1. Size prediction of recombinant human growth hormone nanoparticles produced by supercritical fluid precipitation
- Author
-
Dong-Hun Cho, Changhyun Lim, Dahlkyun Oh, and Dongjin Pyo
- Subjects
Chemistry, Pharmaceutical ,Biochemistry ,Analytical Chemistry ,Chemical Precipitation ,Humans ,Particle Size ,Aqueous solution ,Supercritical carbon dioxide ,Chromatography ,Ethanol ,Human Growth Hormone ,Precipitation (chemistry) ,Chemistry ,Water ,Carbon Dioxide ,Recombinant Proteins ,Supercritical fluid ,Solutions ,Solubility ,Chemical engineering ,Particle-size distribution ,Solvents ,Nanoparticles ,Particle ,Particle size ,Dispersion (chemistry) - Abstract
Solution-enhanced dispersion by supercritical fluids (SEDS) was applied to produce nano-sized recombinant human growth hormone (hGH) particles. Ethanol was used to help the supercritical carbon dioxide to extract water from the aqueous protein solution. Various sizes of hGH nanoparticles were successfully prepared with a narrow particle size distribution from aqueous ethanol solution without using any additive. The theoretical particle sizes were deduced from the calculated droplet sizes based on a modified Jasuja's equation. The calculated mean particle sizes and the experimentally obtained ones were compared and the results showed an excellent correlation coefficient (R (2)) of 0.995.
- Published
- 2006
- Full Text
- View/download PDF