1. Evaluation of AFLP for the grouping of Bradyrhizobium strains
- Author
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Philippe de Lajudie, Anne Willems, Monique Gillis, and F Doignon-Bourcier
- Subjects
IV — Inventaire, Caractérisation et Suivi de la Diversité Microbienne / Inventory, Characterization and Monitoring of Microbial Diversity ,ADN ,DENDROGRAMME ,BACTERIE ,TAXONOMIE ,PHENOTYPE ,TECHNIQUE AFLP ,Bradyrhizobium ,Numerical taxonomy ,Intergenic region ,TECHNIQUE PCR ,ETUDE COMPARATIVE ,Genetics ,Gene ,Ecology, Evolution, Behavior and Systematics ,ANALYSE NUMERIQUE ,biology ,General Medicine ,biology.organism_classification ,16S ribosomal RNA ,HYBRIDATION ,ETUDE EXPERIMENTALE ,Animal Science and Zoology ,Amplified fragment length polymorphism ,Taxonomy (biology) ,Restriction fragment length polymorphism - Abstract
In recent years we have characterized 250 Bradyrhizobium strains, mainly from Senegal, using several taxonomic techniques i.e. numerical taxonomy of pheno-typic features, Biolog system, SDS-PAGE of total cellular proteins, 16S rDNA RFLP and sequence analyses, 16S-23S rDNA intergenic gene spacer RFLP and sequence analyses, AFLP, DNA:DNA hybridizations. Here we evaluate the taxonomic resolving power of these techniques by comparing the results obtained on various subsets of the same strains. We conclude that AFLP is a useful method for an initial grouping of Bradyrhizobium strains and provides infraspecific information. However, from a limited comparison, it appears that the less labor-intensive 16S-23S rDNA intergenic gene spacer analysis gives similar results and may provide additional information on deeper groupings.
- Published
- 2001