1. Background suppression in fluorescence nanoscopy with stimulated emission double depletion
- Author
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Peng Gao, G. Ulrich Nienhaus, Benedikt Prunsche, Lu Zhou, and Karin Nienhaus
- Subjects
0301 basic medicine ,Physics ,Photon ,Super-resolution microscopy ,Pulse (signal processing) ,business.industry ,STED microscopy ,Nanotechnology ,Fluorescence correlation spectroscopy ,computer.software_genre ,Fluorescence ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,03 medical and health sciences ,030104 developmental biology ,Optics ,Voxel ,Stimulated emission ,business ,computer - Abstract
Stimulated emission depletion (STED) fluorescence nanoscopy is a powerful super-resolution imaging technique based on the confinement of fluorescence emission to the central subregion of an observation volume through de-excitation of fluorophores in the periphery via stimulated emission. Here, we introduce stimulated emission double depletion (STEDD) as a method to selectively remove artificial background intensity. In this approach, a first, conventional STED pulse is followed by a second, delayed Gaussian STED pulse that specifically depletes the central region, thus leaving only background. Thanks to time-resolved detection we can remove this background intensity voxel by voxel by taking the weighted difference of photons collected before and after the second STED pulse. STEDD thus yields background-suppressed super-resolved images as well as STED-based fluorescence correlation spectroscopy data. Furthermore, the proposed method is also beneficial when considering lower-power, less redshifted depletion pulses. Stimulated emission double depletion addresses the issue of background in super-resolution imaging and quantitative microscopy through implementation of a two-pulse sequence in a modified stimulated emission depletion set-up. The measured background intensity is removed from each voxel in the acquired images thanks to time-resolved detection.
- Published
- 2017
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