3 results on '"Eun-Sang Cho"'
Search Results
2. Four-week inhalation toxicity study of 1-propanol in F344 rats
- Author
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Ka-Young Park, Cheol-Hong Lim, Eun-Sang Cho, and Yong-Soon Kim
- Subjects
medicine.medical_specialty ,No-observed-adverse-effect level ,Health, Toxicology and Mutagenesis ,F344 rats ,Physiology ,010501 environmental sciences ,Toxicology ,030226 pharmacology & pharmacy ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,Medicine ,0105 earth and related environmental sciences ,Toxicity data ,Hematology ,integumentary system ,Inhalation ,business.industry ,Blood biochemistry ,1-Propanol ,chemistry ,Toxicity ,Original Article ,business - Abstract
1-Propanol is used as a solvent for waxes, vegetable oils, resins, cellulose esters, and ethers, and is not considered harmful to humans by food and non-occupational exposures. However, workers are potentially exposed to 1-propanol by inhalation when it is used in the workplace. Thus, inhalation toxicity data are needed to assess the hazard of 1-propanol for workers safety. Five male and five female F344 rats were exposed to 1-propanol vapor for 4-weeks (6 h/day, 5 days/week) at concentrations of 0, 100, 400, and 1600 ppm in a whole-body inhalation chamber system. The actual exposure concentrations were 100.11 ± 5.10, 403.19 ± 12.31, and 1598.08 ± 139.58 ppm for the low, middle, and high dose groups, respectively. No clinical signs, significant mean body weight changes, significant changes of hematology or blood biochemistry results, or histopathological abnormalities were seen related to exposure to the test substance. Under the conditions of this study, the no-observed-adverse-effect level of 1-propanol was over 1600 ppm.
- Published
- 2020
3. Aqueous extract of Phragmitis rhizoma ameliorates myelotoxicity of docetaxel in vitro and in vivo
- Author
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Ok-Sun Bang, Eunna Huh, No Soo Kim, Eun-Sang Cho, You Jin Lee, Young Ah Kim, and Jinhee Kim
- Subjects
0301 basic medicine ,medicine.medical_treatment ,Docetaxel ,Pharmacology ,Myelotoxicity ,0302 clinical medicine ,Colony-Stimulating Factors ,Bone Marrow ,Phragmitis rhizoma ,Colony-forming unit ,Stem Cell Factor ,lcsh:Other systems of medicine ,General Medicine ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Toxicity ,Taxoids ,Research Article ,medicine.drug ,Coumaric Acids ,Intraperitoneal injection ,Antineoplastic Agents ,Bone Marrow Cells ,Enzyme-Linked Immunosorbent Assay ,Thymus Gland ,Poaceae ,Real-Time Polymerase Chain Reaction ,03 medical and health sciences ,In vivo ,Human Umbilical Vein Endothelial Cells ,medicine ,Animals ,Humans ,Chemotherapy ,Viability assay ,Adverse side effects ,Blood Cells ,Interleukin-6 ,Plant Extracts ,business.industry ,Fibroblasts ,Hematopoietic Stem Cells ,lcsh:RZ201-999 ,Hematopoiesis ,Mice, Inbred C57BL ,030104 developmental biology ,Complementary and alternative medicine ,Interleukin-3 ,Bone marrow ,Propionates ,business ,Rhizome ,Spleen - Abstract
Background A variety of anticancer chemotherapeutics induce adverse side effects including myelotoxicity. Dried roots of Phragmites communis Trinius, Phragmitis rhizoma, have been clinically used in traditional folk medicine to relieve various symptoms like fever. In this study, we evaluated the protective effect of the aqueous extract of Phragmitis rhizoma (EPR) against docetaxel-induced myelotoxicity in vitro and in vivo. Methods The in vitro myelo-protective effect of EPR was evaluated using the colony forming unit (CFU) assay with hematopoietic progenitor cells. The in vivo efficacy of EPR was evaluated in myelosuppressed C57BL/6 male mice which were induced by repeated intraperitoneal injections of 30 mg/kg docetaxel for 3 times. EPR was orally administered for 4 days to docetaxel-induced myelosuppressed C57BL/6 male mice which were induced by intraperitoneal injection of 30 mg/kg docetaxel for 3 times: Group 1 (vehicle control, n = 10), Group 2 (docetaxel plus vehicle, n = 10), Group 3 (docetaxel plus EPR 30 mg/kg, n = 10), Group 4 (docetaxel plus EPR 100 mg/kg, n = 10) and Group 5 (docetaxel plus EPR 300 mg/kg, n = 10). Whole blood counts were measured automatically, and immune organs were histologically examined. Expression of immunomodulatory cytokines was measured by quantitative real-time polymerase chain reaction or enzyme-linked immunosorbent assay. The toxicity of EPR itself was evaluated in normal human cell lines including IMR-90, foreskin fibroblast and human umbilical vein endothelial cells. The hepatotoxicity of EPR was predicted by multi-parametric assays involving cell viability, caspase 3/7 activity, GSH contents and LDH leakage using the HepaRG hepatic cell line. Results Co-treatment of EPR or its major component, p-hydroxycinnamic acid, increased the numbers of hematopoietic CFU counts in the docetaxel-induced in vitro myelotoxicity assay system. The in vitro protective effect of EPR against docetaxel toxicity was replicated in a myelosuppressed animal model: white blood cells, neutrophils, lymphocytes and red blood cells rebounded; bone marrow niche and structural integrity of the thymus were preserved; and the expression of immune-stimulating cytokines including IL3, IL6, SCF and GM-CSF was enhanced. Furthermore, EPR and p-hydroxycinnamic acid promoted the proliferation of primary splenocytes and thymocytes. In the toxicity assays, no remarkable signs related with toxicity were observed in all tested normal human cells and HepaRG. Conclusions EPR has the potential to ameliorate docetaxel-mediated myelotoxicity in both in vitro and in vivo models. However, the identification of the responsible active components and the precise underlying myelo-protective mechanism of EPR need to be elucidated before novel drug development using EPR can precede. Electronic supplementary material The online version of this article (doi:10.1186/s12906-017-1890-1) contains supplementary material, which is available to authorized users.
- Published
- 2017
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