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Your search keyword '"CHEN, Hao-Wei"' showing total 11 results
Start Over Author "CHEN, Hao-Wei" Publisher springer science and business media llc
11 results on '"CHEN, Hao-Wei"'

4. Retraction Note: Three SAUR proteins SAUR76, SAUR77 and SAUR78 promote plant growth in Arabidopsis

Author

Li, Zhi-Gang, Chen, Hao-Wei, Li, Qing-Tian, Tao, Jian-Jun, Bian, Xiao-Hua, Ma, Biao, Zhang, Wan-Ke, Chen, Shou-Yi, and Zhang, Jin-Song

Subjects
Proteasome Endopeptidase Complex, Genotype, Science, Molecular Sequence Data, Arabidopsis, Receptors, Cell Surface, Article, Plant Growth Regulators, Gene Expression Regulation, Plant, Immunoprecipitation, Protein Isoforms, Amino Acid Sequence, Multidisciplinary, Indoleacetic Acids, Arabidopsis Proteins, Protein Stability, Genetic Complementation Test, Gene Expression Regulation, Developmental, Ethylenes, Retraction Note, Phenotype, Proteolysis, Medicine, Cotyledon, Sequence Alignment, Protein Binding, Signal Transduction
Abstract

Ethylene perceived by a family of five receptors regulates many developmental processes in Arabidopsis. Here we conducted the yeast two-hybrid assay to screen for additional unidentified proteins that interact with subfamily II ethylene receptor ETR2. Three SAUR proteins, named SAUR76, 77 and 78, were identified to associate with both ETR2 and EIN4 in different assays. Interaction of SAUR76 and SAUR78 with ETR2 was further verified by co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) assays. Expressions of SAUR76-78 are induced by auxin and ethylene treatments. Compared with wild type, SAUR-overexpressing plants exhibit reduced ethylene sensitivity, while SAUR-RNAi lines exhibit enhanced ethylene sensitivity. Overexpressing the three SAURs partially complements the phenotype of subfamily II ethylene receptor loss-of-function double mutant etr2-3ein4-4, which has increased ethylene response and small cotyledon and rosette. saur76 mutation partially suppresses the reduced ethylene sensitivity of etr2-2. SAUR76/78 proteins are regulated by 26S proteasome system and larger tag increases their protein stability. These findings suggest that SAUR76-78 may affect ethylene receptor signaling and promote plant growth in Arabidopsis.

Published
2022

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