Search

Your search keyword '"Cellerino, A."' showing total 65 results
Start Over Author "Cellerino, A." Publisher springer science and business media llc
65 results on '"Cellerino, A."'

1. Goodbye Hartmann trial: a prospective, international, multicenter, observational study on the current use of a surgical procedure developed a century ago

Author

Perrone, Gennaro, primary, Giuffrida, Mario, additional, Abu-Zidan, Fikri, additional, Kruger, Vitor F., additional, Livrini, Marco, additional, Petracca, Gabriele Luciano, additional, Rossi, Giorgio, additional, Tarasconi, Antonio, additional, Tian, Brian W. C. A., additional, Bonati, Elena, additional, Mentz, Ricardo, additional, Mazzini, Federico N., additional, Campana, Juan P., additional, Gasser, Elisabeth, additional, Kafka-Ritsch, Reinhold, additional, Felsenreich, Daniel M., additional, Dawoud, Christopher, additional, Riss, Stefan, additional, Gomes, Carlos Augusto, additional, Gomes, Felipe Couto, additional, Gonzaga, Ricardo Alessandro Teixeira, additional, Canton, Cassio Alfred Brattig, additional, Pereira, Bruno Monteiro, additional, Fraga, Gustavo P., additional, Zem, Leticia Gonçalves, additional, Cordeiro-Fonseca, Vinicius, additional, de Mesquita Tauil, Renato, additional, Atanasov, Boyko, additional, Belev, Nikolay, additional, Kovachev, Nikola, additional, Meléndez, L. Juan José, additional, Dimova, Ana, additional, Dimov, Stefan, additional, Zelić, Zdravko, additional, Augustin, Goran, additional, Bogdanić, Branko, additional, Morić, Trpimir, additional, Chouillard, Elie, additional, Bajul, Melinda, additional, De Simone, Belinda, additional, Panis, Yves, additional, Esposito, Francesco, additional, Notarnicola, Margherita, additional, Lauka, Lelde, additional, Fabbri, Anna, additional, Hentati, Hassen, additional, Fnaiech, Iskander, additional, Aurélien, Venara, additional, Bougard, Marie, additional, Roulet, Maxime, additional, Demetrashvili, Zaza, additional, Pipia, Irakli, additional, Merabishvili, Giorgi, additional, Bouliaris, Konstantinos, additional, Koukoulis, Georgios, additional, Doudakmanis, Christos, additional, Xenaki, Sofia, additional, Chrysos, Emmanuel, additional, Kokkinakis, Stamatios, additional, Vassiliu, Panteleimon, additional, Michalopoulos, Nikolaos, additional, Margaris, Ioannis, additional, Kechagias, Aristotelis, additional, Avgerinos, Konstantinos, additional, Katunin, Jevgeni, additional, Lostoridis, Eftychios, additional, Nagorni, Eleni-Aikaterini, additional, Pujante, Antonio, additional, Mulita, Francesk, additional, Maroulis, Ioannis, additional, Vailas, Michail, additional, Marinis, Athanasios, additional, Siannis, Ioannis, additional, Bourbouteli, Eirini, additional, Manatakis, Dimitrios K., additional, Tasis, Nikolaos, additional, Acheimastos, Vasileios, additional, Maria, Sotiropoulou, additional, Stylianos, Kapiris, additional, Kuzeridis, Harilaos, additional, Korkolis, Dimitrios, additional, Fradelos, Evangelos, additional, Kavalieratos, George, additional, Petropoulou, Thalia, additional, Polydorou, Andreas, additional, Papacostantinou, Ioannis, additional, Triantafyllou, Tania, additional, Kimpizi, Despina, additional, Theodorou, Dimitrios, additional, Toutouzas, Konstantinos, additional, Chamzin, Alexandros, additional, Frountzas, Maximos, additional, Schizas, Dimitrios, additional, Karavokyros, Ioannis, additional, Syllaios, Athanasios, additional, Charalabopoulos, Alexandros, additional, Boura, Maria, additional, Baili, Efstratia, additional, Ioannidis, Orestis, additional, Loutzidou, Lydia, additional, Anestiadou, Elissavet, additional, Tsouknidas, Ioannis, additional, Petrakis, Georgios, additional, Polenta, Eleni, additional, Bains, Lovenish, additional, Gupta, Rahul, additional, Singh, Sudhir K., additional, Khanduri, Archana, additional, Bala, Miklosh, additional, Kedar, Asaf, additional, Pisano, Marcello, additional, Podda, Mauro, additional, Pisanu, Adolfo, additional, Martines, Gennaro, additional, Trigiante, Giuseppe, additional, Lantone, Giuliano, additional, Agrusa, Antonino, additional, Di Buono, Giuseppe, additional, Buscemi, Salvatore, additional, Veroux, Massimiliano, additional, Gioco, Rossella, additional, Veroux, Gastone, additional, Oragano, Luigi, additional, Zonta, Sandro, additional, Lovisetto, Federico, additional, Feo, Carlo V., additional, Pesce, Antonio, additional, Fabbri, Nicolò, additional, Lantone, Giulio, additional, Marino, Fabio, additional, Perrone, Fabrizio, additional, Vincenti, Leonardo, additional, Papagni, Vincenzo, additional, Picciariello, Arcangelo, additional, Rossi, Stefano, additional, Picardi, Biagio, additional, Del Monte, Simone Rossi, additional, Visconti, Diego, additional, Osella, Giulia, additional, Petruzzelli, Luca, additional, Pignata, Giusto, additional, Andreuccetti, Jacopo, additional, D’Alessio, Rossella, additional, Buonfantino, Massimo, additional, Guaitoli, Eleonora, additional, Spinelli, Stefano, additional, Sampietro, Gianluca Matteo, additional, Corbellini, Carlo, additional, Lorusso, Leonardo, additional, Frontali, Alice, additional, Pezzoli, Isabella, additional, Bonomi, Alessandro, additional, Chierici, Andrea, additional, Cotsoglou, Christian, additional, Manca, Giuseppe, additional, Delvecchio, Antonella, additional, Musa, Nicola, additional, Casati, Massimiliano, additional, Letizia, Laface, additional, Abate, Emmanuele, additional, Ercolani, Giorgio, additional, D’Acapito, Fabrizio, additional, Solaini, Leonardo, additional, Guercioni, Gianluca, additional, Cicconi, Simone, additional, Sasia, Diego, additional, Borghi, Felice, additional, Giraudo, Giorgio, additional, Sena, Giuseppe, additional, Castaldo, Pasquale, additional, Cardamone, Eugenia, additional, Portale, Giuseppe, additional, Zuin, Matteo, additional, Spolverato, Ylenia, additional, Esposito, Marialusia, additional, Isernia, Roberta Maria, additional, Di Salvo, Maria, additional, Manunza, Romina, additional, Esposito, Giuseppe, additional, Agus, Marcello, additional, Asti, Emanuele Luigi Giuseppe, additional, Bernardi, Daniele Tiziano, additional, Tonucci, Tommaso Panici, additional, Luppi, Davide, additional, Casadei, Massimiliano, additional, Bonilauri, Stefano, additional, Pezzolla, Angela, additional, Panebianco, Annunziata, additional, Laforgia, Rita, additional, De Luca, Maurizio, additional, Zese, Monica, additional, Parini, Dario, additional, Jovine, Elio, additional, De Sario, Giuseppina, additional, Lombardi, Raffaele, additional, Aprea, Giovanni, additional, Palomba, Giuseppe, additional, Capuano, Marianna, additional, Argenio, Giulio, additional, Orio, Gianluca, additional, Armellino, Mariano Fortunato, additional, Troian, Marina, additional, Guerra, Martina, additional, Nagliati, Carlo, additional, Biloslavo, Alan, additional, Germani, Paola, additional, Aizza, Giada, additional, Monsellato, Igor, additional, Chahrour, Ali Chaouki, additional, Anania, Gabriele, additional, Bombardini, Cristina, additional, Bagolini, Francesco, additional, Sganga, Gabriele, additional, Fransvea, Pietro, additional, Bianchi, Valentina, additional, Boati, Paolo, additional, Ferrara, Francesco, additional, Palmieri, Francesco, additional, Cianci, Pasquale, additional, Gattulli, Domenico, additional, Restini, Enrico, additional, Cillara, Nicola, additional, Cannavera, Alessandro, additional, Nita, Gabriela Elisa, additional, Sarnari, Jlenia, additional, Roscio, Francesco, additional, Clerici, Federico, additional, Scandroglio, Ildo, additional, Berti, Stefano, additional, Cadeo, Alessandro, additional, Filippelli, Alice, additional, Conti, Luigi, additional, Grassi, Carmine, additional, Cattaneo, Gaetano Maria, additional, Pighin, Marina, additional, Papis, Davide, additional, Gambino, Giovanni, additional, Bertino, Vanessa, additional, Schifano, Domenico, additional, Prando, Daniela, additional, Fogato, Luisella, additional, Cavallo, Fabio, additional, Ansaloni, Luca, additional, Picheo, Roberto, additional, Pontarolo, Nicholas, additional, Depalma, Norma, additional, Spampinato, Marcello, additional, D’Ugo, Stefano, additional, Lepre, Luca, additional, Capponi, Michela Giulii, additional, Campa, Rossella Domenica, additional, Sarro, Giuliano, additional, Dinuzzi, Vincenza Paola, additional, Olmi, Stefano, additional, Uccelli, Matteo, additional, Ferrari, Davide, additional, Inama, Marco, additional, Moretto, Gianluigi, additional, Fontana, Michele, additional, Favi, Francesco, additional, Picariello, Erika, additional, Rampini, Alessia, additional, Barberis, Andrea, additional, Azzinnaro, Antonio, additional, Oliva, Alba, additional, Totaro, Luigi, additional, Benzoni, Ilaria, additional, Ranieri, Valerio, additional, Capolupo, Gabriella Teresa, additional, Carannante, Filippo, additional, Caricato, Marco, additional, Ronconi, Maurizio, additional, Casiraghi, Silvia, additional, Casole, Giovanni, additional, Pantalone, Desire, additional, Alemanno, Giovanni, additional, Scheiterle, Maximilian, additional, Ceresoli, Marco, additional, Cereda, Marco, additional, Fumagalli, Chiara, additional, Zanzi, Federico, additional, Bolzon, Stefano, additional, Guerra, Enrico, additional, Lecchi, Francesca, additional, Cellerino, Paola, additional, Ardito, Antonella, additional, Scaramuzzo, Rosa, additional, Balla, Andrea, additional, Lepiane, Pasquale, additional, Tartaglia, Nicola, additional, Ambrosi, Antonio, additional, Pavone, Giovanna, additional, Palini, Gian Marco, additional, Veneroni, Simone, additional, Garulli, Gianluca, additional, Ricci, Claudio, additional, Torre, Beatrice, additional, Russo, Iris Shari, additional, Rottoli, Matteo, additional, Tanzanu, Marta, additional, Belvedere, Angela, additional, Milone, Marco, additional, Manigrasso, Michele, additional, De Palma, Giovanni Domenico, additional, Piccoli, Micaela, additional, Pattacini, Gianmaria Casoni, additional, Magnone, Stefano, additional, Bertoli, Paolo, additional, Pisano, Michele, additional, Massucco, Paolo, additional, Palisi, Marco, additional, Luzzi, Andrea-Pierre, additional, Fleres, Francesco, additional, Clarizia, Guglielmo, additional, Spolini, Alessandro, additional, Kobe, Yoshiro, additional, Toma, Takayuki, additional, Shimamura, Fumihiko, additional, Parker, Robert, additional, Ranketi, Sinkeet, additional, Mitei, Mercy, additional, Svagzdys, Saulius, additional, Pauzas, Henrikas, additional, Zilinskas, Justas, additional, Poskus, Tomas, additional, Kryzauskas, Marius, additional, Jakubauskas, Matas, additional, Zakaria, Andee Dzulkarnaen, additional, Zakaria, Zaidi, additional, Wong, Michael Pak-Kai, additional, Jusoh, Asri Che, additional, Zakaria, Muhammad Nazreen, additional, Cruz, Daniel Rios, additional, Elizalde, Aurea Barbara Rodriguez, additional, Reynaud, Alejandro Bañon, additional, Hernandez, Edgard Efren Lozada, additional, Monroy, Jose maria Victor Palomo, additional, Hinojosa-Ugarte, Diego, additional, Quiodettis, Martha, additional, Du Bois, María Esther, additional, Latorraca, José, additional, Major, Piotr, additional, Pędziwiatr, Michał, additional, Pisarska-Adamczyk, Magdalena, additional, Walędziak, Maciej, additional, Kwiatkowski, Andrzej, additional, Czyżykowski, Łukasz, additional, da Costa, Silvia Dantas, additional, Pereira, Bela, additional, Ferreira, Ana Rita Oliveira, additional, Almeida, Filipe, additional, Rocha, Ricardo, additional, Carneiro, Carla, additional, Perez, Diego Pita, additional, Carvas, João, additional, Rocha, Catarina, additional, Ferreira, Cátia, additional, Marques, Rita, additional, Fernandes, Urânia, additional, Leao, Pedro, additional, Goulart, André, additional, Pereira, Rita Gonçalves, additional, Patrocínio, Sara Daniela Direito, additional, de Mendonça, Nuno Gonçalo Gonçalves, additional, Manso, Maria Isabel Cerqueira, additional, Morais, Henrique Manuel Cardoso, additional, Cardoso, Paulo Sebastião, additional, Calu, Valentin, additional, Miron, Adrian, additional, Toma, Elena Adelina, additional, Gachabayov, Mahir, additional, Abdullaev, Abakar, additional, Litvin, Andrey, additional, Nechay, Taras, additional, Tyagunov, Alexander, additional, Yuldashev, Anvar, additional, Bradley, Alison, additional, Wilson, Michael, additional, Panyko, Arpád, additional, Látečková, Zuzana, additional, Lacko, Vladimír, additional, Lesko, Dusan, additional, Soltes, Marek, additional, Radonak, Jozef, additional, Turrado-Rodriguez, Victor, additional, Termes-Serra, Roser, additional, Morales-Sevillano, Xavier, additional, Lapolla, Pierfrancesco, additional, Mingoli, Andrea, additional, Brachini, Gioia, additional, Degiuli, Maurizio, additional, Sofia, Silvia, additional, Reddavid, Rossella, additional, de Manzoni Garberini, Andrea, additional, Buffone, Angelica, additional, del Pozo, Eduardo Perea, additional, Aparicio-Sánchez, Daniel, additional, Dos Barbeito, Sandra, additional, Estaire-Gómez, Mercedes, additional, Vitón-Herrero, Rebeca, additional, de los Ángeles Gil Olarte-Marquez, Mª, additional, Gil-Martínez, José, additional, Alconchel, Felipe, additional, Nicolás-López, Tatiana, additional, Rahy-Martin, Aida Cristina, additional, Pelloni, María, additional, Bañolas-Suarez, Raquel, additional, Mendoza-Moreno, Fernando, additional, Nisa, Francisca García-Moreno, additional, Díez-Alonso, Manuel, additional, Rodas, María Elisa Valle, additional, Agundez, María Carmona, additional, Andrés, María Inmaculada Pérez, additional, Moreira, Claudia Cristina Lopes, additional, Perez, Aintzane Lizarazu, additional, Ponce, Iñigo Augusto, additional, González-Castillo, Ana María, additional, Membrilla-Fernández, Estela, additional, Salvans, Silvia, additional, Serradilla-Martín, Mario, additional, Pardo, Pablo Sancho, additional, Rivera-Alonso, Daniel, additional, Dziakova, Jana, additional, Huguet, Jose Mugüerza, additional, Valle, Naila Pagès, additional, Ruiz, Enrique Colás, additional, Valcárcel, Cristina Rey, additional, Moreno, Cristina Ruiz, additional, Salazar, Yeniffer Tatiana Moreno, additional, García, Juan Jesús Rubio, additional, Micó, Silvia Sevila, additional, López, Joaquín Ruiz, additional, Farré, Silvia Pérez, additional, Gomez, Maite Santamaria, additional, Petit, Nuria Mestres, additional, Titos-García, Alberto, additional, Aranda-Narváez, Jose Manuel, additional, Romacho-López, Laura, additional, Sánchez-Guillén, Luis, additional, Aranaz-Ostariz, Veronica, additional, Bosch-Ramírez, Marina, additional, Martínez-Pérez, Aleix, additional, Martínez-López, Elías, additional, Sebastián-Tomás, Juan Carlos, additional, Jimenez-Riera, Granada, additional, Jimenez-Vega, Javier, additional, Cuellar, Jose Aurelio Navas, additional, Campos-Serra, Andrea, additional, Muñoz-Campaña, Anna, additional, Gràcia-Roman, Raquel, additional, Alegre, Javier Martínez, additional, Pinto, Francisca Lima, additional, O’Sullivan, Sara Nuñez, additional, Antona, Francisco Blanco, additional, Jiménez, Beatriz Muñoz, additional, López-Sánchez, Jaime, additional, Carmona, Zahira Gómez, additional, Fernández, Rocio Torres, additional, Sierra, Isabel Blesa, additional, de León, Laura Román García, additional, Moreno, Verónica Polaino, additional, Iglesias, Eva, additional, Cumplido, Paola Lora, additional, Bravo, Altea Arango, additional, Simó, Ignacio Rey, additional, Domínguez, Carlota López, additional, Caamaño, Aloia Guerreiro, additional, Lozano, Rafael Calleja, additional, Martínez, Manuel Durán, additional, Torres, Álvaro Naranjo, additional, de Quiros, Javier Tomas Morales Bernaldo, additional, Pellino, Gianluca, additional, Cloquell, Miriam Moratal, additional, Moller, Elsa García, additional, Jalal-Eldin, Sami, additional, Abdoun, Ahmed K., additional, Hamid, Hytham K. S., additional, Lohsiriwat, Varut, additional, Mongkhonsupphawan, Aitsariya, additional, Baraket, Oussama, additional, Ayed, Karim, additional, Abbassi, Imed, additional, Ali, Ali Ben, additional, Ammar, Houssem, additional, Kchaou, Ali, additional, Tlili, Ahmed, additional, Zribi, Imen, additional, Colak, Elif, additional, Polat, Suleyman, additional, Koylu, Zehra Alan, additional, Guner, Ali, additional, Usta, Mehmet Arif, additional, Reis, Murat Emre, additional, Mantoglu, Baris, additional, Gonullu, Emre, additional, Akin, Emrah, additional, Altintoprak, Fatih, additional, Bayhan, Zulfu, additional, Firat, Necattin, additional, Isik, Arda, additional, Memis, Ufuk, additional, Bayrak, Mehmet, additional, Altıntaş, Yasemin, additional, Kara, Yasin, additional, Bozkurt, Mehmet Abdussamet, additional, Kocataş, Ali, additional, Das, Koray, additional, Seker, Ahmet, additional, Ozer, Nazmi, additional, Atici, Semra Demirli, additional, Tuncer, Korhan, additional, Kaya, Tayfun, additional, Ozkan, Zeynep, additional, Ilhan, Onur, additional, Agackiran, Ibrahim, additional, Uzunoglu, Mustafa Yener, additional, Demirbas, Eren, additional, Altinel, Yuksel, additional, Meric, Serhat, additional, Hacım, Nadir Adnan, additional, Uymaz, Derya Salim, additional, Omarov, Nail, additional, Balık, Emre, additional, Tebala, Giovanni D., additional, Khalil, Hany, additional, Rana, Mridul, additional, Khan, Mansoor, additional, Florence, Charlotte, additional, Swaminathan, Christie, additional, Leo, Cosimo Alex, additional, Liasis, Lampros, additional, Watfah, Josef, additional, Trostchansky, Ivan, additional, Delgado, Edward, additional, Pontillo, Marcelo, additional, Latifi, Rifat, additional, Coimbra, Raul, additional, Edwards, Sara, additional, Lopez, Ana, additional, Velmahos, George, additional, Dorken, Ander, additional, Gebran, Anthony, additional, Palmer, Amanda, additional, Oury, Jeffrey, additional, Bardes, James M., additional, Seng, Sirivan Suon, additional, Coffua, Lauren S., additional, Ratnasekera, Asanthi, additional, Egodage, Tanya, additional, Echeverria-Rosario, Karla, additional, Armento, Isabella, additional, Napolitano, Lena M., additional, Sangji, Naveen F., additional, Hemmila, Mark, additional, Quick, Jacob A., additional, Austin, Tyler R., additional, Hyman, Theodore S., additional, Curtiss, William, additional, McClure, Amanda, additional, Cairl, Nicholas, additional, Biffl, Walter L., additional, Truong, Hung P., additional, Schaffer, Kathryn, additional, Reames, Summer, additional, Banchini, Filippo, additional, Capelli, Patrizio, additional, Coccolini, Federico, additional, Sartelli, Massimo, additional, Bravi, Francesca, additional, Vallicelli, Carlo, additional, Agnoletti, Vanni, additional, Baiocchi, Gian Luca, additional, and Catena, Fausto, additional

Published
2024
Full Text
View/download PDF

2. Predicting disease severity in multiple sclerosis using multimodal data and machine learning

Author

Andorra, Magi, primary, Freire, Ana, additional, Zubizarreta, Irati, additional, de Rosbo, Nicole Kerlero, additional, Bos, Steffan D., additional, Rinas, Melanie, additional, Høgestøl, Einar A., additional, de Rodez Benavent, Sigrid A., additional, Berge, Tone, additional, Brune-Ingebretse, Synne, additional, Ivaldi, Federico, additional, Cellerino, Maria, additional, Pardini, Matteo, additional, Vila, Gemma, additional, Pulido-Valdeolivas, Irene, additional, Martinez-Lapiscina, Elena H., additional, Llufriu, Sara, additional, Saiz, Albert, additional, Blanco, Yolanda, additional, Martinez-Heras, Eloy, additional, Solana, Elisabeth, additional, Bäcker-Koduah, Priscilla, additional, Behrens, Janina, additional, Kuchling, Joseph, additional, Asseyer, Susanna, additional, Scheel, Michael, additional, Chien, Claudia, additional, Zimmermann, Hanna, additional, Motamedi, Seyedamirhosein, additional, Kauer-Bonin, Josef, additional, Brandt, Alex, additional, Saez-Rodriguez, Julio, additional, Alexopoulos, Leonidas G., additional, Paul, Friedemann, additional, Harbo, Hanne F., additional, Shams, Hengameh, additional, Oksenberg, Jorge, additional, Uccelli, Antonio, additional, Baeza-Yates, Ricardo, additional, and Villoslada, Pablo, additional

Published
2023
Full Text
View/download PDF

3. Is It Time for Ocrelizumab Extended Interval Dosing in Relapsing Remitting MS? Evidence from An Italian Multicenter Experience During the COVID-19 Pandemic

Author

Aurora Zanghì, Carlo Avolio, Elisabetta Signoriello, Gianmarco Abbadessa, Maria Cellerino, Diana Ferraro, Christian Messina, Stefania Barone, Graziella Callari, Elena Tsantes, Patrizia Sola, Paola Valentino, Franco Granella, Francesco Patti, Giacomo Lus, Simona Bonavita, Matilde Inglese, Emanuele D’Amico, Zanghì, Aurora, Avolio, Carlo, Signoriello, Elisabetta, Abbadessa, Gianmarco, Cellerino, Maria, Ferraro, Diana, Messina, Christian, Barone, Stefania, Callari, Graziella, Tsantes, Elena, Sola, Patrizia, Valentino, Paola, Granella, Franco, Patti, Francesco, Lus, Giacomo, Bonavita, Simona, Inglese, Matilde, and D'Amico, Emanuele

Subjects
Pharmacology, Multiple Sclerosis, Pandemic, COVID-19, Gadolinium, Extended interval dosing, Standard interval dosing, Cytidine Diphosphate, Immunologic Factor, Multiple Sclerosis, Relapsing-Remitting, Recurrence, Humans, Immunologic Factors, Pharmacology (medical), Disease activity, Ocrelizumab, Neurology (clinical), Pandemics, Sudden Infant Death, MRI, Human
Abstract

In the COVID-19 pandemic era, safety concerns have been raised regarding the risk of severe infection following administration of ocrelizumab (OCR), a B-cell-depleting therapy. We enrolled all relapsing remitting multiple sclerosis (RRMS) patients who received maintenance doses of OCR from January 2020 to June 2021. Data were extracted in December 2021. Standard interval dosing (SID) was defined as a regular maintenance interval of OCR infusion every 6 months, whereas extended interval dosing (EID) was defined as an OCR infusion delay of at least 4 weeks. Three infusions were considered in defining SID vs. EID (infusions A, B, and C). Infusion A was the last infusion before January 2020. The primary study outcome was a comparison of disease activity during the A-C interval, which was defined as either clinical (new relapses) or radiological (new lesions on T1-gadolinium or T2-weighted magnetic resonance imaging (MRI) sequences). Second, we aimed to assess confirmed disability progression (CDP). A total cohort of 278 patients (174 on SID and 104 on EID) was enrolled. Patients who received OCR on EID had a longer disease duration and a higher rate of vaccination against severe acute respiratory syndrome-coronavirus 2 (p

Published
2022
Full Text
View/download PDF

4. What happens after fingolimod discontinuation? A multicentre real-life experience

Author

Eleonora Cocco, Maria Cellerino, Carolina Gabri Nicoletti, Girolama Alessandra Marfia, Marta Ponzano, Marzia Fronza, Giacomo Boffa, Maria Elena Ricchiuto, Elisabetta Mancuso, Jessica Frau, Doriana Landi, Giuseppe Fenu, Matilde Inglese, and Alessio Signori

Subjects
Adult, medicine.medical_specialty, Multiple Sclerosis, Neurology, Discontinuation, Relapsing-Remitting, Settore MED/26, Logistic regression, 03 medical and health sciences, Multiple Sclerosis, Relapsing-Remitting, 0302 clinical medicine, Internal medicine, Humans, Medicine, 030212 general & internal medicine, Risk factor, Expanded Disability Status Scale, Fingolimod Hydrochloride, business.industry, Multiple sclerosis, Fingolimod, Reactivation, medicine.disease, Magnetic Resonance Imaging, Lymphocyte count, Cohort, Female, Neurology (clinical), business, Immunosuppressive Agents, 030217 neurology & neurosurgery, medicine.drug
Abstract

To analyse the course of multiple sclerosis (MS) after fingolimod withdrawal in a multicentre cohort. Patients who discontinued fingolimod were included. Relapses, Expanded Disability Status Scale (EDSS), and new/gadolinium-enhancing lesions on magnetic resonance imaging (MRI) were assessed during the last year on fingolimod, and in the year after discontinuation. Wilcoxon test was used to analyse the difference in EDSS and relapses between the two periods, and to compare lymphocyte counts at discontinuation and 3 months later. Demographic and clinical variables were evaluated using univariable and multivariable logistic regression analyses. Patients were 230 (females 66.1%; mean age 38 years; median EDSS 3). Fingolimod was discontinued due to inefficacy in 57%, and 87.4% started another treatment. Relapse was observed in 33% of the patients in the year after discontinuation. Severe reactivation was observed in 15%. During the first 6 months after discontinuation, new/enhancing lesions were seen in 62/116 patients. Higher age at the fingolimod discontinuation was found to be associated with a lower probability of inflammatory activity (p = 0.001) and severe reactivation (p = 0.007) during the year after discontinuation. Lower lymphocyte count was a risk factor for clinical, radiological, and severe activity (p = 0.02, p = 0.002, p = 0.01, respectively). The main reason for the discontinuation of fingolimod was inefficacy. One-third of the patients had a relapse during the year after discontinuation, 15% experienced a severe reactivation, and approximately 50% of patients with available MRI scan had new/enhancing lesions. The risk factors for disease activity after discontinuation were low lymphocyte count and younger age.

Published
2021
Full Text
View/download PDF

5. Is It Time for Ocrelizumab Extended Interval Dosing in Relapsing Remitting MS? Evidence from An Italian Multicenter Experience During the COVID-19 Pandemic

Author

Zanghì, Aurora, primary, Avolio, Carlo, additional, Signoriello, Elisabetta, additional, Abbadessa, Gianmarco, additional, Cellerino, Maria, additional, Ferraro, Diana, additional, Messina, Christian, additional, Barone, Stefania, additional, Callari, Graziella, additional, Tsantes, Elena, additional, Sola, Patrizia, additional, Valentino, Paola, additional, Granella, Franco, additional, Patti, Francesco, additional, Lus, Giacomo, additional, Bonavita, Simona, additional, Inglese, Matilde, additional, and D’Amico, Emanuele, additional

Published
2022
Full Text
View/download PDF

7. Nothobranchius annual killifishes

Author

Alessandro Cellerino, Eva Terzibasi Tozzini, Terzibasi Tozzini, Eva, and Cellerino, Alessandro

Subjects
Aging, Teleost, Ecology (disciplines), lcsh:Evolution, Zoology, Genomics, Review, Diapause, Settore BIO/09 - Fisiologia, biology.animal, Comparative genomic, lcsh:QH359-425, Genetics, Neurodegeneration, CRISPR/Cas9, Ecology, Evolution, Behavior and Systematics, Comparative genomics, biology, Extreme habitat, Life history adaptation, Vertebrate, biology.organism_classification, Nothobranchius, Habitat, Transgenesis, RNA-seq, Parallel evolution, Developmental Biology
Abstract

Annual fishes of the genus Nothobranchius inhabit ephemeral habitats in Eastern and Southeastern Africa. Their life cycle is characterized by very rapid maturation, a posthatch lifespan of a few weeks to months and embryonic diapause to survive the dry season. The speciesN. furzeriholds the record of the fastest-maturing vertebrate and of the vertebrate with the shortest captive lifespan and is emerging as model organism in biomedical research, evolutionary biology, and developmental biology. Extensive characterization of age-related phenotypes in the laboratory and of ecology, distribution, and demography in the wild are available. Species/populations from habitats differing in precipitation intensity show parallel evolution of lifespan and age-related traits that conform to the classical theories on aging. Genome sequencing and the establishment of CRISPR/Cas9 techniques made this species particularly attractive to investigate the effects genetic and non-genetic intervention on lifespan and aging-related phenotypes. At the same time, annual fishes are a very interesting subject for comparative approaches, including genomics, transcriptomics, and proteomics. TheN. furzericommunity is highly diverse and rapidly expanding and organizes a biannual meeting.

Published
2020
Full Text
View/download PDF

8. Membrane lipids and maximum lifespan in clownfish

Author

Almaida-Pagan, Pedro F., primary, Lucas-Sanchez, Alejandro, additional, Martinez-Nicolas, Antonio, additional, Terzibasi, Eva, additional, de Lama, Maria Angeles Rol, additional, Cellerino, Alessandro, additional, Mendiola, Pilar, additional, and de Costa, Jorge, additional

Published
2021
Full Text
View/download PDF

9. High HIV/STI Test Acceptance Through a Behavioral Health Encounter in Latino Immigrants with Substance Use and Mental Health Problems

Author

Nabila El-Bassel, Dianna L. Mejia, Julie H. Levison, Larmiar Fuentes, Lucía Cellerino, Margarita Alegría, Sheri Lapatin Markle, Ye Wang, Andrew Tarbox, and Lucía Albarracín García

Subjects
Adult, Male, medicine.medical_specialty, Adolescent, Social Psychology, Substance-Related Disorders, Population, Sexually Transmitted Diseases, Emigrants and Immigrants, HIV Infections, Article, law.invention, Odds, 03 medical and health sciences, 0302 clinical medicine, Randomized controlled trial, law, medicine, Humans, Mass Screening, Serologic Tests, 030212 general & internal medicine, education, Aged, Multinomial logistic regression, education.field_of_study, 030505 public health, business.industry, Mental Disorders, Public health, Public Health, Environmental and Occupational Health, virus diseases, Hispanic or Latino, Middle Aged, Patient Acceptance of Health Care, Mental health, Clinical trial, Health psychology, Mental Health, Sexual Partners, Infectious Diseases, Spain, Family medicine, Female, Lost to Follow-Up, 0305 other medical science, business, Boston
Abstract

Latino immigrants with substance use and mental health problems are at risk for undiagnosed HIV and sexually transmitted infections (STIs). Participants in a randomized control trial were recruited in Boston, USA and Madrid and Barcelona, Spain. Eligibility criteria were Latino self-identification, age 18-70, elevated substance use and mental health symptoms, and not currently in substance or mental health care. A multinomial logistic regression examined predictors of HIV/STI testing decline and lost to follow-up (LTFU) prior to testing compared with acceptance. Of 341 participants, 74% accepted testing, 4% declined, and 22% were LTFU. The odds of LTFU were higher in those with high concern for HIV and those whose main partner had done HIV testing. Age ≥ 35 years, females, higher education, and higher report of discrimination lowered the odds of LTFU. Delivery of HIV/STI testing through community agencies and outreach could overcome barriers to HIV/STI diagnosis in this population of Latinos.Clinical Trial Number: NCT02038855.

Published
2019
Full Text
View/download PDF

10. Predictors of Ocrelizumab Effectiveness in Patients with Multiple Sclerosis

Author

Cellerino, Maria, primary, Boffa, Giacomo, additional, Lapucci, Caterina, additional, Tazza, Francesco, additional, Sbragia, Elvira, additional, Mancuso, Elisabetta, additional, Bruschi, Nicolò, additional, Minguzzi, Simona, additional, Ivaldi, Federico, additional, Poirè, Ilaria, additional, Laroni, Alice, additional, Mancardi, Gianluigi, additional, Capello, Elisabetta, additional, Uccelli, Antonio, additional, Novi, Giovanni, additional, and Inglese, Matilde, additional

Published
2021
Full Text
View/download PDF

11. What happens after fingolimod discontinuation? A multicentre real-life experience

Author

Landi, Doriana, primary, Signori, Alessio, additional, Cellerino, Maria, additional, Fenu, Giuseppe, additional, Nicoletti, Carolina Gabri, additional, Ponzano, Marta, additional, Mancuso, Elisabetta, additional, Fronza, Marzia, additional, Ricchiuto, Maria Elena, additional, Boffa, Giacomo, additional, Inglese, Matilde, additional, Marfia, Girolama Alessandra, additional, Cocco, Eleonora, additional, and Frau, Jessica, additional

Published
2021
Full Text
View/download PDF

14. Limited scope for reproductive senescence in wild populations of a short-lived fish

Author

Jakub Žák, Alessandro Cellerino, Milan Vrtílek, Martin Reichard, Matej Polačik, Radim Blažek, Vrtilek, M., Zak, J., Blazek, R., Polacik, M., Cellerino, A., and Reichard, M.

Subjects
Male, 0106 biological sciences, 0301 basic medicine, Senescence, Aging, Plasticity, Longevity, Population, Captivity, Zoology, Animals, Wild, Population heterogeneity, 010603 evolutionary biology, 01 natural sciences, Nothobranchius furzeri, Cyprinodontiformes, 03 medical and health sciences, Reproductive senescence, Animals, Gene by environment interaction, Killifish, Gonads, education, Ecology, Evolution, Behavior and Systematics, Turquoise killifish, education.field_of_study, biology, Reproduction, General Medicine, biology.organism_classification, Fecundity, Reproductive ageing, 030104 developmental biology, Female
Abstract

Senescence in wild populations was long considered negligible but current evidence suggests that it is widespread in natural populations of mammals and birds, affecting the survival and reproductive output of older individuals. In contrast, little is known about reproductive senescence in species with asymptotic growth that can keep increasing their reproductive output as they grow older and larger. Using a cross-sectional study, we tested age-related decline in fecundity and relative allocation to reproduction in five wild populations of an annual killifish, Nothobranchius furzeri (Cyprinodontiformes). We did not detect any decline in absolute female egg production over their short lifespan in the wild. Relative fecundity (egg production controlled for female body mass) tended to decrease with age. This effect was driven primarily by a single population that survived 17weeks, almost twice as long as the median persistence of the other four study populations. There was no decrease in relative ovary mass while in males, relative testes mass actually increased with age. Intra-population variation in relative ovary mass increased in older females suggesting heterogeneity in individual trajectories of female reproductive allocation. Overall, we demonstrate that annual killifish do not experience significant age-related decline in reproductive functions during their very short lifespan in the wild despite the marked deterioration of gonad tissue detected in captivity.

Published
2018
Full Text
View/download PDF

15. Correction to: A miRNA catalogue and ncRNA annotation of the short-living fish Nothobranchius furzeri

Author

Aurora Savino, Mario Baumgart, Manja Marz, Emanuel Barth, Ivan Arisi, Alessandro Cellerino, Matthias Platzer, Philipp Koch, Andreas Petzold, and Marco Groth

Subjects
0106 biological sciences, Aging, RNA, Untranslated, lcsh:QH426-470, lcsh:Biotechnology, Longevity, Computational biology, 01 natural sciences, Nothobranchius furzeri, Cyprinodontiformes, 03 medical and health sciences, Annotation, lcsh:TP248.13-248.65, Gene Duplication, Genetics, Animals, Gene Library, 030304 developmental biology, 0303 health sciences, biology, miRNome, Correction, Molecular Sequence Annotation, biology.organism_classification, Non-coding RNA, ncRNA, lcsh:Genetics, MicroRNAs, Fish miRNA evolution, %22">Fish , Research Article, 010606 plant biology & botany, Biotechnology
Abstract

Background The short-lived fish Nothobranchius furzeri is the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated in age dependent control of gene expression. Results Here, we present a comprehensive catalogue of miRNAs and several other non-coding RNA classes (ncRNAs) for Nothobranchius furzeri. Analyzing multiple small RNA-Seq libraries, we show most of these identified miRNAs are expressed in at least one of seven Nothobranchius species. Additionally, duplication and clustering of N. furzeri miRNAs was analyzed and compared to the four fish species Danio rerio, Oryzias latipes, Gasterosteus aculeatus and Takifugu rubripes. A peculiar characteristic of N. furzeri, as compared to other teleosts, was a duplication of the miR-29 cluster. Conclusion The completeness of the catalogue we provide is comparable to that of the zebrafish. This catalogue represents a basis to investigate the role of miRNAs in aging and development in this species. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3951-8) contains supplementary material, which is available to authorized users.

Published
2019
Full Text
View/download PDF

16. A miRNA catalogue and ncRNA annotation of the short-living fish Nothobranchius furzeri

Author

Aurora Savino, Mario Baumgart, Emanuel Barth, Marco Groth, Manja Marz, Alessandro Cellerino, Philipp Koch, Matthias Platzer, Ivan Arisi, Andreas Petzold, Baumgart, Mario, Barth, Emanuel, Savino, Aurora, Groth, Marco, Koch, Philipp, Petzold, Andrea, Arisi, Ivan, Platzer, Matthia, Marz, Manja, and Cellerino, Alessandro

Subjects
NcRNA, 0301 basic medicine, MiRNome, lcsh:QH426-470, Takifugu rubripes, lcsh:Biotechnology, Oryzias, Danio, Settore BIO/09 - Fisiologia, Nothobranchius furzeri, 03 medical and health sciences, 0302 clinical medicine, lcsh:TP248.13-248.65, biology.animal, Genetics, Zebrafish, miRNome, Fish miRNA evolution, ncRNA, biology, Vertebrate, biology.organism_classification, Non-coding RNA, lcsh:Genetics, 030104 developmental biology, Nothobranchius, Evolutionary biology, 030217 neurology & neurosurgery, Biotechnology
Abstract

Background: The short-lived fish Nothobranchius furzeri is the shortest-lived vertebrate that can be cultured in captivity and was recently established as a model organism for aging research. Small non-coding RNAs, especially miRNAs, are implicated in age-dependent control of gene expression.Results: Here, we present a comprehensive catalogue of miRNAs and several other non-coding RNA classes (ncRNAs) for Nothobranchius furzeri. Analyzing multiple small RNA-Seq libraries, we show most of these identified miRNAs are expressed in at least one of seven Nothobranchius species. Additionally, duplication and clustering of N. furzeri miRNAs was analyzed and compared to the four fish species Danio rerio, Oryzias latipes, Gasterosteus aculeatus and Takifugu rubripes. A peculiar characteristic of N. furzeri as compared to other teleosts was a duplication of the miR-29 cluster.Conclusion: The completeness of the catalogue we provide is comparable to that of zebrafish. This catalogue represents a basis to investigate the role of miRNAs in aging and development in this species.Availability: All supplementary material can be found online at http://www.rna.uni-jena.de/en/supplements/nothobranchius-furzeri-mirnome/.

Published
2017
Full Text
View/download PDF

17. MicroRNA miR-29 controls a compensatory response to limit neuronal iron accumulation during adult life and aging

Author

Eva Terzibasi Tozzini, Valeria Arcucci, Marco Terrigno, Luca Pandolfini, Roberto Ripa, Luca Dolfi, Alessandro Cellerino, Mario Baumgart, Aurora Savino, Marco Groth, Ripa, Roberto, Dolfi, Luca, Terrigno, Marco, Pandolfini, Luca, Savino, Aurora, Arcucci, Valeria, Groth, Marco, Terzibasi Tozzini, Eva, Baumgart, Mario, and Cellerino, Alessandro

Subjects
Genetics and Molecular Biology (all), 0301 basic medicine, Aging, KEGG Pathway Analysis, Iron Homeostasis, Iron Accumulation, Multisite Gateway, Dietary Iron Supplementation, Physiology, Messenger, Plant Science, medicine.disease_cause, Settore BIO/09 - Fisiologia, 0302 clinical medicine, Models, Structural Biology, Gene expression, Homeostasis, Aging brain, Animals, Base Sequence, Brain, Iron, Iron Overload, Iron Regulatory Protein 2, Killifishes, MicroRNAs, Models, Biological, Neurons, RNA, Messenger, Up-Regulation, Zebrafish, Agricultural and Biological Sciences(all), Phenotype, Cell biology, General Agricultural and Biological Sciences, Intracellular, Research Article, Biotechnology, Transferrin receptor, Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Downregulation and upregulation, microRNA, medicine, Ecology, Evolution, Behavior and Systematics, Biochemistry, Genetics and Molecular Biology(all), Cell Biology, Biological, 030104 developmental biology, RNA, 030217 neurology & neurosurgery, Oxidative stress, Neuroscience, Developmental Biology
Abstract

Background A widespread modulation of gene expression occurs in the aging brain, but little is known as to the upstream drivers of these changes. MicroRNAs emerged as fine regulators of gene expression in many biological contexts and they are modulated by age. MicroRNAs may therefore be part of the upstream drivers of the global gene expression modulation correlated with aging and aging-related phenotypes. Results Here, we show that microRNA-29 (miR-29) is induced during aging in short-lived turquoise killifish brain and genetic antagonism of its function induces a gene-expression signature typical of aging. Mechanicistically, we identified Ireb2 (a master gene for intracellular iron delivery that encodes for IRP2 protein), as a novel miR-29 target. MiR-29 is induced by iron loading and, in turn, it reduces IRP2 expression in vivo, therefore limiting intracellular iron delivery in neurons. Genetically modified fish with neuro-specific miR-29 deficiency exhibit increased levels of IRP2 and transferrin receptor, increased iron content, and oxidative stress. Conclusions Our results demonstrate that age-dependent miR-29 upregulation is an adaptive mechanism that counteracts the expression of some aging-related phenotypes and its anti-aging activity is primarily exerted by regulating intracellular iron homeostasis limiting excessive iron-exposure in neurons. Electronic supplementary material The online version of this article (doi:10.1186/s12915-017-0354-x) contains supplementary material, which is available to authorized users.

Published
2017
Full Text
View/download PDF

18. Comparison of captive lifespan, age-associated liver neoplasias and age-dependent gene expression between two annual fish species: Nothobranchius furzeri and Nothobranchius korthause

Author

Eva Terzibasi Tozzini, Giacomo Rossi, Alessandro Cellerino, Mario Baumgart, Emiliano Di Cicco, Baumgart, Mario, Di Cicco, E, Rossi, G, Cellerino, Alessandro, and Terzibasi, Eva

Subjects
Male, Aging, media_common.quotation_subject, Longevity, Fish species, Gene Expression, Zoology, Age dependent, Biology, Africa, Southern, Collagen Type I, Nothobranchius furzeri, Cyprinodontiformes, Species Specificity, Gene expression, Animals, RNA, Messenger, Cyclin B1, Skin, media_common, Ecology, Incidence, Liver Neoplasms, Brain, biology.organism_classification, Nothobranchius, Ageing, Female, Seasons, Geriatrics and Gerontology, Gerontology, Developmental biology, Biomarkers
Abstract

Nothobranchius is a genus of annual fish broadly distributed in South-Eastern Africa and found into temporary ponds generated during the rain seasons and their lifespan is limited by the duration of their habitats. Here we compared two Nothobranchius species from radically different environments: N. furzeri and N. korthausae. We found a large difference in life expectancy (29- against 71-weeks of median life span, 40- against 80-weeks of maximum lifespan, respectively), which correlates with a diverse timing in the onset of several age dependent processes: our data show that N. korthause longer lifespan is associated to retarded onset of age-dependent liver-neoplasia and slower down-regulation of collagen 1 alpha 2 (COL1A2) expression in the skin. On the other hand, the expression of cyclin B1 (CCNB1) in the brain was strongly age-regulated, but with similar profiles in N. furzeri and N. korthausae. In conclusion, our data suggest that the different ageing rate of two species of the same genus could be used as novel tool to investigate and better understand the genetic bases of some general mechanism leading to the complex ageing process, providing a strategy to unravel some of the genetic mechanisms regulating longevity and age-associate pathologies including neoplasias.

Published
2014
Full Text
View/download PDF

21. Publisher Correction: Transcriptomic alterations during ageing reflect the shift from cancer to degenerative diseases in the elderly

Author

Irizar, Peer Aramillo, primary, Schäuble, Sascha, additional, Esser, Daniela, additional, Groth, Marco, additional, Frahm, Christiane, additional, Priebe, Steffen, additional, Baumgart, Mario, additional, Hartmann, Nils, additional, Marthandan, Shiva, additional, Menzel, Uwe, additional, Müller, Jule, additional, Schmidt, Silvio, additional, Ast, Volker, additional, Caliebe, Amke, additional, König, Rainer, additional, Krawczak, Michael, additional, Ristow, Michael, additional, Schuster, Stefan, additional, Cellerino, Alessandro, additional, Diekmann, Stephan, additional, Englert, Christoph, additional, Hemmerich, Peter, additional, Sühnel, Jürgen, additional, Guthke, Reinhard, additional, Witte, Otto W., additional, Platzer, Matthias, additional, Ruppin, Eytan, additional, and Kaleta, Christoph, additional

Published
2019
Full Text
View/download PDF

24. Sub-cellular localization analysis of MSH6 missense mutations does not reveal an overt MSH6 nuclear transport impairment

Author

Francesca Bianchi, L. Belvederesi, Riccardo Cellerino, Cristian Loretelli, R. Bracci, Stefano Cascinu, L., Belvederesi, F., Bianchi, C., Loretelli, R., Bracci, Cascinu, Stefano, and R., Cellerino

Subjects
congenital, hereditary, and neonatal diseases and abnormalities, Cancer Research, Recombinant Fusion Proteins, Green Fluorescent Proteins, Active Transport, Cell Nucleus, Mutation, Missense, Biology, medicine.disease_cause, DNA Mismatch Repair, Germline mutation, Genetics, medicine, Humans, Missense mutation, Genetics (clinical), Cellular localization, Mutation, nutritional and metabolic diseases, Prognosis, medicine.disease, Colorectal Neoplasms, Hereditary Nonpolyposis, digestive system diseases, Lynch syndrome, DNA-Binding Proteins, MSH6, Oncology, Mutant Proteins, DNA mismatch repair, Nuclear transport, Subcellular Fractions
Abstract

Nearly one-third of the identified MSH6 germline mutations deal with single amino acid substitutions. For an effective genetic counselling it is necessary to clearly elucidate by functional tools the specific sub-processes underlying the mismatch repair (MMR) misfunctioning in MSH6 non-truncating mutants. Since the MMR repair pathway occurs in the nucleus, we suppose the impairment of MutSα nuclear trafficking to be a possible Lynch syndrome susceptibility causative mechanism. In the present study the MMR status of the tumour, the main clinical features of mutation carriers and population data associated to the MSH6 missense variants, were complemented with computational data about tolerability predictions and amino acid substitution conservation. The selected panel of ten potentially pathogenic MSH6 mutations was analyzed in a homologous expression system for possible deleterious effects on nucleo-cytoplasmic shuttling through the assessment of the sub-cellular localization of the corresponding mutated proteins. Localization analysis results do not reveal an apparent role of MSH6 missense mutations in nuclear import impairment and provide the first hint to exclude the MSH6 nuclear translocation sub-process as a possible causative mechanisms of MMR misfunctioning.

Published
2012
Full Text
View/download PDF

27. Transcriptomic alterations during ageing reflect the shift from cancer to degenerative diseases in the elderly

Author

Aramillo Irizar, Peer, primary, Schäuble, Sascha, additional, Esser, Daniela, additional, Groth, Marco, additional, Frahm, Christiane, additional, Priebe, Steffen, additional, Baumgart, Mario, additional, Hartmann, Nils, additional, Marthandan, Shiva, additional, Menzel, Uwe, additional, Müller, Jule, additional, Schmidt, Silvio, additional, Ast, Volker, additional, Caliebe, Amke, additional, König, Rainer, additional, Krawczak, Michael, additional, Ristow, Michael, additional, Schuster, Stefan, additional, Cellerino, Alessandro, additional, Diekmann, Stephan, additional, Englert, Christoph, additional, Hemmerich, Peter, additional, Sühnel, Jürgen, additional, Guthke, Reinhard, additional, Witte, Otto W., additional, Platzer, Matthias, additional, Ruppin, Eytan, additional, and Kaleta, Christoph, additional

Published
2018
Full Text
View/download PDF

28. Age-dependent increase of oxidative stress regulates microRNA-29 family preserving cardiac health

Author

Heid, Johanna, primary, Cencioni, Chiara, additional, Ripa, Roberto, additional, Baumgart, Mario, additional, Atlante, Sandra, additional, Milano, Giuseppina, additional, Scopece, Alessandro, additional, Kuenne, Carsten, additional, Guenther, Stefan, additional, Azzimato, Valerio, additional, Farsetti, Antonella, additional, Rossi, Giacomo, additional, Braun, Thomas, additional, Pompilio, Giulio, additional, Martelli, Fabio, additional, Zeiher, Andreas M., additional, Cellerino, Alessandro, additional, Gaetano, Carlo, additional, and Spallotta, Francesco, additional

Published
2017
Full Text
View/download PDF

31. A missense germline mutation in exon 7 of the MSH2 gene in a HNPCC family from center-Italy

Author

Emilio Porfiri, R. Bracci, Eva Galizia, Francesca Bianchi, Italo Bearzi, R. Catalani, Cristian Loretelli, L. Belvederesi, Chiara Turchi, Alessandra Viel, and Riccardo Cellerino

Subjects
Adult, Male, congenital, hereditary, and neonatal diseases and abnormalities, Cancer Research, DNA Mutational Analysis, Mutation, Missense, Adenocarcinoma, Biology, Arginine, MLH1, Germline, Neoplasms, Multiple Primary, Germline mutation, Serine, Genetics, medicine, Humans, Missense mutation, Multiplex ligation-dependent probe amplification, Germ-Line Mutation, Genetics (clinical), Aged, Aged, 80 and over, Microsatellite instability, Exons, Middle Aged, medicine.disease, Colorectal Neoplasms, Hereditary Nonpolyposis, Kidney Neoplasms, digestive system diseases, Pedigree, MutS Homolog 2 Protein, Amino Acid Substitution, Italy, Molecular Diagnostic Techniques, Oncology, MSH2, Mutation (genetic algorithm), Cancer research, Female, Microsatellite Instability, Oligonucleotide Probes
Abstract

Introduction Hereditary Non-Polyposis Colorectal Cancer (HNPCC) is an autosomal dominant inherited disease predisposing to the development of colorectal cancers and several other malignancies (endometrium, ovaries, stomach, small bowel, hepatobiliary and urinary tract). HNPCC is caused by germline mutations in any of the MisMatch Repair (MMR) genes. Mutations in MLH1 and MSH2 account for almost 90% of all identified ones. About 15% of mutations identified in MSH2 are missense ones. Patients and methods We studied one family, fulfilling Amsterdam II criteria, referred to our Center for genetic counselling. The proband, and some of her relatives, have been investigated for microsatellite instability (MSI), immunohistochemical MMR protein staining and by direct sequencing and Multiplex Ligation-dependent Probe Amplification (MLPA). Results All patients carried the same novel MSH2 germline missense mutation (R359S) in exon 7, which determines the substitution of an Arginine, which is a basic amino acid, with a polar Serine residue (R359S). The mutation was associated with lack of expression of MSH2 protein and high microsatellite instability in tumour tissues. The same mutation has been detected in one healthy relative. Conclusions The mutation here reported shows a high correlation with phenotype. The mutation is located in an evolutionary conserved domain. Taken together, our findings suggest evidence that the amino acid substitution can be interpreted as pathogenetic.

Published
2006
Full Text
View/download PDF

32. Assessing the pathogenicity of MLH1 missense mutations in patients with suspected hereditary nonpolyposis colorectal cancer: correlation with clinical, genetic and functional features

Author

S. Rosati, Riccardo Cellerino, Italo Bearzi, Eva Galizia, Daniela Gagliardini, Cristian Loretelli, L. Belvederesi, Alessandra Viel, Francesca Bianchi, Emilio Porfiri, and R. Bracci

Subjects
Adult, Male, congenital, hereditary, and neonatal diseases and abnormalities, DNA repair, Mutation, Missense, Biology, Transfection, medicine.disease_cause, MLH1, Cell Line, Cell Line, Tumor, Genetics, medicine, PMS2, Humans, Missense mutation, Family history, neoplasms, Genetics (clinical), Adaptor Proteins, Signal Transducing, Aged, Mismatch Repair Endonuclease PMS2, Adenosine Triphosphatases, Mutation, Nuclear Proteins, nutritional and metabolic diseases, Microsatellite instability, Sequence Analysis, DNA, Middle Aged, medicine.disease, Colorectal Neoplasms, Hereditary Nonpolyposis, digestive system diseases, DNA-Binding Proteins, DNA Repair Enzymes, MutS Homolog 2 Protein, MSH2, Case-Control Studies, Mutagenesis, Site-Directed, Female, Carrier Proteins, MutL Protein Homolog 1, Microsatellite Repeats, Protein Binding
Abstract

Assessing the pathogenicity of missense mutations of MLH1 and MSH2 is critical to counsel patients with suspected hereditary nonpolyposis colorectal cancer (HNPCC). Approximately 32% of all MLH1 mutations and 18% of MSH2 mutations are missense variants which often have an uncertain genetic significance. To assess the pathogenicity of four MLH1 missense mutations which were found in five patients with suspected HNPCC, P648S (CCC --> TCC), L559R (CTG --> CGG), K618A (AAG --> GCG), Y646C (TAT --> TGT), we studied their ability to disrupt MLH1 protein function and their relationship with all those clinical, genetic and pathological features which are typical of this syndrome. Our results indicated that the P648S and L559R mutations were probably pathogenic because they disrupted MLH1 protein interaction with its partner PMS2 in vitro and abolished MLH1 expression in HCT116 cells. In addition these variants were associated with features often found in HNPCC patients: in particular high microsatellite instability, occurrence of high grade tumours and, in one case, strong family history. The pathogenicity of the K618A and Y646C mutations was questionable as their correlation with features typical of HNPCC was low and the outcome of the functional analysis was ambiguous. These observations suggested that a clinically usable assessment of the pathogenicity of MLH missense variants can be achieved through the analysis of multiple mutation characteristics among which loss of protein function, occurrence of microsatellite instability and family history seemed to have a predominant role.

Published
2006
Full Text
View/download PDF

33. Pain and its treatment in hospitalized patients with metastatic cancer

Author

Roberta Camisa, Stefania Munaó, Domenico Gattuso, Roberto Labianca, Renata Todeschini, Giordano D. Beretta, Giampietro Gasparini, Stefano Cascinu, Paolo Giordani, Sandro Barni, Marzia Mare, Riccardo Cellerino, Romina Agostinelli, Franca Pulita, Giuseppina Catalano, and Laura Iacorossi

Subjects
Adult, Male, medicine.medical_specialty, Hospitalized patients, Pain medicine, Pain, Nursing care, Pain control, Neoplasms, Surveys and Questionnaires, Internal medicine, medicine, Humans, Pain Management, Prospective Studies, Prospective cohort study, Aged, Pain Measurement, Inpatients, business.industry, Cancer, Middle Aged, Pain management, medicine.disease, Italy, Oncology, Multicenter study, Physical therapy, Female, business
Abstract

The aim of this prospective study was to assess the quality of pain management hospitalized cancer patients.In a quantitative and qualitative evaluation from six oncology centers in Italy, all consecutive cancer patients complaining of pain and hospitalized during the same 2 weeks were requested to fill in a McGill pain questionnaire (MPQ), a present pain intensity scale (PPI), and a hospital anxiety and depression acale (HADS), and to answer a questionnaire focused (QF) on the quality of medical and nursing care. The healthcare provider's antalgic prescriptions were assessed by an index of pain management (IPM).Of 120 patients with pain admitted to oncology divisions (65 men and 52 women; mean age 57 years, range 21-79 years), 117 completed the questionnaires. The quantitative evaluation (PPI) showed a significant pain reduction between admission and discharge pain levels-from 2.65 to 1.50 ( p0.001). While a significant reduction of anxiety (HADS) was also found-from 10.24 to 9.11 ( p0.001)-depression did not improve (9.83 and 9.72). The most relevant information from qualitative evaluation (QF) was: in 37.6% of patients, pain level was higher overnight; 47% waited for spontaneous decrease of pain intensity before asking for nurse or physician intervention; 69% asked for nurse help when pain level was really high. The health care response to patients' pain was not completely satisfactory, since analgesic prescription was adequate in 56.52% but inadequate in 43.47%.Pain control in hospitalized cancer patients is not completely satisfactory. The physician's attitude is to underestimate and undertreat pain, while nurses are not adequately trained for timely intervention despite published guidelines for pain management. The findings of this study support the concern of inadequate knowledge and inappropriate attitudes regarding pain management, even in cancer patients hospitalized in medical oncology divisions.

Published
2003
Full Text
View/download PDF

34. The mechanisms of blood vessel closure in humans by the application of Ultrasonic energy

Author

P. Cellerino, Diego Foschi, Emilio Trabucchi, Fabio Corsi, E. Morandi, T Taidelli, and Andrea Rizzi

Subjects
Ultrasonic Therapy, Lumen (anatomy), Hemorrhoids, Collagen fibril, Extracellular matrix, Necrosis, Electrocoagulation, Humans, Medicine, Ultrasonography, business.industry, Stomach, Cystic Duct, Anatomy, Endothelial stem cell, Microscopy, Electron, medicine.anatomical_structure, Coagulative necrosis, Circulatory system, Microscopy, Electron, Scanning, cardiovascular system, Surgery, Ultrasonic sensor, sense organs, business, Omentum, Vascular Surgical Procedures, Blood vessel
Abstract

The use of the ultrasonically activated scalpel (UAS) for vessel closure has attained widespread acceptance in many surgical fields. The aim of our study was to investigate the electron microscopic changes to the blood vessels after the application of UAS. We collected 10 arterial and 10 venous segments from vessels that had previously been closed by UAS during abdominal operations. The samples were then prepared for ultramicroscopic analysis Pathological changes in the lumen and the three wall layers of the blood vessel were examined under scanning and transmission electron microscopy. All of the vessel segments showed similar changes: the presence of a blood clot, endothelial cell condensation, coagulative necrosis of the wall, and charring of the vessel at its tip. The edge of the cut vessel were closed by the coagulation bond, which was tied up by collagen fibrils escaped from denaturation. When ultrasonic energy is applied to tissues, it changes their structure so as to make a new extracellular matrix.

Published
2002
Full Text
View/download PDF

35. Symptomatic, stage IV, non-small-cell lung cancer (NSCLC): response, toxicity, performance status change and symptom relief in patients treated with cisplatin, vinblastine and mitomycin-C

Author

Francesco Graziano, Pierpaolo Isidori, Diego Tummarello, and Riccardo Cellerino

Subjects
Adult, Male, Cancer Research, medicine.medical_specialty, Lung Neoplasms, Palliative care, Mitomycin, medicine.medical_treatment, Vinblastine, Toxicology, Gastroenterology, Stable Disease, Carcinoma, Non-Small-Cell Lung, Internal medicine, Antineoplastic Combined Chemotherapy Protocols, medicine, Humans, Pharmacology (medical), Karnofsky Performance Status, Lung cancer, Aged, Neoplasm Staging, Pharmacology, Chemotherapy, Performance status, business.industry, Palliative Care, Remission Induction, Respiratory disease, Middle Aged, Prognosis, medicine.disease, Surgery, Treatment Outcome, Oncology, Female, Cisplatin, business, Progressive disease, medicine.drug
Abstract

In a series of 46 symptomatic patients with metastatic, stage IV, non-small-cell lung cancer (NSCLC), we used a three-drug combination with cisplatin (120 mg/m2), vinblastine (6 mg/m2) and mitomycin-C (6 mg/m2) (PVM), repeated every 3 weeks. After two courses, we observed that none of the patients had achieved a complete response; 33% (15/46) had partial response (95% confidence limits: 19.2-46.8); 39% (18/46), stable disease and 28% (13/46), progressive disease. Median response duration was 14.0 weeks (range, 4-36.7), median time to progression 22.4 weeks (range, 7-44.4), and median survival time 26.4 weeks (range, 1-103). WHO grade III-IV myelotoxicity occurred in 15.2% of the courses administered, affecting 39.5% of patients, and severe nephrotoxicity was observed in 9.3% of patients. No toxic death occurred. The post-treatment KPS score increased in 7 patients with partial response (47%), 4 with stable disease (22%) and 1 with progressive disease (8%), while it decreased in 3 patients with partial response (20%), 3 with stable disease (17%) and 10 with progressive disease (77%). In all, KPS increased in 12/46 cases (26%). However, no statistically significant difference was observed when the KPS score before and after treatment was compared in the total group of patients or when it was compared in the total group of patients or when it was compared in responders and in non-responders. After chemotherapy, there was complete disappearance of at least one symptom in 27.1% of cases and improvement in 27.1%. Overall, major symptom control occurred in 54.3% of cases, with a median palliation time lasting 10 weeks (range, 4-32). Patients with partial remission and stable disease achieved symptomatic palliation in 90% and 55.5% of cases, respectively. When we compared the palliation rate between responders and non-responders, a significant difference was noted (Chi-square test: P0.05). Although our schedule did not produce a higher objective response rate and the KPS score was not significantly improved, the symptom palliation appeared worthwhile considering the highly unfavourable prognosis of the patients investigated.

Published
1995
Full Text
View/download PDF

36. MicroRNA 19a replacement partially rescues fin and cardiac defects in zebrafish model of Holt Oram syndrome

Author

Chiavacci, Elena, primary, D’Aurizio, Romina, additional, Guzzolino, Elena, additional, Russo, Francesco, additional, Baumgart, Mario, additional, Groth, Marco, additional, Mariani, Laura, additional, D’Onofrio, Mara, additional, Arisi, Ivan, additional, Pellegrini, Marco, additional, Cellerino, Alessandro, additional, Cremisi, Federico, additional, and Pitto, Letizia, additional

Published
2015
Full Text
View/download PDF

37. Aminoglutethimide in advanced breast cancer: plasma levels and clinical results after low and high doses

Author

Carlo Maurizio Camaggi, Andrea Martoni, Elena Strocchi, S. Miseria, G. Robustelli Della Cuna, M. Balli, Ettore Bichisao, G. Bonciarelli, G. Ambroso, P. Malacarne, M. Indelli, Franco Pannuti, and R. Cellerino

Subjects
Adult, Cancer Research, medicine.medical_specialty, Metabolite, medicine.medical_treatment, Biological Availability, Breast Neoplasms, Toxicology, Drug Administration Schedule, chemistry.chemical_compound, Pharmacokinetics, Oral administration, Internal medicine, Cluster Analysis, Humans, Medicine, Pharmacology (medical), Chromatography, High Pressure Liquid, Aged, Hydrocortisone, Pharmacology, Analysis of Variance, Chemotherapy, Dose-Response Relationship, Drug, business.industry, Remission Induction, Discriminant Analysis, Middle Aged, medicine.disease, Aminoglutethimide, Endocrinology, Oncology, chemistry, Drug Evaluation, Regression Analysis, Female, business, Progressive disease, medicine.drug, Hormone
Abstract

Drug plasma levels, metabolism data and clinical results were evaluated after the daily administration of either 500 or 1,000 mg aminoglutethimide (AG, Orimeten, Ciba-Geigy) plus hydrocortisone acetate (20 mg b. i. d.). A total of 34 patients with advanced breast cancer entered the study: 17 were given 1,000 mg/day and 17 received 500 mg/day for at least 3 months. A novel HPLC method was developed to determine the levels of AG and its known metabolites [N-acetyl-AG (NAG), formyl-AG, nitroglutethimide, hydroxy-AG] in the biological samples. AG plasma concentration was significantly higher during the 1,000-mg/day regimen. NAG was the only metabolite observed in plasma, always occurring at concentrations lower than those of the parent drug. The ratios between NAG and AG levels distinguish two statistically different groups of patients. Irrespective of the dose, a partial response was observed in 44% of the patients; no change in 32% of cases; and progressive disease had an incidence of 24%. The probability of response was not dependent on the drug AUC or on the NAG/AG ratio and did not significantly depend on previous hormone treatment. Neither the plasmatic level of the AG or metabolite concentrations nor the NAG/AG ratio seemed to affect the incidence of side effects.

Published
1991
Full Text
View/download PDF

38. Late rejection of the mesh after laparoscopic hernia repair

Author

A Trabucchi, P. Cellerino, Emilio Trabucchi, Fabio Corsi, and Diego Foschi

Subjects
Male, medicine.medical_specialty, Time Factors, medicine.medical_treatment, Fistula, Iliac fossa, Hernia, Inguinal, Infections, medicine, Humans, Hernia, Laparoscopy, medicine.diagnostic_test, Groin, business.industry, Foreign-Body Reaction, Middle Aged, Surgical Mesh, Hernia repair, medicine.disease, Endoscopy, Surgery, medicine.anatomical_structure, Surgical mesh, Radiology, business
Abstract

We report the first case of late rejection of a mesh after laparoscopic hernia repair. It occurred in a 48-year-old man who had had a laparoscopic hernia repair by transabdominal preperitoneal approach 3 years earlier. The most characteristic finding was the slow development of a firm mass in the right groin, without pain or fistula. At admission 3 months later, US and CT scans demonstrated a necrotic mass extending into both iliac fossa. The mass was approached through a midline incision. Pus was taken for microscopic examination (negative), and the mesh was removed, along with several staples. Ultramicroscopic examination of the mesh showed breakdown of the fibers, collagen reduction, and no chronic inflammatory cells. No infectious cause of inflammation was identified.

Published
1998
Full Text
View/download PDF

48. Potential negative impacts and low effectiveness in the use of African annual killifish in the biocontrol of aquatic mosquito larvae in temporary water bodies

Author

Reichard, Martin, primary, Watters, Brian R, additional, Wildekamp, Rudolf H, additional, Sonnenberg, Rainer, additional, Nagy, Béla, additional, Polačik, Matej, additional, Valdesalici, Stefano, additional, Cellerino, Alessandro, additional, Cooper, Barry J, additional, Hengstler, Holger, additional, Rosenstock, John, additional, and Sainthouse, Ian, additional

Published
2010
Full Text
View/download PDF

49. High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research

Author

Reichwald, Kathrin, primary, Lauber, Chris, additional, Nanda, Indrajit, additional, Kirschner, Jeanette, additional, Hartmann, Nils, additional, Schories, Susanne, additional, Gausmann, Ulrike, additional, Taudien, Stefan, additional, Schilhabel, Markus B, additional, Szafranski, Karol, additional, Glöckner, Gernot, additional, Schmid, Michael, additional, Cellerino, Alessandro, additional, Schartl, Manfred, additional, Englert, Christoph, additional, and Platzer, Matthias, additional

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results