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We examine the role of working-time mismatches (defined as the difference between actual and desired weekly working hours) in academics’ job satisfaction. In doing so, we investigate how academics’ career stage (predoc vs. postdoc) and contract status (part-time vs. full-time employment) moderate the relation between a mismatch in working hours and job-related well-being. Our results are based on longitudinal survey data among junior academics in the STEM fields indicate that mismatches in working hours are prevalent in academia and are associated with a loss of job satisfaction. In this context, overemployment (working more than desired) tends to unfold more severe consequences with respect to job satisfaction compared to underemployment. We find evidence for a similar reaction of doctoral students and postdocs with respect to job satisfaction when experiencing a working-time mismatch. Part-time employment positively moderates the link between working-time mismatch and job satisfaction for the case of underemployment.

Schmalenbachs Zeitschrift f��r betriebswirtschaftliche Forschung 74(January), 26 (2022). doi:10.1007/s41471-021-00129-1, Published by Westdt. Verl., K��ln ; Opladen

Background Newcastle disease is a devastating disease in poultry caused by virulent Newcastle disease virus (NDV), a paramyxovirus endemic in many regions of the world despite intensive vaccination. Phylogenetic analyses reveal ongoing evolution of the predominant circulating genotype 2.VII, and the relevance of potential antigenic drift is under discussion. To investigate variation within neutralization-sensitive epitopes within the protein responsible for receptor binding, i.e. the Hemagglutinin-Neuraminidase (HN) spike protein, we were interested in establishing genotype-specific monoclonal antibodies (MAbs). Methods An HN-enriched fraction of a gradient-purified NDV genotype 2.VII was prepared and successfully employed to induce antibodies in BalbC mice that recognize conformationally intact sites reactive by haemagglutination inhibition (HI). For subsequent screening of mouse hybridoma cultures, an NDV-ELISA was established that utilizes Concanavalin A (ConA-ELISA) coupled glycoproteins proven to present conformation-dependent epitopes. Results Six out of nine selected MAbs were able to block receptor binding as demonstrated by HI activity. One MAb recognized an epitope only present in the homologue virus, while four other MAbs showed weak reactivity to selected other genotypes. On the other hand, one broadly cross-reacting MAb reacted with all genotypes tested and resembled the reactivity profile of genotype-specific polyclonal antibody preparations that point to minor antigenic differences between tested NDV genotpyes. Conclusions These results point to the concurrent presence of variable and conserved epitopes within the HN molecule of NDV. The described protocol should help to generate MAbs against a variety of NDV strains and to enable in depth analysis of the antigenic profiles of different genotypes.

The intention of “doing good for society” is regarded to be a crucial motivator for employees in the public sector in order for them to perform well. Recent research in the public sector literature calls for a deeper understanding of how this specific public service motivation (PSM) is shaped. In our paper, we analyze how different degrees of inclusion in the public sector matter for PSM. We investigate how prospects of employment relations (fixed-term versus permanent contracts) and temporal differences (part-time versus full-time employment) moderate PSM in the public and in the private service. Our findings show that aspects of PSM are related by these employment characteristics in various ways, suggesting that the factors influencing PSM are multifaceted and that actual employment conditions need to be taken into consideration when assessing PSM.

Aquatic birds can act as vectors and reservoir hosts for pathogens relevant for wild birds and poultry as well as human health. In this study, we address the questions (1) if the Egyptian goose (Alopochen aegyptiacus), as one of the most successful neozootic bird species in Europe, carry infectious agents that are relevant for poultry and wild birds and (2) if seasonal prevalences of these infectious agents differ from those of native geese species. In 2015 and 2016, up to 190 Egyptian geese from Western Germany were investigated serologically for antibodies (Ab) against influenza A viruses (IAV), Avian avulavirus 1 (AAvV-1), aviadenoviruses, Duck atadenovirus A (syn.: egg drop syndrome 1976 virus) (EDSV), and West Nile virus (WNV). Ab were detected against IAV in 6.1% (10/164), against AAvV-1 in 2.4% (4/165), against EDSV in 15.2% (16/105), and against aviadenoviruses in 0.86% (1/116) of the geese blood samples, respectively. None of the birds had Ab against WNV (0/84). PCR-based techniques (cloacal and/or pharyngeal swabs) were applied for the presence of IAV, AAvV-1, Mycoplasma spp., and Riemerella anatipestifer. Riemerella DNA was detected in the pharyngeal swabs with an overall prevalence of 70.3% (104/148). Neither Mycoplasma DNA nor IAV or AAvV-1 RNA could be detected in the pharynx or cloaca of the examined birds. Our study shows that Egyptian geese are frequent carriers of Riemerella anatipestifer and furthermore provides serological evidence of exposure to IAV, AAvV-1, and EDSV. It is one of very few studies on infectious agents of neozootic bird species. Comparing our results from a neozootic non-migratory goose species with published results from native migratory geese species (bean goose (Anser fabalis) and white-fronted goose (A. albifrons)) and another neozootic non-migratory goose species (Canada goose (Branta canadensis)), we found differences in the seroprevalence of viral pathogens. Native goose species show higher seroprevalences of IAV and AAvV-1, whereas neozootic non-migratory geese reveal higher seroprevalences for EDSV. The findings are discussed in the frame of seasonal variations in selected infectious agents, differences in sampling periods, and contrasting movement ecology of the different geese species.

Newcastle disease viruses (NDV) isolated from pigeons (pigeon paramyxovirus type 1; PPMV-1) are mostly of mesogenic pathotype and characterized by a polybasic amino acid sequence motif at the fusion protein (F) cleavage site. This feature also applies to isolate R75/98 from Germany. Its genome consists of 15,192 nucleotides and it specifies an intracerebral pathogenicity index (ICPI) of 1.1, as is typical for mesogenic NDV. Recombinant R75/98 (rR75/98) derived by reverse genetics also possesses a polybasic F protein cleavage site but exhibits ICPI of 0.28, indicating a lentogenic virus. While ten virus passages of rR75/98 on embryonated chicken eggs did not result in any alteration of virus characteristics, virus which had been re-isolated from the brain of an intracerebrally inoculated chicken showed an increase in virulence, characterized by an ICPI of 0.93. Comparison of whole genome sequences of rR75/98 and re-isolated rR75/98 (RrR75/98) demonstrated only two amino acid differences, one in the F protein (N472 K) and one in the polymerase protein (K2168R). This result indicates that only very few amino acid alterations are sufficient to modulate virus virulence in the presence of a polybasic amino acid sequence at the proteolytic F protein cleavage site.

Making use of unique balanced panel data for the German chemical sector from the years 2008 to 2011, we explore the extent to which managers' compensation was affected by the economic crisis and the extent to which it increased afterwards. Carrying out longitudinal analyses, we find that, on average, bonus payments (in contrast to fixed salaries) decrease considerably during the crisis. The economic upturn in 2011 then leads to an average increase in variable payments and total compensation to even above the pre-crisis level. Changes in bonus payments are negatively correlated over time. We find considerable differences across employees with respect to changes in bonus payments. Fixed salary changes are much more homogeneous over the period of crisis. We explore determinants of compensation changes and find that changes in compensation have a strong relationship with employees' age, firm size and hierarchical level. Our findings hint at the relevance of an incentive perspective. We also examine that certain parts of managers seem to have more power to influence their compensation than others. Inequality in managers' compensation decreased during the crisis.

Using data on executive compensation for the German chemical industry, we investigate the relevance of two theoretical approaches that focus on bonuses as part of a long term wage policy of a firm. The first approach argues that explicit bonuses serve as substitutes for implicit career concerns. The second approach claims that bonuses are used as complements to an executive's internal career. Our data show that bonus payments are mostly prevalent among senior executives at higher hierarchy levels and rather for management jobs than for jobs in research and development. This is true for the whole chemical sector as well as for single large corporations. The findings indicate that the two theoretical views are not mutually exclusive, but are both relevant in practice.

This contribution investigates severance payments for dismissed employees in Germany. Particularly, it responds to two questions: “Who receives severance payments?” and “By which characteristics is the level of severance payments determined?”. Individual and collective dismissals are analyzed separately. This is the first study on this issue using individual representative data—the German Socio-Economic Panel—and multivariate methods. The results indicate that rather women, persons with many years of tenure and working in large firms receive severance payments. There is a huge variance in the size of the payments. The most important determinants are the previous wage, tenure and age.

Das Internet hat die Moglichkeiten der Mitarbeiterrekrutierung deutlich erweitert. In diesem Beitrag wird zunachst eine Marktbeschreibung vorgenommen. Anschliesend werden Vorteile und mogliche Probleme einer Rekrutierung neuer Mitarbeiter uber das Internet diskutiert. Die empirische Untersuchung dieses Beitrags setzt sich mit den Fragen auseinander, welche Personen uber das Internet nach Stellen suchen bzw. neue Arbeitsplatze finden und welche Konsequenzen sich fur die entsprechenden Arbeitnehmer ergeben. Die Ergebnisse deuten darauf hin, dass eher gut ausgebildete Personen uber das Internet eine neue Stelle mit einer relativ langen wochentlichen Arbeitszeit finden. Bei Kontrolle verschiedener individueller und arbeitsplatzspezifischer Charakteristika sind keine Unterschiede im Lohn und der Arbeitszufriedenheit im Vergleich zu anderen Wegen der Stellenfindung festzustellen.

Vor nunmehr 10 Jahren wurde das Vermittlungsmonopol der Bundesagentur fur Arbeit abgeschafft. Seitdem ist die Vermittlung von Arbeitnehmern an Unternehmen auch fur private Agenturen moglich. In diesem Beitrag werden auf Grundlage suchund signaltheoretischer Uberlegungen mogliche Selektionseffekte durch private Arbeitsvermittler und deren Vermittlungserfolg im Vergleich zur Bundesagentur fur Arbeit empirisch untersucht. Es zeigt sich, dass Selektionseffekte in der Form Relevanz besitzen, dass sich sowohl die vermittelten Personen als auch die Jobs deutlich voneinander unterscheiden. Selbst unter Kontrolle etlicher personen- und jobspezifischer Merkmale sind positive Vermittlungsdifferentiale in Form hoherer Lohne bei Vermittlungen uber private Agenturen zu beobachten. Die entsprechenden Personen verfugten aber auch bereits auf ihrer letzten Arbeitstelle uber hohere Lohne.

Background Protection against infection by Newcastle disease virus (NDV), also designated as avian paramyxovirus subtype-1 (APMV-1), is mediated by immune responses to the two surface glycoproteins, hemagglutinin-neuraminidase (HN) and fusion (F) protein. Thus, a chimeric APMV-1 based vaccine that encodes APMV-8 HN- and F-proteins and expresses the hemagglutinin of avian influenza virus (AIV) H5N1, is able to protect against HPAIV H5N1 but fails to protect against NDV [PLoS One8:e72530, 2013]. However, it is unclear whether avirulent APMV-subtypes, like APMV-8 can induce subtype-specific immunity and protect from a homologous challenge. Findings APMV-8 infections of 3- and 6-weeks-old specific pathogen free (SPF)-chickens did not induce any clinical signs but was associated with virus shedding for up to 6 days. Viral replication was only detected in oropharyngeal- and never in cloacal swabs. Upon reinfection with homologous APMV-8, viral shedding was restricted to day 2 and in contrast to naive SPF-chickens, only RNA but no infectious virus was recovered. No protection was induced against virulent NDV challenge, although morbidity and mortality was delayed in APMV-8 primed chickens. This lack of protection is in line with a lack of reactivity of APMV-8 specific sera to APMV-1 HN-protein: Neither by hemagglutin-inhibition (HI) test nor immunoblot analyses, cross-reactivity was detected, despite reactivity to internal proteins. Conclusions Immune responses mounted during asymptomatic APMV-8 infection limit secondary infection against homologues reinfection and facilitates a delay in the onset of disease in a subtype independent manner but is unable to protect against Newcastle disease, a heterologous APMV-subtype. Electronic supplementary material The online version of this article (doi:10.1186/1743-422X-11-179) contains supplementary material, which is available to authorized users.

The theory of compensating wage differentials is generally accepted. It states that firms have to pay wage bonuses for hazardous work. However, there is as yet no strong or even contrary evidence for compensating wage differentials in Germany. By estimating wage regressions with data from the German Socio-Economic Panel (GSOEP) and using individually perceived hazards of work accidents as a risk variable, evidence that firms do pay risk premiums for hazardous work are found even though other effects could dilute the existing wage bonuses. Taking into account these results, the incentives for German firms to invest in accident prevention are discussed in the context of the existing institutional conditions.

A consensus primer PCR approach was used to (i) investigate the presence of herpesviruses in wild and zoo equids (zebra, wild ass, tapir) and to (ii) study the genetic relationship of the herpesvirus of pigeons (columbid herpesvirus 1) to other herpesvirus species. The PCR assay, based on degenerate primers targeting highly conserved regions of the DNA polymerase gene of herpesviruses, was modified by using a mixture of degenerate and deoxyinosine-substituted primers. The applicability of the modification was validated by amplification of published DNA polymerase genes of 16 herpesvirus species and of the previously uncharacterized DNA polymerase genes of equine herpesvirus 3 (EHV-3) and equine herpesvirus 5 (EHV-5). The modified assay was then used for partial amplification of the polymerase of columbid herpesvirus 1 which is presently classified as a β-herpesvirus based on biological criteria. Sequence analysis of amplicons obtained from four different viral strains revealed a close relationship of columbid herpesvirus 1 to members of the subfamily Alphaherpesvirinae, especially to Marek’s disease herpesvirus. This was confirmed by characterization of additional 1.6 kb of the columbid herpesvirus 1 polymerase. Consensus PCR analysis of blood samples from zebras, a wild ass and a tapir revealed amplicons showing high percentages (>50%) of sequence identity to DNA polymerases of γ-herpesviruses. In particular, the zebra and the wild ass sequence were closely related to each other and to the polymerases of the equine γ-herpesviruses EHV-2 and EHV-5 with sequence identities of >80%. This is a first indication that novel γ-herpesviruses are present in wild and zoo equids.

Background Serological investigations of swine influenza virus infections and epidemiological conclusions thereof are challenging due to the complex and regionally variable pattern of co-circulating viral subtypes and lineages and varying vaccination regimes. Detection of subtype-specific antibodies currently depends on hemagglutination inhibition (HI) assays which are difficult to standardize and unsuitable for large scale investigations. Methods The nucleocapsid protein (NP) and HA1 fragments of the hemagglutinin protein (HA) of five different lineages (H1N1av, H1N1pdm, H1pdmN2, H1N2, H3N2) of swine influenza viruses were bacterially expressed and used as diagnostic antigens in indirect ELISA. Results Proteins were co-translationally mono-biotinylated and refolded in vitro into an antigenically authentic conformation. Western blotting and indirect ELISA revealed highly subtype-specific antigenic characteristics of the recombinant HA1 proteins although some cross reactivity especially among antigens of the H1 subtype were evident. Discrimination of antibodies directed against four swine influenza virus subtypes co-circulating in Germany was feasible using the indirect ELISA format. Conclusions Bacterially expressed recombinant NP and HA1 swine influenza virus proteins served as antigens in indirect ELISAs and provided an alternative to commercial blocking NP ELISA and HI assays concerning generic (NP-specific) and HA subtype-specific sero-diagnostics, respectively, on a herd basis.

Background The endemic status of highly pathogenic avian influenza virus (HPAIV) of subtype H5N1 in Egypt continues to devastate the local poultry industry and poses a permanent threat for human health. Several genetically and antigenically distinct H5N1 lineages co-circulate in Egypt: Strains of clade 2.2.1 proper replicate mainly in backyard birds causing the bulk of human infections, while a variant lineage within 2.2.1 (2.2.1v) appears to be perpetuated mainly in commercial poultry farms in Egypt. Viruses of the 2.2.1v lineage represent drift variants escaping from conventional vaccine-induced immunity and some of these strains also escaped detection by commercial real time reverse transcriptase PCR (RT-qPCR) protocols due to mismatches in the primers/probe binding sites. Results We developed therefore a versatile, sensitive and lineage-specific multiplex RT-qPCR for detection and typing of H5N1 viruses in Egypt. Analytical characterization was carried out using 50 Egyptian HPAIV H5N1 strains isolated since 2006 and 45 other avian influenza viruses (AIV). A detection limit of 400 cRNA copies per ml sample matrix was found. Higher diagnostic sensitivity of the multiplex assay in comparison to other generic H5 or M-gene based RT-qPCR assays were found by examination of 63 swab samples from experimentally infected chickens and 50 AIV-positive swab samples from different host species in the field in Egypt. Conclusions The new multiplex RT-qPCR assay could be useful for rapid high-throughput monitoring for the presence of HPAIV H5N1 in commercial poultry in Egypt. It may also aid in prospective epidemiological studies to further delineate and better control spread of HPAIV H5N1 in Egypt.