1. Antithrombotic Activity of Brewers’ Spent Grain Peptides and their Effects on Blood Coagulation Pathways
- Author
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Cecilia C. Botto, Olga Martínez-Augustin, Antonela G. Garzón, Raúl E. Cian, and Silvina R. Drago
- Subjects
0301 basic medicine ,ACE I AND Α-AMYLASE INHIBITION MECHANISM ,Otras Ingenierías y Tecnologías ,Protein Hydrolysates ,Thrombin Time ,Angiotensin-Converting Enzyme Inhibitors ,INGENIERÍAS Y TECNOLOGÍAS ,Hydrolysate ,Alimentos y Bebidas ,BREWERSundefined SPENT GRAIN ,03 medical and health sciences ,0404 agricultural biotechnology ,Thrombin ,Fibrinolytic Agents ,medicine ,Thromboplastin ,IN VITRO GASTROINTESTINAL DIGESTION ,Blood Coagulation ,Plant Proteins ,chemistry.chemical_classification ,030109 nutrition & dietetics ,Molecular mass ,MULTI-ENZYME BIOACTIVE PEPTIDE EXTRACTION ,04 agricultural and veterinary sciences ,040401 food science ,Amino acid ,Gastrointestinal Tract ,Ultrafiltration (renal) ,Enzyme ,chemistry ,Clotting time ,Biochemistry ,Chemistry (miscellaneous) ,ANTITHROMBOTIC PEPTIDES ,Digestion ,Edible Grain ,Peptides ,Food Science ,medicine.drug - Abstract
Antithrombotic activity of brewers’ spent grain peptides before and after simulated gastrointestinal digestion and their effects on blood coagulation pathways were evaluated. Two hydrolysates were produced using sequential enzymatic systems: alkaline protease + Flavourzyme (AF) and neutral protease + Flavourzyme (PF). Simulation of gastrointestinal digestion of AF and PF hydrolysates was made using porcine pepsin and pancreatin enzymes, obtaining the corresponding digested samples: AFD and PFD, respectively. Peptides were fractionated by ultrafiltration using a 1 kDa cut-off membrane. Hydrolysates had peptides with medium and low molecular weights (2100 and 500 Da, respectively), and Glu, Asp, Leu, Ala, and Phe were the most abundant amino acids. Gastrointestinal digested hydrolysates presented high proportion of small peptides (~500 Da), and higher amount of Val, Tyr, and Phe than hydrolysates. Mass spectrum (HDMS Q-TOF) of AFD-ultrafiltered fraction
- Published
- 2018
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