1. MicroRNA-196a-5p in Extracellular Vesicles Secreted from Myoblasts Suppresses Osteoclast-like Cell Formation in Mouse Cells
- Author
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Yoshimasa Takafuji, Hiroshi Kaji, Kiyotaka Okada, Kohei Tatsumi, Naoyuki Kawao, and Masafumi Muratani
- Subjects
0301 basic medicine ,Endocrinology, Diabetes and Metabolism ,Osteoclasts ,030209 endocrinology & metabolism ,Cell Line ,Myoblasts ,Extracellular Vesicles ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Osteoclast ,microRNA ,medicine ,Animals ,Myocyte ,Orthopedics and Sports Medicine ,Receptor ,Activator (genetics) ,Chemistry ,Cell Differentiation ,Transfection ,Mitochondria ,Cell biology ,MicroRNAs ,RAW 264.7 Cells ,medicine.anatomical_structure ,030101 anatomy & morphology ,Bone marrow ,Energy Metabolism ,C2C12 - Abstract
Muscle/bone interaction has been recently noted. Extracellular vesicles (EVs) play a vital role in physiological and pathophysiological processes by transferring microRNA (miRNA) to distant tissues. We previously reported that EVs secreted from C2C12 myoblasts (Myo-EVs) suppress osteoclast differentiation. In the present study, we identified 4 miRNAs in Myo-EVs that suppressed osteoclast-like cell formation in Raw264.7 cells using small RNA sequencing analysis. Among them, miR-196a-5p expression was higher in C2C12 cells compared to mouse osteoblasts and bone marrow cells. Transfection of miR-196a-5p mimic suppressed the mRNA levels of osteoclast-related genes and mitochondrial energy metabolism induced by receptor activator of nuclear factor-κB ligand in Raw264.7 cells. In contrast, miR-196a-5p mimic enhanced osteoblastic differentiation in ST-2 cells and MC3T3-E1 cells. In conclusion, we demonstrated that miR-196-5p suppresses osteoclast-like cell formation and mitochondrial energy metabolism in mouse cells, suggesting that it might be a crucial factor for muscle/bone interaction via EVs.
- Published
- 2020