1. A high-concentrate diet induced colonic epithelial barrier disruption is associated with the activating of cell apoptosis in lactating goats
- Author
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Yingdong Ni, Haibo Dong, Ruqian Zhao, Shiyu Tao, Jing Tian, and Yongqian Duanmu
- Subjects
Lipopolysaccharides ,LPS ,Lipopolysaccharide ,Colon ,Apoptosis ,HC diet ,Biology ,Andrology ,chemistry.chemical_compound ,Rumen ,Intestinal mucosa ,Lactation ,medicine ,Animals ,Dry matter ,Intestinal Mucosa ,Tight junctions ,chemistry.chemical_classification ,TUNEL assay ,General Veterinary ,Goats ,Fatty acid ,Epithelial Cells ,General Medicine ,Fatty Acids, Volatile ,Animal Feed ,veterinary(all) ,Gastrointestinal Contents ,Body Fluids ,Diet ,medicine.anatomical_structure ,Gene Expression Regulation ,chemistry ,Biochemistry ,Colonic mucosa ,Goat ,Female ,Research Article - Abstract
Background In ruminants, lower ruminal pH causes massive disruption of ruminal epithelial structure during periods of feeding high-concentrate diets. However, the influence of excessive organic fatty acids in the lumen of hindgut on the epithelial structure is unclear. In this study, twelve mid-lactating goats were randomly assigned to either a HC diet group (65% concentrate of dry matter; n = 6) or a LC diet group (35% concentrate of dry matter; n = 6) for 10 weeks. The colonic epithelial structure was detected by HE staining and transmission electron microscopy (TEM), and the apoptotic status of epithelial cells was estimated by TUNEL method and caspase activities. Results HC goats showed higher level of free lipopolysaccharide (LPS) in rumen fluid (p 0.05), and higher total volatile fatty acid (VFA) concentrations in rumen fluid (p 0.05). Conclusions Our results reveal that long-term feeding HC diet to lactating goats causes severe damages to the colonic mucosa barrier associated with activating cells apoptosis. Electronic supplementary material The online version of this article (doi:10.1186/s12917-014-0235-2) contains supplementary material, which is available to authorized users.
- Published
- 2014