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10 results on '"Greb, Thomas"'

1. OBERON3 and SUPPRESSOR OF MAX2 1-LIKE proteins form a regulatory module driving phloem development

Author

Wallner, Eva-Sophie, primary, Tonn, Nina, additional, Shi, Dongbo, additional, Luzzietti, Laura, additional, Wanke, Friederike, additional, Hunziker, Pascal, additional, Xu, Yingqiang, additional, Jung, Ilona, additional, Lopéz-Salmerón, Vadir, additional, Gebert, Michael, additional, Wenzl, Christian, additional, Lohmann, Jan U., additional, Harter, Klaus, additional, and Greb, Thomas, additional

Published
2023
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3. WUSCHEL acts as an auxin response rheostat to maintain apical stem cells in Arabidopsis

Author

Ma, Yanfei, primary, Miotk, Andrej, additional, Šutiković, Zoran, additional, Ermakova, Olga, additional, Wenzl, Christian, additional, Medzihradszky, Anna, additional, Gaillochet, Christophe, additional, Forner, Joachim, additional, Utan, Gözde, additional, Brackmann, Klaus, additional, Galván-Ampudia, Carlos S., additional, Vernoux, Teva, additional, Greb, Thomas, additional, and Lohmann, Jan U., additional

Published
2019
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5. Mobile PEAR transcription factors integrate positional cues to prime cambial growth

Author

Miyashima, Shunsuke, primary, Roszak, Pawel, additional, Sevilem, Iris, additional, Toyokura, Koichi, additional, Blob, Bernhard, additional, Heo, Jung-ok, additional, Mellor, Nathan, additional, Help-Rinta-Rahko, Hanna, additional, Otero, Sofia, additional, Smet, Wouter, additional, Boekschoten, Mark, additional, Hooiveld, Guido, additional, Hashimoto, Kayo, additional, Smetana, Ondřej, additional, Siligato, Riccardo, additional, Wallner, Eva-Sophie, additional, Mähönen, Ari Pekka, additional, Kondo, Yuki, additional, Melnyk, Charles W., additional, Greb, Thomas, additional, Nakajima, Keiji, additional, Sozzani, Rosangela, additional, Bishopp, Anthony, additional, De Rybel, Bert, additional, and Helariutta, Ykä, additional

Published
2019
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7. Translational control of phloem development by RNA G-quadruplex–JULGI determines plant sink strength

Author

Cho, Hyunwoo, primary, Cho, Hyun Seob, additional, Nam, Hoyoung, additional, Jo, Hunho, additional, Yoon, Joonseon, additional, Park, Chanyoung, additional, Dang, Tuong Vi T., additional, Kim, Eunah, additional, Jeong, Jongmin, additional, Park, Soyoung, additional, Wallner, Eva-Sophie, additional, Youn, Hyungjun, additional, Park, Jongmin, additional, Jeon, Jinseong, additional, Ryu, Hojin, additional, Greb, Thomas, additional, Choi, Kyuha, additional, Lee, Yoontae, additional, Jang, Sung Key, additional, Ban, Changill, additional, and Hwang, Ildoo, additional

Published
2018
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8. Spatial specificity of auxin responses coordinates wood formation

Author

Brackmann, Klaus, primary, Qi, Jiyan, additional, Gebert, Michael, additional, Jouannet, Virginie, additional, Schlamp, Theresa, additional, Grünwald, Karin, additional, Wallner, Eva-Sophie, additional, Novikova, Daria D., additional, Levitsky, Victor G., additional, Agustí, Javier, additional, Sanchez, Pablo, additional, Lohmann, Jan U., additional, and Greb, Thomas, additional

Published
2018
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10. A 3D gene expression atlas of the floral meristem based on spatial reconstruction of single nucleus RNA sequencing data

Author

Henrik Jönsson, Kerstin Kaufmann, Wenhao Yan, Neumann M, Xiaocai Xu, Nils Blüthgen, Thomas Greb, Julia Schumacher, Jan Traas, Cezary Smaczniak, Jose M. Muiño, Humboldt State University (HSU), Charité Center for Internal Medicine and Dermatology, Clinic for Psychosomatic Medicine, Charité-Universitätsmedizin Berlin, Berlin, Germany., Universität Heidelberg [Heidelberg] = Heidelberg University, University of Cambridge [UK] (CAM), Reproduction et développement des plantes (RDP), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Humboldt University Of Berlin, Neumann, Manuel [0000-0002-7883-0843], Xu, Xiaocai [0000-0002-2972-4251], Smaczniak, Cezary [0000-0002-4663-8275], Schumacher, Julia [0000-0001-6509-7315], Blüthgen, Nils [0000-0002-0171-7447], Greb, Thomas [0000-0002-6176-646X], Jönsson, Henrik [0000-0003-2340-588X], Traas, Jan [0000-0001-5107-1472], Kaufmann, Kerstin [0000-0001-7960-6256], Muino, Jose M [0000-0002-6403-7262], and Apollo - University of Cambridge Repository

Subjects
Plant molecular biology, Cell, Meristem, Gene Expression, General Physics and Astronomy, 570 Biologie, Computational biology, Flowers, Biology, General Biochemistry, Genetics and Molecular Biology, Transcriptome, Gene Expression Regulation, Plant, ddc:570, Gene expression, medicine, Plant Proteins, Shoot apical meristem, Multidisciplinary, Sequence Analysis, RNA, fungi, RNA, food and beverages, General Chemistry, Plant cell, Computational biology and bioinformatics, medicine.anatomical_structure, [SDE]Environmental Sciences, Developmental biology, Function (biology)
Abstract

Identity and functions of plant cells are influenced by their precise cellular location within the plant body. Cellular heterogeneity in growth and differentiation trajectories results in organ patterning. Therefore, assessing this heterogeneity at molecular scale is a major question in developmental biology. Single-cell transcriptomics (scRNA-seq) allows to characterize and quantify gene expression heterogeneity in developing organs at unprecedented resolution. However, the original physical location of the cell is lost during the scRNA-seq procedure. To recover the original location of cells is essential to link gene activity with cellular function and morphology. Here, we reconstruct genome-wide gene expression patterns of individual cells in a floral meristem by combining single-nuclei RNA-seq with 3D spatial reconstruction. By this, gene expression differences among meristematic domains giving rise to different tissue and organ types can be determined. As a proof of principle, the data are used to trace the initiation of vascular identity within the floral meristem. Our work demonstrates the power of spatially reconstructed single cell transcriptome atlases to understand plant morphogenesis. The floral meristem 3D gene expression atlas can be accessed at http://threed-flower-meristem.herokuapp.com

Published
2022
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