1. Defining the combined stress response in wild Arachis
- Author
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Ana Cristina Miranda Brasileiro, Maria Fatima Grossi-de-Sa, Mario Alfredo de Passos Saraiva, Ana Paula Zotta Mota, Bruna Vidigal, R. C. Togawa, Patricia Messenberg Guimaraes, Andressa da Cunha Quintana Martins, Thais Nicolini Oliveira, Ana Claudia Guerra de Araújo, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Embrapa Recursos Genéticos e Biotecnologia [Brasília], INCT Plant Stress Biotech, Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), UCB Pharma SA, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Apoio a Pesquisa do Distrito Federal (FAPDF), ANA PAULA ZOTTA MOTA, UFRGS, ANA CRISTINA MIRANDA BRASILEIRO, Cenargen, BRUNA VIDIGAL, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, THAIS NICOLINI OLIVEIRA, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, ANDRESSA DA CUNHA QUINTANA MARTINS, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, MARIO ALFREDO DE PASSOS SARAIVA, Cenargen, ANA CLAUDIA GUERRA DE ARAUJO, Cenargen, ROBERTO COITI TOGAWA, Cenargen, MARIA FATIMA GROSSI DE SA, Cenargen, and PATRICIA MESSEMBERG GUIMARAES, Cenargen.
- Subjects
0106 biological sciences ,0301 basic medicine ,Meloidogyne Arenaria ,Candidate gene ,Arachis ,[SDV]Life Sciences [q-bio] ,Science ,Molecular engineering in plants ,Wild Arachis ,01 natural sciences ,Article ,Transcriptome ,03 medical and health sciences ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Stress, Physiological ,Arabidopsis ,Animals ,Tylenchoidea ,Transcriptomics ,Biotic ,Abscisic acid ,Gene ,Disease Resistance ,Plant Diseases ,2. Zero hunger ,Genetics ,Abiotic component ,Multidisciplinary ,Abiotic ,biology ,fungi ,food and beverages ,biology.organism_classification ,Droughts ,030104 developmental biology ,chemistry ,Meloidogyne arenaria ,Medicine ,Ethylene hormone ,010606 plant biology & botany - Abstract
Nematodes and drought are major constraints in tropical agriculture and often occur simultaneously. Plant responses to these stresses are complex and require crosstalk between biotic and abiotic signaling pathways. In this study, we explored the transcriptome data of wild Arachis species subjected to drought (A-metaDEG) and the root-knot nematode Meloidogyne arenaria (B-metaDEG) via meta-analysis, to identify core-stress responsive genes to each individual and concurrent stresses in these species. Transcriptome analysis of a nematode/drought bioassay (cross-stress) showed that the set of stress responsive DEGs to concurrent stress is distinct from those resulting from overlapping A- and B-metaDEGs, indicating a specialized and unique response to combined stresses in wild Arachis. Whilst individual biotic and abiotic stresses elicit hormone-responsive genes, most notably in the jasmonic and abscisic acid pathways, combined stresses seem to trigger mainly the ethylene hormone pathway. The overexpression of a cross-stress tolerance candidate gene identified here, an endochitinase-encoding gene (AsECHI) from Arachis stenosperma, reduced up to 30% of M. incognita infection and increased post-drought recovery in Arabidopsis plants submitted to both stresses. The elucidation of the network of cross-stress responsive genes in Arachis contributes to better understanding the complex regulation of biotic and abiotic responses in plants facilitating more adequate crop breeding for combined stress tolerance.
- Published
- 2021
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