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Made available in DSpace on 2018-12-11T17:04:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-12-01 The Amazonian brown brocket deer (Mazama nemorivaga) is an endemic species of the Amazon rainforest region, which has suffered constant threats due to hunting and increasing deforestation. Artificial insemination (AI), combined with genomic banks, is considered an important tool for maintaining conservation programs of endangered species; however, the number of live offspring born from AI in non-domesticated cervids is extremely low. Thus, studies designed to develop, adapt, or enhance AI techniques are of fundamental importance. This report describes a successful transposition of the cervix with semen deposition in the uterine lumen of a M. nemorivaga female, based on the transcervical AI technique used in sheep, as well as using specific tools develop for IA in small ruminants, which have resulted in the birth of a healthy male fawn. Department of Preventive Veterinary Medicine and Animal Reproduction College of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP) Deer Research and Conservation Center (NUPECCE) Department of Animal Science FCAV-UNESP Department of Clinical and Veterinary Surgery FCAV-UNESP Department of Preventive Veterinary Medicine and Animal Reproduction College of Agricultural and Veterinary Sciences (FCAV) São Paulo State University (UNESP) Deer Research and Conservation Center (NUPECCE) Department of Animal Science FCAV-UNESP Department of Clinical and Veterinary Surgery FCAV-UNESP

Made available in DSpace on 2018-11-26T17:15:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 Graduate Program in Zoology of the Federal University of Parana Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) The ovarian cycle in howler monkeys (genus Alouatta) has beean investigated through several biological parameters (ranging between 16.3 and 29.5 days); however, no data exist concerning the ovarian activity of the southern brown howler monkey (Alouatta guariba clamitans). This study aimed to describe the ovarian cycle of A. g. clamitans by profiling fecal progestin concentrations. Over 20 weeks, fecal samples of eight captive adult females of A. g. clamitans were collected. The collections were made at dawn, 5 days a week, and the samples were frozen immediately following collection. Next, they were dried, pulverized and hormonal metabolites were extracted to determine progestin concentrations by enzyme immunoassay. Of the 758 samples tested, the mean concentration of fecal progestins was 2866.40 +/- 470.03 ng/g of dry feces, while the mean concentration at baseline was 814.47 +/- 164.36 ng/g of dry feces. Among the eight females, one showed no ovarian cyclicity and three presented periods of probable absence of cyclicity and low progestin concentrations. A mean duration of 16 +/- 0.52 days was observed for the 35 cycles studied. The interluteal phase lasted 4 +/- 0.37 days on average, with a mean concentration of fecal progestins of 467.98 +/- 29.12 ng/g of dry feces, while the luteal phase lasted 11 +/- 0.50 days, with a mean concentration of 4283.27 +/- 193.31 ng/g of dry feces. Besides describing the characteristics of the ovarian cycle, possible causes for the low concentrations of fecal progestins and periods of absence of cyclicity are also discussed. Univ Fed Parana, Dept Zool, Programa Posgrad Zool, Curitiba, Parana, Brazil Ctr Pesquisas Biol Indaial, Indaial, Brazil Univ Estadual Paulista, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos, Jaboticabal, Brazil Univ Reg Blumenau, Dept Ciencias Nat, Blumenau, Brazil Univ Estadual Paulista, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos, Jaboticabal, Brazil CNPq: 303757/2012-4 CNPq: 131196/2010-3

Made available in DSpace on 2014-12-03T13:11:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-03-04Bitstream added on 2014-12-03T13:22:36Z : No. of bitstreams: 1 WOS000334458900002.pdf: 1785302 bytes, checksum: 67376de2e4b2cb31a6e4a38aec22f56a (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Background: The red brocket deer, Mazama americana, has at least six distinct karyotypes in different regions of South America that suggest the existence of various species that are today all referred to as M. americana. From an evolutionary perspective, the red brockets are a relatively recent clade that has gone through intense diversification. This study sought to prove the existence of post-zygotic reproductive isolation in deer offspring between distinct chromosome lineages. To achieve this, inter-cytotype and intra-cytotype crosses were performed, which resulted in both F1 hybrid (n = 5) and pure offspring (n = 3) in captivity.Results: F1 females were analyzed in terms of their karyotypes, ovarian histology, estrous cycles and in vitro embryo production. Pure females presented parameters that were similar to those previously reported for M. Americana; however, the parameters for hybrid females were different. Two hybrids were determined to be sterile, while the remaining hybrids presented characteristics of subfertility.Conclusions: The results support the existence of well-established reproductive isolation among the most distant karyotype lineages and elucidates the need to define all karyotype variants and their geographical ranges in order to define the number of species of red brocket. Univ Estadual Paulista, UNESP, FCAV, Dept Zootecnia,NUPECCE Nucl Pesquisa & Conservaca, BR-14884900 Jaboticabal, SP, Brazil UNESP, FCAV, Programa Posgrad Med Vet Reproducao Anim, BR-14884900 Jaboticabal, SP, Brazil Fac Vet Med, Lab Embryol & Biotechn Reprod, BR-91501970 Porto Alegre, RS, Brazil Univ Estadual Paulista, UNESP, FCAV, Dept Zootecnia,NUPECCE Nucl Pesquisa & Conservaca, BR-14884900 Jaboticabal, SP, Brazil UNESP, FCAV, Programa Posgrad Med Vet Reproducao Anim, BR-14884900 Jaboticabal, SP, Brazil