Search

α-Galactosidase had applied in food and feed industries for hydrolyzing raffinose series oligosaccharides (RO) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. The objective of the current work was to purify the α-galactosidase of Aspergillus foetidus ZU-G1 and compared the biochemical and hydrolytic properties of three major α-galactosidase forms (α-gal I, α-gal II and α-gal III). The molecular mass of the purified enzyme as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was 106.3, 49.7 and 109.9 kDa, respectively. Its optimum reaction temperature was 60 °C and stable below 50 °C. The optimum pH of α-gal I and α-gal III was 5.0 and α-gal II was 4.0. Under 28 °C conditions for 24 h, α-gal I was stable at pH 4.0, α-gal II was stable at pH 6.0, and α-gal III was pH 5.0. α-Galactosidase was completely inhibited by Ag+. CuSO4·5H2O and SDS were powerful inhibitors of α-gal I and α-gal III but had little effect to α-gal II. EDTA did not strongly affect α-gal I and α-gal III, while strongly affect α-gal II. CaCl2·2H2O, MgSO4·7H2O and MnSO4·7H2O were activation for α-gal I, α-gal II and α-gal III. No significant inhibition of enzymes activity was observed in the presence of raffinose, lactose as well as other sugars tested. Synthetic substrate p-nitrophenyl-α-d-galactopyranoside was not preferentially hydrolyzed than natural substrates, such as melibiose, stachyose and raffinose. Under 40 and 50 °C incubation for 1–5 h, the stachyose of soybean milk was degraded by α-gal I, α-gal II and α-gal III and strongly hydrolyzed by α-gal II, and the raffinose of soybean milk was completely hydrolyzed by α-gal II and weakly hydrolyzed by α-gal I and α-gal III. The distinct hydrolytic and biochemical properties of α-gal I, α-gal II and α-gal III further signify the α-galactosidase of A. foetidus ZU-G1 was propitious to soybean milk and related food industry.

In this paper, we present a geometric exploitation of the d’Alembert–Lagrange equation (or alternatively, Lagrange form of the d’Alembert’s principle) on a Riemannian manifold. We develop the d’Alembert–Lagrange equation in a geometric form, as well as an explicit analytic form with respect to an arbitrary frame in a coordinate neighborhood on the configuration manifold. We provide a procedure to determine the governing dynamic equations of motion. Examples are given to illustrate the new formulation of dynamic equations and their relations to alternative ones. The objective is to provide a generalized perspective of governing equations of motion and its suitability for studying complex dynamic systems subject to nonholonomic constraints.

This paper presents a new and simplified set of explicit equations of motion for constrained mechanical systems. The equations are applicable with both holonomic and nonholonomic systems and the constraints may, or may not, be ideal. It is shown that this set of equations is equivalent to governing equations developed earlier by others. The connection of these equations with Kane's equations is discussed. It is shown that the developed equations are directly applicable with controlled systems where the controlling forces and moments may be subject to constraints. Finally, a procedure is presented for determining which control force systems are equivalent. Examples are presented to demonstrate the advantages, features, and range of application of the equations.

The absolute visual magnitudes of 457 Be stars are determined from Hipparcos parallaxes, subsequently the mean absolute visual magnitudes of Be stars for luminosity classes III, IV and V are obtained. The new Mv calibration is compared with existing calibrations. It is found that Be stars are generally brighter than B stars of the corresponding spectral types, and that there is no direct correlation between absolute magnitudes and the stellar rotational velocity, these results are in agreement with some earlier results. A new and interesting result is that there is no direct correlation between near infrared excess and absolute visual magnitudes for Be stars. Moreover, possible biases, such as the Malmquist bias and the Lutz-Kelker bias, are also discussed.

The crystallization dynamics of Nylon 66/Nylon 6 blends, the crystalline/crystalline polymer blends, was analyzed by DSC under isothermal conditions. The crystal growth rate (G) and the nucleation rate (N) depended on both the degree of supercooling (ΔT) and the blend mass fraction (φ). The ΔT /Tm0 values obtained at the fixed G, which corresponded to the chemical potential difference of molecules between liquid and crystal states, and the surface free energy parameters evaluated from G and N depended on φ for blends. The results suggested that Nylon 66/Nylon 6 blends with φN66≥0.80 or φN66≤0.15 are miscible.

This paper investigates the title problem from the perspective ofdetermining which forcesystems, although different, will have the same dynamic effectwhen applied to a mechanical system.A procedure is presented for determining how differences incontrol force/moment input are nullifiedby constraints on the system. This in turn provides a basis foroptimal control. The theoreticaldevelopment is illustrated by a series of examples with a model ofa redundant manipulator – a constrained/controlled triple pendulum.

This paper presents a procedure for studying dynamics of multibodysystems subjected to impulsive constraints which may be either holonomicor nonholonomic. The procedure automatically incorporates the effects ofimpulsive constraints through its analysis. The governing equationsthemselves are developed from Kane's equations, using partial velocityvectors and partial angular velocity vectors. Explicit expressions forthe coefficients and terms of the governing equations are presented. Twosubcases are studied: (1) the constraints are instantaneously applied andcontinue to act; and (2) the constraints are instantaneously applied andlifted immediately after completion of impact. The internal impulses ateach joint and constraint impulses associated with the impulsiveconstraints are calculated. The procedure is checked with two examples,whose solutions are established.

A 3.6-kb plasmid, designated pND324, was isolated from Lactococcus lactis subsp. lactis LL57-1. Sequence analysis revealed the presence of three open reading frames, rep324, orfX1 and orfX2, which are flanked by two non-coding regions, ori324 and cisE. The minimal replication region of pND324 consists of ori324 and rep324, which is closely related to the lactococcal theta-type replicons of the pWV02/pCI305 family. pND324 was stable at both 30 degrees C and 37 degrees C, whereas derivatives that lack cisE were highly unstable at 37 degrees C, indicating that cisE is essential for thermostability. Sequences that are similar to orfX1 are commonly present in the lactococcal theta-type plasmids. The orfX2 product is homologous to TrfA, a 43-kDa protein of the E. coli theta-type plasmid RK2 required for replication and maintenance. Plasmid deletion and stability analyses showed that orfX2 is involved in the thermostability of pND324. Based on the minimal replication region of pND324, an integrative cloning vector, designated pND421, was constructed. In L. lactis LM0230, cells that carried pND421 integrated into its host chromosomal DNA could be recovered readily following incubation at 37 degrees C for 40 generations. The integrated plasmid was totally stable for at least 100 generations without selection at 30 degrees C.

In 40 patients with TNM stage T1 ovarian clear cell adenocarcinoma, we used nuclear morphometry to study the relations among morphometric variables, clinical prognostic factors and outcome. The presence of one or more giant nuclear cells was positively associated with death (OR = 10.6, P = 0.02) and tended to be associated with disease recurrence (OR = 5.1, P = 0.07). Nuclear irregularity (expressed in terms of the nuclear roundness factor) was positively associated with both death (OR = 8.6, P = 0.02) and disease recurrence (OR = 8.2, P = 0.02). A combination of giant nuclear cell presence or nuclear irregularity proved to be a useful prognostic indicator, with a sensitivity and specificity of 83% and 71% in the prediction of death, and 75% and 71% in the prediction of disease recurrence. Patients' age and substage were of no prognostic value. We conclude that the nuclear morphometric characteristics, especially the presence of giant nuclear cells and nuclear irregularity, may be useful in predicting outcome in patients with early stage ovarian clear cell adenocarcinoma. © 1999 Cancer Research Campaign

5-methylcytosine (m5C) as a rare base exists in eukaryotic genomes, which is a normal constitution in many eukaryotic DNA and the existence of m5C is a feature of eukaryotic DNA. Under regular physiological conditions, cytosine of eukaryotic DNA is usually methylated. Up to the present, many people consider that the m5C may be mutation hotspots by the deamination leading to gene mutation. Our study indicated that the spontaneous mutation caused by the transition of G.C --> A.T, in eukaryotic DNA, may result from the tautomer changing of base pairs and may also be cause by other factor actions, however it could not be caused by the deamination of m5C.