1. Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2
- Author
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James J. Moresco, Paul Schimmel, Benjamin J. Blencowe, Jolene K. Diedrich, Alan M. Lambowitz, Ryan M. Nottingham, John R. Yates, Douglas C. Wu, Haissi Cui, and Justin J. Lim
- Subjects
Serine ,Gene isoform ,Immune system ,Arginine ,Chemistry ,Transfer RNA ,RNA splicing ,medicine ,Inflammation ,Metabolism ,Cell Biology ,medicine.symptom ,Cell biology - Abstract
Cells respond to perturbations such as inflammation by sensing changes in metabolite levels. Especially prominent is arginine, which has long known connections to the inflammatory response. Here we show that depletion of arginine during inflammation decreased levels of arginyl-tRNA synthetase (ArgRS) in the nucleus. We found that nuclear ArgRS interacted with serine/arginine repetitive matrix protein 2 (SRRM2) in membrane-less, condensate-like, SRRM2-dependent nuclear speckles. This interaction impeded SRRM2 speckle trafficking and resulted in changes in alternative mRNA splicing. Splice site usage was regulated in opposite directions by ArgRS and SRRM2. These ArgRS- and SRRM2-dependent splicing changes cumulated in synthesis of different protein isoforms that altered cellular metabolism and peptide presentation to immune cells. Our findings delineate a novel mechanism whereby a tRNA synthetase responds to a metabolic change and modulates the splicing machinery via condensate trafficking for cellular responses to inflammatory injury.
- Published
- 2023
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