1. CD8 serum levels in acute graft-versus-host disease diagnosis
- Author
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Angrilli F, P. Di Bartolomeo, Angelini A, Torlontano G, P Bavaro, G. Papalinetti, T. Bonfini, G. Di Girolamo, and P Olioso
- Subjects
Adult ,Male ,medicine.medical_specialty ,Time Factors ,Adolescent ,Bone marrow transplantation ,CD8 Antigens ,Immunology ,Graft vs Host Disease ,Gastroenterology ,Immunopathology ,Internal medicine ,Acute graft versus host disease ,medicine ,Humans ,Immunology and Allergy ,In patient ,Bone Marrow Transplantation ,business.industry ,Middle Aged ,Surgery ,Transplantation ,medicine.anatomical_structure ,Child, Preschool ,Acute Disease ,CD4 Antigens ,T cell subset ,Female ,Bone marrow ,business ,CD8 - Abstract
Attempts to identify an early and discriminating marker of acute graft-versus-host disease (aGvHD) have been unsuccessful. The levels of soluble CD4 and soluble CD8 in serum correlate with T cell subset activation and may be important in monitoring and characterizing immunological processes. We determined serum soluble CD4 (sCD4) and sCD8 levels with a two-site sandwich enzyme immunoassay on patients' serum samples collected prior to bone marrow transplantation and weekly after transplantation until day +28. No significant increment of sCD4 was documented in each determination. sCD8 rose significantly before diagnosis or development of maximal clinical symptoms in patients with grade II-III aGvHD than grade 0-I aGvHD [at day +21--median value 447 IU/ml; range 94-713; versus 1136 IU/ml, range 790-1416 (P = 0.002); at day +28--median value 443 IU/ml, range 73-992, versus 1164 IU/ml, range 625-1960 (P = 0.005)]. On the day of marrow infusion the sCD8 levels were significantly higher in patients who subsequently developed grade II-III than in patients with grade 0-I aGvHD (median value 155 IU/ml, range 10-332, versus 350 IU/ml, range 283-830; P = 0.003). Careful monitoring of sCD8 is a useful tool for a prompt aGvHD diagnosis and may be used in a clinical bone marrow transplantation setting.
- Published
- 1994
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