1. Noninvasive diagnostic criteria for nonalcoholic steatohepatitis based on gene expression levels in peripheral blood mononuclear cells
- Author
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Hidetaka Fujinaga, Kazuhiko Ikeuchi, Kazuya Okushin, Takeya Tsutsumi, Kenichiro Enooku, Hiroshi Yotsuyanagi, Kyoji Moriya, Akira Kado, and Kazuhiko Koike
- Subjects
Adult ,Male ,Cirrhosis ,digestive system ,Peripheral blood mononuclear cell ,03 medical and health sciences ,0302 clinical medicine ,Non-alcoholic Fatty Liver Disease ,Nonalcoholic fatty liver disease ,medicine ,Humans ,Aged ,medicine.diagnostic_test ,business.industry ,Fatty liver ,Gastroenterology ,nutritional and metabolic diseases ,Middle Aged ,medicine.disease ,digestive system diseases ,Interleukin 10 ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,Hepatocellular carcinoma ,Liver biopsy ,Immunology ,Disease Progression ,Leukocytes, Mononuclear ,Cytokines ,Female ,030211 gastroenterology & hepatology ,Chemokines ,Steatosis ,business - Abstract
Nonalcoholic fatty liver disease (NAFLD) consists of nonalcoholic fatty liver (NAFL) and nonalcoholic steatohepatitis (NASH); the latter progresses to liver cirrhosis and hepatocellular carcinoma. Discriminating NASH from NAFL typically involves liver biopsy. The mechanism of NASH progression is unclear but may involve immunological pathways. In this study, we examined expression levels of cytokine- and chemokine-encoding genes in peripheral blood mononuclear cells (PBMCs) from NAFLD patients and established immunological criteria for discriminating NASH from NAFL. PBMCs were obtained from 54 patients diagnosed histologically with NAFLD (NAFL, 18; NASH, 36). mRNA was extracted from PBMCs, and expression levels of cytokine- and chemokine-encoding genes were determined by quantitative real-time PCR. Statistical analysis was performed by nonparametric test. Expression levels of interferon (IFN)γ, interleukin (IL)2, IL15, C–C-motif chemokine ligand (CCL)2, IL10, and C-X-C-motif chemokine ligand (CXCL)11 were significantly upregulated in NASH patients compared with NAFL patients. Moreover, their expression levels were positively correlated with the degree of ballooning of hepatocytes but not of steatosis or lobular inflammation. We focused on those encoding IL10, IFNγ, and CCL2, and developed a scoring system to discriminate NASH from NAFL. The discriminatory power of the criteria was validated in an independent cohort. Expression levels of the cytokine- and chemokine-encoding genes in PBMCs were positively correlated with ballooning, suggesting their utility for the diagnosis of NASH. The data indicate that peripheral as well as intrahepatic immunity is involved in the progression of NASH. Our findings afford new insight into immunological mechanisms of NASH and will facilitate its noninvasive diagnosis.
- Published
- 2019