing. Accompanying brain tissue studies showed progressive increase of brain-bound human antibodies in the hippocampus of anti-IgLON5 IgG-injected mice, which persisted 30 days after the injection of patient’s antibodies was stopped. Microglial and astrocyte density was increased in the hippocampus of anti-IgLON5 IgG-injected mice at Day 30. Whole-cell voltage clamp recordings proved that anti-IgLON5 antibodies affected synaptic homeostasis. Further western blot investigation of synaptic proteins revealed a reduction of presynaptic (synaptophysin) and post-synaptic (PSD95 and NMDAR1) expression in anti-IgLON5 IgG-injected mice. Conclusions Overall, our findings indicated an irreversible effect of anti-IgLON5 antibodies and supported the pathogenicity of these antibodies in vivo.

This study was to investigate clinical applicability of diffusion spectrum imaging (DSI) for quantitative detection of visual pathway abnormalities to predict the degree of visual field defects (VFD) in patients with pituitary adenomas. Sixty-five patients with pituitary adenomas and 33 healthy controls underwent conventional MRI and DSI scanning that allowed high-angular-resolution fiber tracking. Optic chiasmal compression and VFD were confirmed in all patients via radiological and neuro-ophthalmological examinations. Quantitative assessments of chiasmal lift, VFD, and DSI parameters from the optic nerve, optic tract, and optic radiation were performed. Group comparisons and correlation analyses were conducted in patients and controls. Using the 5-fold cross-validation method, the support vector machine classifiers were constructed to predict the degree of visual defects. The mean values of quantitative anisotropy and generalized fractional anisotropy in optic nerve and optic tract showed significant differences between patients and controls (p < 0.05). These parameters were also significantly correlated with the chiasmal lift distance and degree of visual defects (p < 0.05). All patients were divided into mild (n = 42) and severe (n = 23) VFD groups, using the mean deviation value of −8 dB as the threshold. The classifiers achieved an accuracy of 0.83, sensitivity of 0.78, and specificity of 0.86 to discriminate patients with mild and severe visual defects. Using high-angular-resolution fiber tracking, DSI may provide quantitative information to detect visual pathway abnormalities and be a potential diagnostic tool for determining the degree of visual field defects in pituitary adenomas. • Abnormal QA and GFA values of optic nerve and optic tract in adenoma patients • Close relationship between DSI parameters and VFD degree in adenoma patients • The classifiers achieved an accuracy of 0.83, sensitivity of 0.78, and specificity of 0.86 to discriminate patients with mild and severe VFD

Chronic inflammation of autoimmune diseases, including type 1 diabetes (T1D), is mainly mediated by memory T(Tm) cells, predominantly effector memory T (Tem) cells. The roles of the programmed death-1 (PD-1) receptor on lymphocytes have been well studied in tumor and other infection models. However, little is known about the relationship between the expression of PD-1 on CD8+ Tem cells and the pathogenesis of T1D. A total of 52 patients diagnosed with T1D and 39 gender-, age-, and ethnically matched health control individuals were enrolled in this study. Peripheral blood mononuclear cells from these individuals were isolated and analyzed by flow cytometry. We evaluated the frequencies of PD-1+ CD8+ memory T cell subsets from patients' peripheral blood with T1D and the spleen cells of nonobese diabetic (NOD) mice in the present study. We also investigated the effects of blocking PD-1/PD-L1 pathway on islet’s inflammation in NOD mice. Frequencies of PD-1+ CD8+ Tem cells were decreased significantly in PBMC of patients with T1D (40.73 ± 12.72 vs 47.43 ± 15.56, *p

Angiogenesis is an integral process in many ischemic disorders, and vascular endothelial growth factor (VEGF) plays an important role in it. Protein arginine methyltransferase 4 (PRMT4), a member of the type I PRMT family, is involved in various biological activities, but its role in endothelial cell (EC) remains elusive. Here, we aimed to investigate the role of PRMT4 in ischemic angiogenesis and explore the possible underlying mechanism. We found that PRMT4 was upregulated in ischemic muscles, and VEGF treatment potentiated its expression in ECs. In vitro, adenovirus-mediated PRMT4 overexpression promoted, while its gene disruption inhibited, EC proliferation, migration, and tube formation. In an in vivo hindlimb ischemia model, forced expression of PRMT4 in ECs showed accelerated blood flow recovery and increased capillary density, whereas its knockdown exhibited the opposite effect. Mechanistically, PRMT4 activated the transcription of VEGF via the interaction with Y-box binding protein-1 (YB1), leading to accelerated angiogenesis. Interestingly, the loss of YB1 partially abolished PRMT4-mediated angiogenesis in vitro. Collectively, our data revealed that PRMT4 promoted angiogenesis through interacting with YB1 and the consequential VEGF upregulation, suggesting that PRMT4 may present as a potential therapeutic target in ischemic angiogenesis. KEY MESSAGES: •PRMT4 is induced by VEGF and upregulated in a hindlimb ischemia model. •PRMT4 promotes angiogenesis both in vitro and in vivo. •PRMT4 regulates VEGF expression through interacting with YB1. •YB1 knockdown retards PRMT4-mediated angiogenic effects in vitro.

It has been speculated that brain activities might directly control adaptive immune responses in lymphoid organs, although there is little evidence for this. Here we show that splenic denervation in mice specifically compromises the formation of plasma cells during a T cell-dependent but not T cell-independent immune response. Splenic nerve activity enhances plasma cell production in a manner that requires B-cell responsiveness to acetylcholine mediated by the α9 nicotinic receptor, and T cells that express choline acetyl transferase1,2 probably act as a relay between the noradrenergic nerve and acetylcholine-responding B cells. We show that neurons in the central nucleus of the amygdala (CeA) and the paraventricular nucleus (PVN) that express corticotropin-releasing hormone (CRH) are connected to the splenic nerve; ablation or pharmacogenetic inhibition of these neurons reduces plasma cell formation, whereas pharmacogenetic activation of these neurons increases plasma cell abundance after immunization. In a newly developed behaviour regimen, mice are made to stand on an elevated platform, leading to activation of CeA and PVN CRH neurons and increased plasma cell formation. In immunized mice, the elevated platform regimen induces an increase in antigen-specific IgG antibodies in a manner that depends on CRH neurons in the CeA and PVN, an intact splenic nerve, and B cell expression of the α9 acetylcholine receptor. By identifying a specific brain–spleen neural connection that autonomically enhances humoral responses and demonstrating immune stimulation by a bodily behaviour, our study reveals brain control of adaptive immunity and suggests the possibility to enhance immunocompetency by behavioural intervention. Neuronal activities in the central amygdala and paraventricular nucleus are transmitted via the splenic nerve to increase plasma cell formation after immunization, and this process can be behaviourally enhanced in mice.

The TIM family comprises of eight genes in the mouse, three of which are conserved in humans (TIM-1, TIM-3, and TIM-4). Previous studies have revealed the relationships between Tim3+ Tregs and autoimmune disease. There was little study about the expression of Tim1 and Tim4 surface molecules on Tregs. We evaluated the frequency of the Tim1+Tregs and Tim4+Tregs in type 1 diabetes (T1D) in the present study. A total of 28 patients with T1D and 14 gender-, age-, and ethnically matched healthy volunteers were recruited. PBMCs from these individuals were isolated and analyzed by flow cytometry. Splenocytes from mice were also analyzed by flow cytometry. There is no difference in the frequency of Treg cells in peripheral blood isolated from T1D patients. Tim1 on CD4+CD25+ T cells decreased significantly in PBMC of patients with T1D(1.19 ± 0.17% vs 2.78 ± 0.38%, 95% CI:0.87–2.31, P

Knowledge about organic carbon loadings (ratio of sedimentary organic carbon (SOC) content to specific surface area (SSA)) and the fate of organic carbon (OC) is critical to understand the marine carbon cycle. We investigated the variations in the patterns of OC loadings and the preservation capacities of sedimentary OC in the Yap Trench and other marine environments. The average OC loading in sediment cores from various marine environments decreases with increasing water depth at a rate of ~0.06 mg OC/(m2·km) (R2 =0.23, P

The neural substrates for predatory hunting, an evolutionarily conserved appetitive behavior, remain largely undefined. Photoactivation of zona incerta (ZI) GABAergic neurons strongly promotes hunting of both live and artificial prey. Conversely, photoinhibition of these neurons or deletion of their GABA function severely impairs hunting. Here electrophysiological recordings reveal that ZI neurons integrate prey-related multisensory signals and discriminate prey from non-prey targets. Visual or whisker sensory deprivation reduces calcium responses induced by prey introduction and attack and impair hunting. ZI photoactivation largely corrects the hunting impairment caused by sensory deprivations. Motivational and reinforcing assays reveal that ZI photoactivation is associated with a strong appetitive drive, causing repetitive self-stimulatory behaviors. These ZI neurons project to the periaqueductal gray matter to induce hunting and motivation. Thus, we have delineated the function of ZI GABAergic neurons in hunting, which integrates prey-related sensory signals into prey detection and attack and induces a strong appetitive motivational drive.

Purpose This essay aims to propose suggestions on what we can learn from previous investigations to conduct further studies on the potential mechanisms underlying the effect of diabetes mellitus on COVID-19. Methods We reviewed some literature on diabetes and other types of coronavirus infection such as Middle East respiratory syndrome (MERS) and severe acute respiratory syndrome (SARS) and made some summaries and comparisons. Results Diabetes affect the occurrence and progression of COVID-19. Conclusions In-depth and comprehensive exploration of the mechanism of diabetes affecting COVID-19 should be carried out.

The development of multicore hardware has provided many new development opportunities for many application software algorithms. Especially, the algorithm with large calculation volume has gained a lot of room for improvement. Through the research and analysis, this paper has presented a parallel PO-Dijkstra algorithm for multicore platform which has split and parallelized the classical Dijkstra algorithm by the multi-threaded programming tool OpenMP. Experiments have shown that the speed of PO-Dijkstra algorithm has been significantly improved. According to the number of nodes, the completion time can be increased by 20–40%. Based on the improved heterogeneous dual-core simulator, the Dijkstra algorithm in Mi Bench is divided into tasks. For the G.72 encoding process, the number of running cycles using “by function” is 34% less than using “divided by data,” while the power consumption is only 83% of the latter in the same situation. Using “divide by data” will reduce the cost and management difficulty of real-time temperature. Using “divide by function” is a good choice for streaming media data. For the Dijkstra algorithm, the data is data without correlation, so using a simpler partitioning method according to the data partitioning can achieve good results. Through the simulation results and the analysis of the results of real-time power consumption, we conclude that for data such as strong data correlation of streaming media types, using “divide by function” will have better performance results; for data types where data correlation is not very strong, the effect of using “divide by data” is even better.

Screening high-risk individuals for gestational diabetes mellitus (GDM) in early pregnancy conventionally relies on established maternal risk factors; however, the sensitivity and specificity of these factors are not satisfactory. The present study aimed to determine whether the concentration of angiopoietin-like protein 8 (ANGPTL8), either alone or combined with other risk factors in early pregnancy, could be used to predict subsequent GDM. From August 2015 to January 2016, 474 women receiving prenatal care at around 12–16 weeks of gestation were recruited into the study. ANGPTL8 levels were measured at the first prenatal visit. All the participants received a 75 g OGTT during weeks 24–28 of gestation. ANGPTL8 levels in early pregnancy were considerably higher in women who developed GDM than those who maintained normal glucose tolerance (2822 ± 938 vs 2120 ± 1118 pg/ml, respectively; p

Magnetic resonance spectroscopy of single biomolecules under near-physiological conditions may substantially advance understanding of biological function, yet remains very challenging. Here we use nitrogen-vacancy centers in diamonds to detect electron spin resonance spectra of individual, tethered DNA duplexes labeled with a nitroxide spin label in aqueous buffer solutions at ambient temperatures. This paves the way for magnetic resonance studies on single biomolecules and their inter-molecular interactions in a native-like environment., 22 pages, 14 figures

SHP099, a selective inhibitor of signalling meditator SHP2 with drug-like properties, has an allosteric mechanism of action whereby it stabilizes SHP2 in an auto-inhibited conformation, and suppresses RAS–ERK signalling and proliferation in receptor-tyrosine-kinase-driven cancer cell lines and mouse tumour xenograft models. The tyrosine phosphatase SHP2 is a key mediator of receptor tyrosine kinase (RTK) signalling, as well as being important in immune checkpoint pathways. Reduction of SHP2 activity suppresses tumour cell growth, and SHP2 is a potential, but so far elusive, therapeutic target in cancer. Pascal Fortin and colleagues report the development of a selective SHP2 inhibitor with drug-like properties. The inhibitor, SHP099, has an allosteric mechanism of action whereby it stabilizes SHP2 in an auto-inhibited conformation. It also suppresses RAS–ERK signalling to inhibit RTK-driven proliferation in human cancer cell lines and mouse tumour xenograft models. The non-receptor protein tyrosine phosphatase SHP2, encoded by PTPN11, has an important role in signal transduction downstream of growth factor receptor signalling and was the first reported oncogenic tyrosine phosphatase1. Activating mutations of SHP2 have been associated with developmental pathologies such as Noonan syndrome and are found in multiple cancer types, including leukaemia, lung and breast cancer and neuroblastoma1,2,3,4,5. SHP2 is ubiquitously expressed and regulates cell survival and proliferation primarily through activation of the RAS–ERK signalling pathway2,3. It is also a key mediator of the programmed cell death 1 (PD-1) and B- and T-lymphocyte attenuator (BTLA) immune checkpoint pathways6,7. Reduction of SHP2 activity suppresses tumour cell growth and is a potential target of cancer therapy8,9. Here we report the discovery of a highly potent (IC50 = 0.071 μM), selective and orally bioavailable small-molecule SHP2 inhibitor, SHP099, that stabilizes SHP2 in an auto-inhibited conformation. SHP099 concurrently binds to the interface of the N-terminal SH2, C-terminal SH2, and protein tyrosine phosphatase domains, thus inhibiting SHP2 activity through an allosteric mechanism. SHP099 suppresses RAS–ERK signalling to inhibit the proliferation of receptor-tyrosine-kinase-driven human cancer cells in vitro and is efficacious in mouse tumour xenograft models. Together, these data demonstrate that pharmacological inhibition of SHP2 is a valid therapeutic approach for the treatment of cancers.

The synthesis and characterization of SnO2 nanomaterials have been extensively studied as photoelectrodes for the potential applications in optoelectronic devices. In this work, SnO2 nanorods arrays have been synthesized by hydrothermal method on the nanoporous surface of the anodized stainless steel. The prepared SnO2 nanorods of 1.3–1.4 µm in length and 250–350 nm in width, were uniformly distributed on the anodized stainless steel. This one-dimensional SnO2 nanostructure directly fabricated on the substrate provides an electron transfer pathway and a Schottky-type contact, resulting in improved photocatalytic and photoelectrochemical performance. The SnO2 nanorods arrays exhibit fast response towards H2O2 determination, producing a linear range from 100 to 3000 μM with a correlation coefficient of 0.984 and a sensitivity of 0.66 μA cm−2 mM−1. The results indicate the potential applications of SnO2 nanorods arrays as the non-enzymatic H2O2 sensor.

Purpose Knowledge of archaeal communities is essential for understanding of the mechanism of carbon and nitrogen cycle in the mangrove sediment ecosystem. Presently, little is known about archaeal communities in the Dongzhaigang mangrove sediments. This study aimed to characterize the archaeal communities in sediments of different mangrove stands and to find out the correlations between archaeal communities and the environmental factors of sediments.

Cobalt-modified manganese–zinc (MnZn) power ferrites with good thermal stability were prepared by conventional ceramic technique. The influences of TiO2 and Nb2O5 additives on the properties of cobalt-modified MnZn power ferrite were investigated. It was shown that moderate amount of TiO2 and Nb2O5 additives could increase the permeability and reduce the power loss effectively while keeping the better thermal stability of cobalt-modified MnZn ferrite. The best magnetic properties were obtained at addition amount of 0.03 wt% TiO2 and 0.02 wt% Nb2O5 with an initial permeability (µ i ) of 3200 at room temperature and power loss keeping below 400 kW/m3 from 20 to 100 °C. The related mechanisms were briefly discussed.

Despite the great number of studies about methane uptake and its response to grazing in the Inner Mongolia grasslands, only a few focused on the methanotrophic composition. This study aimed to investigate the methanotrophic community structure and abundance, then to analyze the abiotic driving factors of methanotrophic community structure in different enclosed times in this area. In this study we chose typical grasslands in the Xilin River Basin of Inner Mongolia, China to investigate methanotrophic community structure and abundance under different enclosure treatments as follows: 79E (grassland enclosed since 1979), 99E (grassland enclosed since 1999), and G (freely grazed grassland). A clone library was used to reveal the methanotroph community structure, and their relationships with abiotic factors were analyzed by redundancy analysis. Methanotroph abundance was determined by real-time PCR. The OTUs of the three treatments mainly belonged to Type I methanotrophs, probably caused by the high pH value. Among all OTUs, only OTU1 belonged to upland soil cluster γ (USC-γ), whose abundance was the largest in all OTUs, indicating the USC-γ cluster was the main one to oxidize CH4 in the Inner Mongolia grasslands. Methanotrophic abundance (represented by the pmoA gene copies per gram of dry weight soil) decreased with the enclosure time as G (4.5 × 107) > 99E (2.8 × 107) > 79E (2.0 × 107), mainly caused by the lower soil moisture content in G. Lower soil moisture content facilitates more CH4 and O2 diffusive into soil thus leading to the proliferation of methanotrophs. This study found a high abundance of methanotrophs in the soils of the Inner Mongolia grasslands, with the USC-γ cluster having the largest abundance, which may play a key role in oxidizing CH4 in the Inner Mongolia grasslands. Combined with those of previous studies, the results showed an obvious change of methanotrophic community composition with the increase of enclosure time.

The governing equations of flexible hoisting rope are developed employing Hamilton’s principle. Experiments are performed. It is found that the experimental data agree with the theoretical prediction very well. The results of simulation and experiment show that the flexible hoisting system dissipates energy during downward movement but gains energy during upward movement. Further, a passage through resonance in the hoisting system with periodic external excitation is analyzed. Due to the time-varying length of the hoisting rope the natural frequencies of the system vary slowly, and transient resonance may occur when one of frequencies coincides with the frequency of external excitation. Основні рівняння гнучкого підіймального тросу отримано застосуванням принципу Гамільтона. Проведено експерименти, результати яких добре узгоджуються з теоретичним передбаченням. Результати моделювання і експеримент показують, що гнучка підіймальна система розсіює енергію при спуску і накопичує енергію при підйомі. Далі досліджувався перехід гнучкої підіймальної системи через резонанс за умови періодичного зовнішнього збудження. Якщо довжина гнучкої підіймальної системи змінюється з часом, то власні частоти системи слабо змінюються і можуть спостерігатися перехідні резонанси, коли одна з частот співпадає з частотою зовнішнього збудження.

Two records of the crust laminae from the Marcus-Wake Seamounts and the Magellan Seamount were biostratigraphically studied. Based on biological imprints of the calcareous nannofossils, the geological ages of the two records were determined, with CM1D03 from the Marcus-Wake Seamounts being of late Paleocene to Pleistocene and CM3D06 from the Magellan Seamount of Late Cretaceous (more than 70.0 Ma). There are the obvious temporal-spatial differences in the initial formation period and enrichment characteristics of the cobalt-rich crusts of the two seamount chains and differences in the combination and distribution of microfossils in the inner crust layers between the seamounts. These differences are due to the adaptabilities of oceanic species in different environments. Ecological research was carried out in terms of population size of the calcareous nannofossils preserved in the crustal layers to discern the relation of the geological events at the Eocene-Oligocene (E/O) transition. The results show the transitions and recombination of species in the biotic community during the E/O transition obviously corresponded to 25 mm depth in the CM1D03 crust and 58 mm depth in the CM3D06 crust. The changes in biological species and the formation of particular ecological structures indicate the adaptive response of the paleo-biological community in the western Pacific Ocean to the global cold-climate events and the close correlation between the formation of the crust and the global climate change.

Annual thawing/freezing indices (TIs and FIs) for ten meteorological stations in the China Tianshan Mountains (CTM) are analyzed based on the daily maximum and minimum temperature records during 1961–2010. These indices are submitted for trend analysis and change-point analysis. The correlations between the TIs and FIs and the annual mass balance (AMB) for the No. 1 glacier, a rapid regressing small-scale glacier with the solely longest observed time series of the AMB at the headwater of the Urumqi River in the CTM, were analyzed in order to demonstrate the implication behind variations in the TIs and FIs. Furthermore, to explain atmospheric controls of the TI and FI variations, correlations between large-scale climatic forcing, e.g., the mean NAO indices and the mean Arctic Oscillation (AO) and the TIs and the FIs were analyzed. The results show that there were obvious upward trends in the TIs and downward trends in the FIs occurred, which demonstrates an increasing thawing and decreasing freezing potential in the CTM during the period of 1961–2010. Change-point analysis revealed evident change point around the year 1997 for the TIs at most of the stations, while various change points for the FIs. Obvious negative relationships between the TIs and the AMB for the No. 1 glacier have been found. Also, significant change point around the year 1996/1997 has been detected for the AMB time series. The revealed tight links between the TIs and the AMB, and also temporal consistency of change points indicates that changes in the TIs could be one of the main factors resulted in changes in the AMB of No. 1 glacier. Furthermore, significant negative correlation between the TIs and FIs and the mean summer NAO index for most stations are also found, demonstrating that the variations in TIs and FIs in the CTM were possibly related with changes in NAO.

The nonlinear vibration of a flexible hoisting rope with time-varying length is investigated. The governing equations of the flexible hoisting rope are developed based on Hamilton’s principle. Experiments performed evaluated the theoretical model and found that the experimental data agree well with the theoretical prediction, which validates the mathematical model of the flexible hoisting system. The results of the simulations and experiments show that the flexible hoisting system dissipates energy during downward movement (thus is stabilized) and gains energy during upward movement (thus is unstabilized). In addition, a passage through resonance in the hoisting system with periodic external excitation is analyzed. Due to the time-varying length of the hoisting rope the natural frequencies of the system vary slowly, and transient resonance may occur when one of the frequencies coincides with the frequency of an external excitation.

Tropical forests play an important role in carbon cycle. However, the temporal and spatial variation in soil carbon dioxide (CO2) emission of tropical forest remains uncertain, especially near the Tropic of Cancer. In this research, we studied the annual soil CO2 fluxes from three tropical montane rainforests on the Hainan Island of China (pristine montane rainforest, PF; secondary montane rainforest, SF; and Podocarpus imbricatus plantation, PP). The results showed a lower annual average soil CO2 flux as 6.85 ± 0.52 Mg C-CO2 ha−1 (9.17 Mg C-CO2 ha−1 in the wet season and 4.50 Mg C-CO2 ha−1 in the dry season). The CO2 fluxes exhibited obviously seasonal variation during the study period. Among the three forest types, PF had the highest average CO2 flux rate of 317.77 ± 147.71 mg CO2 m−2 h−1 (433.08 mg CO2 m−2 h−1 in the wet season and 202.47 mg CO2 m−2 h−1 in the dry season), followed by PP of 286.84 ± 137.48 mg CO2 m−2 h−1 (367.12 mg CO2 m−2 h−1 in the wet season and 206.56 mg CO2 m−2 h−1 in the dry season) and SF of 255.09 ± 155.26 mg CO2 m−2 h−1 (351.48 mg CO2 m−2 h−1 in the wet season and 155.71 mg CO2 m−2 h−1 in the dry season). We found between CO2 fluxes and soil temperature a highly significant linear relation (P 0.05). The CO2 flux was significantly correlated (P

The spatial–temporal variability of the precipitation extremes defined by eight precipitation indices based on daily precipitation dataset was analyzed using the linear regression method and the Mann–Kendall test. The results indicate that increasing trends in the precipitation amount, rainy days, and the intensity of the extreme precipitation were identified at above 70 % of the total rain stations considered in this study, with more than 30 % of them were significant, while most stations show notable decreasing trend in the annual maximum consecutive no-rain days. Significantly increasing trends of the precipitation extremes are observed mainly in the northern Xinjiang and the north of the southern Xinjiang. Most extreme precipitation indices show a potential regime shift starting from the middle of 1980s. The magnitude of the trends is compatible with their pattern of spatial stability. The generally increasing trends in precipitation extremes are found in this study.

Paramagnetic centers of the solid superacid catalyst in the sulfated TiO2 are prone to the electron paramagnetic resonance (EPR) spectroscopy. The induction of the catalytic-active sites in TiO2 powder presubmerged in H2SO4 solution as a function of the calcinated temperature of 293–873 K is investigated by X-band in situ continuous-wave EPR measurements. Sulfated-acid sites composed of the Ti3+ ion are formed upon calcination. The overall experimental results show that the population of these sites goes uphill with the elevating temperature, reaches a maximum at ~623 K and decreases afterward to close zero. During the process, the decomposition of the TiO2/SO4 2− leads to the formation of Ti3+ species and then to the increasing EPR signal amplitude, and the consecutive decomposition of the sulfur at higher temperature (>650 K) to the diminishing signal. The X-ray diffraction indicates that the introduction of SO4 2− stabilizes the geometric structure in the anatase phase.

In this study, we assess spatial patterns of variations and trends of onset, cessation, and length of growing season using mean air temperature data recorded at 51 stations in Xinjiang province, NW China over the period 1959–2008. Rank-based Mann–Kendall trend test and linear regression method are used to detect the significance and the magnitude of growing season change, respectively. Regionally, the average onset of the growing season has shifts 5.3 days earlier while the average ending date has moved 7.1 days later, increasing the length of the growing season by an average of 12.6 days. This study reveals a quite different result from previous studies. While the lengthening of the growing season in Xinjiang in the past 50 years is similar to that of previous studies, we find that the lengthening can be mainly attributed to delay of cessation in autumn rather than advance of onset in spring. The extended growing season will have strong implications in regional agricultural production of Xinjiang.

This paper introduces the 3D numerical simulation of unsteady turbulent flow in the entire flow passage of a Francis turbine with computational fluid dynamics (CFD) technology. The boundary conditions have been implemented based on the 3D averaged Navier-Stokes equations. The governing equations are discreted on space by the finite volume method and on time step by the finite difference method. The 3D unsteady turbulent flow in an entire Francis turbine model is calculated successfully using the CFX-TASCflow software and RNG k-ɛ turbulence model. Transient flow fields are simulated in the spiral case, the distributor, the runner, and the draft tube for the optimum operating condition. Meanwhile, the velocity and pressure at any points in the flow fields can be obtained so as to provide the great value on the performance prediction. By means of the numerical simulation in a flow field, it is verified that every component in a Francis turbine model is designed reasonably. The basis for the further researches on hydraulic turbines is also built.

Quantum coherence is an important enabling feature underpinning quantum computation. However, because of couplings with its noisy surrounding environment, qubits suffer from the decoherence effects. The dynamical decoupling (DD) technique uses pulse-induced qubit flips to effectively mitigate couplings between qubits and environment. Optimal DD eliminates dephasing up to a given order with the minimum number of pulses. In this paper, we first introduce our recent work on prolonging electron spin coherence in γ-irradiated malonic acid crystals and analyze different decoherence mechanisms in this solid system. Then we focus on electron spin relaxation properties in another system, phosphorous-doped silicon (Si:P) crystals. These properties have been investigated by pulse electron paramagnetic resonance (EPR). We also investigate the performance of the dynamical decoupling technique on this system. Using 8-pulse periodic DD, the coherence time can be extended to 296 μs compared with 112 μs with one-pulse control.

Service Oriented Architecture (SOA) is considered to be an important enabler of Internet of Services. By adopting SOA in development, business services can be offered, mediated, and traded as web services, so as to support agile and dynamic business collaborations on the Internet. Business collaboration is often implemented as cross-enterprise processes and involves more than one business entity which agrees to join the collaboration. To enable trustworthy and secure provision of services and service composition across enterprise boundaries, trust between business participants must be established, that is, user identities and access rights must be federated, to support business functions defined in the business processes. This paper proposes an approach which derives trust federation from formally described business process models, such as BPMN and WS-CDL processes, to automate security configuration of business collaborations. The result of the derivation is trust policies which identify trust relationships between business participants and can be enforced in enterprises' service runtimes with support of a policy deployment infrastructure.

The histone deacetylase inhibitor (HDACi) suberoylanilide hydroxamic acid (SAHA) enhances taxol-induced antitumor effects against some human cancer cells. The aim of this study is to investigate whether SAHA can enhance taxol-induced cell death against human breast cancer cells and to illustrate the mechanism in detail.A panel of eight human breast cancer cell lines and an immortalized human breast epithelial cell line were used to determine the inhibitory effects of SAHA, taxol, or their combination by MTT assay. The effects of SAHA with or without taxol on cell cycle distributions, apoptosis, and protein expressions were also examined. The inhibitory effects on tumor growth were characterized in vivo in BALB/c nude mice bearing a breast cancer xenograft model.Taxol-resistant and multi-resistant breast cancer cells were as sensitive to SAHA as taxol-sensitive breast cancer cells. A dose-dependent synergistic growth inhibition was found in all the tested breast cancer cell lines treated with the SAHA/taxol combinations. The synergetic effect was also observed in the in vivo xenograft tumor model. The cell cycle analysis and apoptosis assay showed that the synergistic effects resulted from enhanced G2/M arrest and apoptosis.SAHA increased the anti-tumor effects of taxol in breast cancer in vitro and in vivo. The combination of SAHA and taxol may have therapeutic potential in the treatment of breast cancer.

In the version of this paper originally published online, the ORCID ID for Peter Z. Qin was incorrectly assigned to Zhuoyang Qin. In addition, the ORCID for Fazhan Shi was omitted. These errors have been corrected in the print, PDF, and HTML versions of the paper.