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1. Impact of SARS-CoV-2 spike stability and RBD exposure on antigenicity and immunogenicity

Author

Rutten, Lucy, primary, Swart, Maarten, additional, Koornneef, Annemart, additional, Bouchier, Pascale, additional, Blokland, Sven, additional, Sadi, Ava, additional, Juraszek, Jarek, additional, Vijayan, Aneesh, additional, Schmit-Tillemans, Sonja, additional, Verspuij, Johan, additional, Choi, Ying, additional, Daal, Chenandly E., additional, Perkasa, Aditya, additional, Torres Morales, Shessy, additional, Myeni, Sebenzile K., additional, Kikkert, Marjolein, additional, Tolboom, Jeroen, additional, van Manen, Daniëlle, additional, Kuipers, Harmjan, additional, Schuitemaker, Hanneke, additional, Zahn, Roland, additional, and Langedijk, Johannes P. M., additional

Published
2024
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3. Nicotine Replacement Therapy Does Not Reduce Headaches Following Subarachnoid Hemorrhage: A Propensity Score-Matched Study

Author

Aude Charvet, Baptiste Bouchier, Frédéric Dailler, and Thomas Ritzenthaler

Subjects
Neurology (clinical), Critical Care and Intensive Care Medicine
Abstract

A significant number of patients admitted for aneurysmal subarachnoid hemorrhage (aSAH) are active smokers and are at risk of developing nicotine withdrawal symptoms (e.g., cravings, irritability, insomnia, headaches, etc.). This study aimed to evaluate the use of nicotine replacement therapy (NRT) regarding headache severity and analgesics consumption.A retrospective study was conducted using prospectively collected data from 2014 to 2019 in the neurointensive care unit of the Hospices Civils in Lyon, France. We performed a propensity score matching analysis. The covariables used were age, sex, initial World Federation of Neurosurgical Societies score, Hijdra sum score, and factors associated with pain following aSAH (history of chronic pain, anxiety, or depression). Smokers received NRT through a transdermal device. The primary end point was headache control. Secondary end points were mean numerical pain rating scale score and analgesics consumption.One hundred and fifty-five patients were included among 523 patients hospitalized for aSAH. Fifty-one patients underwent nicotine substitution and were matched to 51 unsubstituted patients. The headache control rate was not different between the two groups (43.1% vs. 31.4%, p = 0.736). The mean numeric pain rating scale score in the substituted group was 2.2 (1.1-3.5) and 2.4 (1.6-3.1) in the unsubstituted group (p = 0.533). The analgesics consumption (acetaminophen, tramadol, and morphine) was the same in the two groups.The use of NRT in the acute phase of aSAH does not seem to have an impact on the intensity of headaches or analgesics consumption.

Published
2022
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5. Caspase-2 regulates S-phase cell cycle events to protect from DNA damage accumulation independent of apoptosis

Author

Vijay Charaka, Alexandre F. Carisey, Ashley Boice, Raj K. Pandita, Tej K. Pandita, Lisa Bouchier-Hayes, Karla E. Lopez, Melissa J. Parsons, and Chloé I. Charendoff

Subjects
Cancer Research, Programmed cell death, Cell cycle checkpoint, Cell division, DNA repair, DNA damage, Genetics, DNA replication, Biology, Cell cycle, Molecular Biology, Cell biology, Replication fork protection
Abstract

In addition to its classical role in apoptosis, accumulating evidence suggests that caspase-2 has non-apoptotic functions, including regulation of cell division. Loss of caspase-2 is known to increase proliferation rates but how caspase-2 is regulating this process is currently unclear. We show that caspase-2 is activated in dividing cells in G1-phase of the cell cycle. In the absence of caspase-2, cells exhibit numerous S-phase defects including delayed exit from S-phase, defects in repair of chromosomal aberrations during S-phase, and increased DNA damage following S-phase arrest. In addition, caspase-2-deficient cells have a higher frequency of stalled replication forks, decreased DNA fiber length, and impeded progression of DNA replication tracts. This indicates that caspase-2 protects from replication stress and promotes replication fork protection to maintain genomic stability. These functions are independent of the pro-apoptotic function of caspase-2 because blocking caspase-2-induced cell death had no effect on cell division, DNA damage-induced cell cycle arrest, or DNA damage. Thus, our data supports a model where caspase-2 regulates cell cycle and DNA repair events to protect from the accumulation of DNA damage independently of its pro-apoptotic function.

Published
2021
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8. Low CCR5 expression protects HIV-specific CD4+ T cells of elite controllers from viral entry

Author

Claireaux, Mathieu, primary, Robinot, Rémy, additional, Kervevan, Jérôme, additional, Patgaonkar, Mandar, additional, Staropoli, Isabelle, additional, Brelot, Anne, additional, Nouël, Alexandre, additional, Gellenoncourt, Stacy, additional, Tang, Xian, additional, Héry, Mélanie, additional, Volant, Stevenn, additional, Perthame, Emeline, additional, Avettand-Fenoël, Véronique, additional, Buchrieser, Julian, additional, Cokelaer, Thomas, additional, Bouchier, Christiane, additional, Ma, Laurence, additional, Boufassa, Faroudy, additional, Hendou, Samia, additional, Libri, Valentina, additional, Hasan, Milena, additional, Zucman, David, additional, de Truchis, Pierre, additional, Schwartz, Olivier, additional, Lambotte, Olivier, additional, and Chakrabarti, Lisa A., additional

Published
2022
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10. Stabilizing the closed SARS-CoV-2 spike trimer

Author

Juraszek, Jarek, primary, Rutten, Lucy, additional, Blokland, Sven, additional, Bouchier, Pascale, additional, Voorzaat, Richard, additional, Ritschel, Tina, additional, Bakkers, Mark J. G., additional, Renault, Ludovic L. R., additional, and Langedijk, Johannes P. M., additional

Published
2021
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12. TbD1 deletion as a driver of the evolutionary success of modern epidemic Mycobacterium tuberculosis lineages

Author

Bottai, Daria, primary, Frigui, Wafa, additional, Sayes, Fadel, additional, Di Luca, Mariagrazia, additional, Spadoni, Dalila, additional, Pawlik, Alexandre, additional, Zoppo, Marina, additional, Orgeur, Mickael, additional, Khanna, Varun, additional, Hardy, David, additional, Mangenot, Sophie, additional, Barbe, Valerie, additional, Medigue, Claudine, additional, Ma, Laurence, additional, Bouchier, Christiane, additional, Tavanti, Arianna, additional, Larrouy-Maumus, Gerald, additional, and Brosch, Roland, additional

Published
2020
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16. Publisher Correction: Genomic insights into the 2016–2017 cholera epidemic in Yemen

Author

Weill, François-Xavier, primary, Domman, Daryl, additional, Njamkepo, Elisabeth, additional, Almesbahi, Abdullrahman A., additional, Naji, Mona, additional, Nasher, Samar Saeed, additional, Rakesh, Ankur, additional, Assiri, Abdullah M., additional, Sharma, Naresh Chand, additional, Kariuki, Samuel, additional, Pourshafie, Mohammad Reza, additional, Rauzier, Jean, additional, Abubakar, Abdinasir, additional, Carter, Jane Y., additional, Wamala, Joseph F., additional, Seguin, Caroline, additional, Bouchier, Christiane, additional, Malliavin, Thérèse, additional, Bakhshi, Bita, additional, Abulmaali, Hayder H. N., additional, Kumar, Dhirendra, additional, Njoroge, Samuel M., additional, Malik, Mamunur Rahman, additional, Kiiru, John, additional, Luquero, Francisco J., additional, Azman, Andrew S., additional, Ramamurthy, Thandavarayan, additional, Thomson, Nicholas R., additional, and Quilici, Marie-Laure, additional

Published
2019
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17. Twitter expands the reach and engagement of a national scientific meeting: the Irish Society of Urology

Author

Gregory J. Nason, D. Bouchier-Hayes, F. O’Kelly, Rustom P. Manecksha, and David M. Quinlan

Subjects
Internet, medicine.medical_specialty, Blogging, Information Dissemination, business.industry, Communication, Interprofessional Relations, Urology, General Medicine, Congresses as Topic, Research Personnel, language.human_language, Irish, Analytics, Physicians, language, Humans, Medicine, Social media, business, Cyberspace, Ireland, Social Media, Healthcare analytics
Abstract

Social media is the interaction among people in which they create, share or exchange information and ideas in virtual communities and web-based networks. This year, the Irish Society of Urology (ISU) expanded its involvement in social media with a preregistered Twitter hashtag (#ISU14) for the annual meeting. The aim of this study was to highlight the use of Twitter at an annual national meeting held in 2014. The Symplur healthcare analytics website was used to prospectively examine traffic related to the 2014 ISU Annual Meeting. This feature was used to generate statistics for the number of impressions, unique tweets (excluding retweets) and distinct contributors who used the indexing hashtag #ISU14. Individual tweets were assessed using the conference hashtag on the Twitter website. The total number of attendees at the conference was 119, and 99 individuals participated in Twitter using the conference hashtag (#ISU14). 31 % of attendees participated in tweeting at the conference. Over the course of the conference, a total of 798 unique tweets were generated, creating over 665,000 impressions in cyberspace. 590 (73.9 %) tweets were generated from attendees at the conference, while 26.1 % of tweets were from virtual followers. 702 (87.9 %) tweets were from urologists and 439 (55 %) tweets were of scientific nature. Tweet activity peaked during the guest lectures on both days. Twitter use at the ISU has been shown to facilitate interaction between delegates and allows users to follow as well as participate from afar.

Published
2015
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18. A novel species of the marine cyanobacterium Acaryochloris with a unique pigment content and

Author

Partensky, F., Six, C., Ratin, M., Garczarek, L., Vaulot, D. (Daniel), Probert, I., Calteau, A., Gourvil, P., Marie, D., Grébert, T., Bouchier, C., Le Panse C. C., Gachenot, M., Rodríguez, F. (Francisco), and Garrido, J.L.

Abstract

All characterized members of the ubiquitous genus Acaryochloris share the unique property of containing large amounts of chlorophyll (Chl) d, a pigment exhibiting a red absorption maximum strongly shifted towards infrared compared to Chl a. Chl d is the major pigment in these organisms and is notably bound to antenna proteins structurally similar to those of Prochloron, Prochlorothrix and Prochlorococcus, the only three cyanobacteria known so far to contain mono- or divinyl-Chl a and b as major pigments and to lack phycobilisomes. Here, we describe RCC1774, a strain isolated from the foreshore near Roscof (France). It is phylogenetically related to members of the Acaryochloris genus but completely lacks Chl d. Instead, it possesses monovinyl-Chl a and b at a b/a molar ratio of 0.16, similar to that in Prochloron and Prochlorothrix. It difers from the latter by the presence of phycocyanin and a vestigial allophycocyanin energetically coupled to photosystems. Genome sequencing confrmed the presence of phycobiliprotein and Chl b synthesis genes. Based on its phylogeny, ultrastructural characteristics and unique pigment suite, we describe RCC1774 as a novel species that we name Acaryochloris thomasi. Its very unusual pigment content compared to other Acaryochloris spp. is likely related to its specifc lifestyle.

Published
2018

20. Publisher Correction: Genomic insights into the 2016–2017 cholera epidemic in Yemen

Author

Mona Naji, Daryl Domman, Abdinasir Abubakar, Joseph F. Wamala, Hayder H.N. Abulmaali, Mohammad Reza Pourshafie, Elisabeth Njamkepo, Naresh Chand Sharma, Ankur Rakesh, François-Xavier Weill, Bita Bakhshi, Andrew S. Azman, Samuel M. C. Njoroge, Thérèse E. Malliavin, Samuel Kariuki, Abdullrahman A. Almesbahi, Jane Y. Carter, Mamunur Rahman Malik, Jean Rauzier, Francisco J. Luquero, Marie Laure Quilici, Abdullah M. Assiri, Thandavarayan Ramamurthy, Samar Saeed Nasher, Christiane Bouchier, Caroline Seguin, John Kiiru, Nicholas R. Thomson, and Dhirendra Kumar

Subjects
0303 health sciences, 03 medical and health sciences, Multidisciplinary, History, 030306 microbiology, ComputingMethodologies_DOCUMENTANDTEXTPROCESSING, medicine, medicine.disease, Cholera, Genealogy, 030304 developmental biology
Abstract

In the HTML version of this Letter, the affiliations for authors Andrew S. Azman, Dhirendra Kumar and Thandavarayan Ramamurthy were inverted (the PDF and print versions of the Letter were correct); the affiliations have been corrected online.

Published
2019
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21. Genomic insights into the 2016–2017 cholera epidemic in Yemen

Author

Weill, François-Xavier, primary, Domman, Daryl, additional, Njamkepo, Elisabeth, additional, Almesbahi, Abdullrahman A., additional, Naji, Mona, additional, Nasher, Samar Saeed, additional, Rakesh, Ankur, additional, Assiri, Abdullah M., additional, Sharma, Naresh Chand, additional, Kariuki, Samuel, additional, Pourshafie, Mohammad Reza, additional, Rauzier, Jean, additional, Abubakar, Abdinasir, additional, Carter, Jane Y., additional, Wamala, Joseph F., additional, Seguin, Caroline, additional, Bouchier, Christiane, additional, Malliavin, Thérèse, additional, Bakhshi, Bita, additional, Abulmaali, Hayder H. N., additional, Kumar, Dhirendra, additional, Njoroge, Samuel M., additional, Malik, Mamunur Rahman, additional, Kiiru, John, additional, Luquero, Francisco J., additional, Azman, Andrew S., additional, Ramamurthy, Thandavarayan, additional, Thomson, Nicholas R., additional, and Quilici, Marie-Laure, additional

Published
2019
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22. A novel species of the marine cyanobacterium Acaryochloris with a unique pigment content and lifestyle

Author

Partensky, Frédéric, primary, Six, Christophe, additional, Ratin, Morgane, additional, Garczarek, Laurence, additional, Vaulot, Daniel, additional, Probert, Ian, additional, Calteau, Alexandra, additional, Gourvil, Priscillia, additional, Marie, Dominique, additional, Grébert, Théophile, additional, Bouchier, Christiane, additional, Le Panse, Sophie, additional, Gachenot, Martin, additional, Rodríguez, Francisco, additional, and Garrido, José L., additional

Published
2018
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23. Gene flow contributes to diversification of the major fungal pathogen Candida albicans

Author

Ropars, Jeanne, primary, Maufrais, Corinne, additional, Diogo, Dorothée, additional, Marcet-Houben, Marina, additional, Perin, Aurélie, additional, Sertour, Natacha, additional, Mosca, Kevin, additional, Permal, Emmanuelle, additional, Laval, Guillaume, additional, Bouchier, Christiane, additional, Ma, Laurence, additional, Schwartz, Katja, additional, Voelz, Kerstin, additional, May, Robin C., additional, Poulain, Julie, additional, Battail, Christophe, additional, Wincker, Patrick, additional, Borman, Andrew M., additional, Chowdhary, Anuradha, additional, Fan, Shangrong, additional, Kim, Soo Hyun, additional, Le Pape, Patrice, additional, Romeo, Orazio, additional, Shin, Jong Hee, additional, Gabaldon, Toni, additional, Sherlock, Gavin, additional, Bougnoux, Marie-Elisabeth, additional, and d’Enfert, Christophe, additional

Published
2018
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25. Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome

Author

Dickson, Laura B., primary, Ghozlane, Amine, additional, Volant, Stevenn, additional, Bouchier, Christiane, additional, Ma, Laurence, additional, Vega-Rúa, Anubis, additional, Dusfour, Isabelle, additional, Jiolle, Davy, additional, Paupy, Christophe, additional, Mayanja, Martin N., additional, Kohl, Alain, additional, Lutwama, Julius J., additional, Duong, Veasna, additional, and Lambrechts, Louis, additional

Published
2018
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26. Distinct lineages of Ebola virus in Guinea during the 2014 West African epidemic

Author

Laure Diancourt, Etienne Simon-Loriere, Edward C. Holmes, Jan P. Buchmann, Gamou Fall, Matthias Vandenbogaert, Christiane Bouchier, Valérie Caro, Christian B. Matranga, N’Faly Magassouba, Oumar Faye, Sakoba Keita, Ousmane Faye, Jean-Michel Thiberge, Lamine Koivogui, Pardis C. Sabeti, Amadou A. Sall, Jean-Claude Manuguerra, Génétique fonctionnelle des Maladies infectieuses - Functional Genetics of Infectious Diseases, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Arbovirus et Virus de Fièvres Hémorragiques [Dakar, Sénégal], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Institut National de Santé Publique [Conakry, Guinée] (INSP), Ministère de la Santé [Conakry, Guinea], Université Gamal Abdel Nasser de Conakry, Ministry of Health [Guinée], Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats (CIBU), Institut Pasteur [Paris] (IP), Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Génomique (Plate-Forme) - Genomics Platform, Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), The University of Sydney, Broad Institute [Cambridge], Harvard University-Massachusetts Institute of Technology (MIT), Harvard University, This study was supported by the Pasteur Ebola Task Force (PETF) and has also received funding from the French government’s Investissement d’Avenir programme, Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (grant number ANR-10-LABX-62-IBEID) and Institut Pasteur de Dakar. We are grateful to all members of the PETF for their support, and in particular F. Rey and K. Victoir. High-throughput sequencing was performed on the Genomics Platform of Institut Pasteur, member of ‘France Génomique’ consortium (ANR10-INBS-09-08)., We thank L. Ma for technical assistance and Institut Pasteur Clinical Research Department for their help with ethical approval procedures. E.C.H. is supported by an NHMRC Australia fellowship., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), and ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010)

Subjects
Glycosylation, Lineage (genetic), [SDV]Life Sciences [q-bio], Molecular Sequence Data, Genome, Viral, Biology, Mali, medicine.disease_cause, Virus, Sierra Leone, Sierra leone, Evolution, Molecular, Phylogenetics, medicine, Humans, Phylogeny, Glycoproteins, Multidisciplinary, Ebola virus, Phylogenetic tree, Transmission (medicine), Viral Core Proteins, Mucins, Genetic Variation, Outbreak, Hemorrhagic Fever, Ebola, Nucleocapsid Proteins, Ebolavirus, RNA-Dependent RNA Polymerase, Virology, Protein Structure, Tertiary, Nucleoproteins, Mutation, Guinea
Abstract

International audience; An epidemic of Ebola virus disease of unprecedented scale has been ongoing for more than a year in West Africa. As of 29 April 2015, there have been 26,277 reported total cases (of which 14,895 have been laboratory confirmed) resulting in 10,899 deaths. The source of the outbreak was traced to the prefecture of Guéckédou in the forested region of southeastern Guinea. The virus later spread to the capital, Conakry, and to the neighbouring countries of Sierra Leone, Liberia, Nigeria, Senegal and Mali. In March 2014, when the first cases were detected in Conakry, the Institut Pasteur of Dakar, Senegal, deployed a mobile laboratory in Donka hospital to provide diagnostic services to the greater Conakry urban area and other regions of Guinea. Through this process we sampled 85 Ebola viruses (EBOV) from patients infected from July to November 2014, and report their full genome sequences here. Phylogenetic analysis reveals the sustained transmission of three distinct viral lineages co-circulating in Guinea, including the urban setting of Conakry and its surroundings. One lineage is unique to Guinea and closely related to the earliest sampled viruses of the epidemic. A second lineage contains viruses probably reintroduced from neighbouring Sierra Leone on multiple occasions, while a third lineage later spread from Guinea to Mali. Each lineage is defined by multiple mutations, including non-synonymous changes in the virion protein 35 (VP35), glycoprotein (GP) and RNA-dependent RNA polymerase (L) proteins. The viral GP is characterized by a glycosylation site modification and mutations in the mucin-like domain that could modify the outer shape of the virion. These data illustrate the ongoing ability of EBOV to develop lineage-specific and potentially phenotypically important variation.

Published
2015
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27. Genetic deletion of caspase-2 accelerates MMTV/c-neu-driven mammary carcinogenesis in mice

Author

Melissa J. Parsons, Laura L. McCormick, Laura J. Janke, Lisa Bouchier-Hayes, A Howard, and Douglas R. Green

Subjects
rho GTP-Binding Proteins, Death Domain Receptor Signaling Adaptor Proteins, animal structures, Carcinogenesis, viruses, Caspase 2, Mammary Neoplasms, Animal, medicine.disease_cause, Mice, Mammary tumor virus, medicine, Animals, Molecular Biology, Cells, Cultured, Caspase, Cell Proliferation, Mice, Knockout, Original Paper, biology, Cell growth, Tumor Suppressor Proteins, Cell Cycle, Proto-Oncogene Proteins c-mdm2, Cell Biology, Cell cycle, Molecular biology, Cell Transformation, Neoplastic, Mammary Tumor Virus, Mouse, Apoptosis, biology.protein, Mdm2, Female, Tumor Suppressor Protein p53
Abstract

Despite being the most evolutionarily conserved of the mammalian caspases, little is understood about the cellular function of caspase-2 in normal tissues or what role caspase-2 may have in the progression of human disease. It has been reported that deletion of the caspase-2 gene (Casp2), accelerates Eμ-myc lymphomagenesis in mice, and thus caspase-2 may act as a tumor suppressor in hematological malignancies. Here, we sought to extend these findings to epithelial cancers by examining the potential role of caspase-2 as a tumor suppressor in the mouse mammary carcinogenesis model; MMTV/c-neu. The rate of tumor acquisition was significantly higher in multiparous Casp2(-/-)/MMTV mice compared with Casp2(+/+)/MMTV and Casp2(+/-)/MMTV mice. Cells from Casp2(-/-)/MMTV tumors were often multinucleated and displayed bizarre mitoses and karyomegaly, while cells from Casp2(+/+)/MMTV and Casp2(+/-)/MMTV tumors never displayed this phenotype. Tumors from Casp2(-/-)/MMTV animals had a significantly higher mitotic index than tumors from Casp2(+/+)/MMTV and Casp2(+/-)/MMTV animals. Cell cycle analysis of Casp2(-/-) E1A/Ras-transformed mouse embryonic fibroblasts (MEF) also indicated a higher proliferative rate in the absence of caspase-2. In vitro assays further illustrated that MEF had increased genomic instability in the absence of caspase-2. This appears to be due to disruption of the p53 pathway because we observed a concomitant decrease in the induction of the p53 target genes, Pidd, p21 and Mdm2. Thus caspase-2 may function as a tumor suppressor, in part, through regulation of cell division and genomic stability.

Published
2013
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28. Genomic analysis of smooth tubercle bacilli provides insights into ancestry and pathoadaptation of Mycobacterium tuberculosis

Author

Wafa Frigui, Fadel Sayes, Camille Locht, Gregory Salvignol, Varun Khanna, Eve Willery, Danielle Walker, Anne-Sophie Debrie, Philip Supply, Michael Marceau, Timothy P. Stinear, Alessandro Cascioferro, Roland Brosch, Sylvain Brisse, Michael A. Quail, Stephen D. Bentley, Michel Fabre, Sophie Mangenot, Laurence Fiette, Claudine Médigue, Stéphane Cruveiller, Carine Rouanet, Claude Leclerc, Alexandre Pawlik, Julien Tap, Torsten Seemann, Laleh Majlessi, Christiane Bouchier, Roxane Simeone, Laurence Ma, David Roche, Cecile Parmentier, Valérie Barbe, Mickael Orgeur, Julian Parkhill, Eva C. Boritsch, Alexis Criscuolo, Maria-Cristina Gutierrez, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Pathogénomique mycobactérienne intégrée, Institut Pasteur [Paris] (IP), Régulation Immunitaire et Vaccinologie, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Génotypage des Pathogènes et Santé Publique (Plate-forme) (PF8), Histopathologie humaine et Modèles animaux, Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), HIA Percy, Mécanismes moléculaires de la pathogénie microbienne, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM), The Wellcome Trust Sanger Institute [Cambridge], Génomique (Plate-Forme) - Genomics Platform, Victorian Bioinformatics Consortium [Clayton], Monash University [Clayton], Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Mécanismes moléculaires de la pathogenèse bactérienne, Département Infection et Epidémiologie - Department of Infection and Epidemiology, University of Melbourne, The work was supported in part by the Institut Pasteur (PTR 314 and PTR 383), European Community's Framework Programme 7 grant 260872, Wellcome Trust grant 098051 and Genoscope collaborative grant 114., European Project: 260872,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,MM4TB(2011), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)

Subjects
MESH: Sequence Analysis, DNA, MESH: Mycobacterium tuberculosis, Tuberculosis, MESH: Spleen, [SDV]Life Sciences [q-bio], MESH: Mice, Inbred BALB C, Virulence, Genomics, MESH: Virulence, MESH: Base Sequence, MESH: Genome, Bacterial, Genome, DNA sequencing, Mycobacterium tuberculosis, 03 medical and health sciences, Genetic variation, Genetics, medicine, MESH: Species Specificity, MESH: Animals, MESH: Lung, MESH: Mice, 030304 developmental biology, 0303 health sciences, MESH: Molecular Sequence Data, biology, 030306 microbiology, MESH: Adaptation, Biological, MESH: Genomics, MESH: Polymorphism, Single Nucleotide, biology.organism_classification, medicine.disease, MESH: Cluster Analysis, 3. Good health, MESH: Inverted Repeat Sequences, Mycobacterium
Abstract

International audience; Global spread and limited genetic variation are hallmarks of M. tuberculosis, the agent of human tuberculosis. In contrast, Mycobacterium canettii and related tubercle bacilli that also cause human tuberculosis and exhibit unusual smooth colony morphology are restricted to East Africa. Here, we sequenced and analyzed the whole genomes of five representative strains of smooth tubercle bacilli (STB) using Sanger (4-5× coverage), 454/Roche (13-18× coverage) and/or Illumina DNA sequencing (45-105× coverage). We show that STB isolates are highly recombinogenic and evolutionarily early branching, with larger genome sizes, higher rates of genetic variation, fewer molecular scars and distinct CRISPR-Cas systems relative to M. tuberculosis. Despite the differences, all tuberculosis-causing mycobacteria share a highly conserved core genome. Mouse infection experiments showed that STB strains are less persistent and virulent than M. tuberculosis. We conclude that M. tuberculosis emerged from an ancestral STB-like pool of mycobacteria by gain of persistence and virulence mechanisms, and we provide insights into the molecular events involved.

Published
2013
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29. Erratum: Global phylogeography and evolutionary history of Shigella dysenteriae type 1

Author

Elisabeth Njamkepo, Nizar Fawal, Alicia Tran-Dien, Jane Hawkey, Nancy Strockbine, Claire Jenkins, Kaisar A. Talukder, Raymond Bercion, Konstantin Kuleshov, Renáta Kolínská, Julie E. Russell, Lidia Kaftyreva, Marie Accou-Demartin, Andreas Karas, Olivier Vandenberg, Alison E. Mather, Carl J. Mason, Andrew J. Page, Thandavarayan Ramamurthy, Chantal Bizet, Andrzej Gamian, Isabelle Carle, Amy Gassama Sow, Christiane Bouchier, Astrid Louise Wester, Monique Lejay-Collin, Marie-Christine Fonkoua, Simon Le Hello, Martin J. Blaser, Cecilia Jernberg, Corinne Ruckly, Audrey Mérens, Anne-Laure Page, Martin Aslett, Peter Roggentin, Angelika Fruth, Erick Denamur, Malabi Venkatesan, Hervé Bercovier, Ladaporn Bodhidatta, Chien-Shun Chiou, Dominique Clermont, Bianca Colonna, Svetlana Egorova, Gururaja P. Pazhani, Analia V. Ezernitchi, Ghislaine Guigon, Simon R. Harris, Hidemasa Izumiya, Agnieszka Korzeniowska-Kowal, Anna Lutyńska, Malika Gouali, Francine Grimont, Céline Langendorf, Monika Marejková, Lorea A.M. Peterson, Guillermo Perez-Perez, Antoinette Ngandjio, Alexander Podkolzin, Erika Souche, Mariia Makarova, German A. Shipulin, Changyun Ye, Helena Žemličková, Mária Herpay, Patrick A. D. Grimont, Julian Parkhill, Philippe Sansonetti, Kathryn E. Holt, Sylvain Brisse, Nicholas R. Thomson, and François-Xavier Weill

Subjects
Microbiology (medical), Immunology, Genetics, Cell Biology, Applied Microbiology and Biotechnology, Microbiology
Published
2016
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30. Erratum: High current density GaAs/Si rectifying heterojunction by defect free Epitaxial Lateral overgrowth on Tunnel Oxide from nano-seed

Author

Renard, Charles, Molière, Timothée, Cherkashin, Nikolay, Alvarez, José, Vincent, Laetitia, Jaffré, Alexandre, Hallais, Géraldine, Connolly, James Patrick, Mencaraglia, Denis, and Bouchier, Daniel

Subjects
Multidisciplinary, Erratum
Abstract

Interest in the heteroepitaxy of GaAs on Si has never failed in the last years due to the potential for monolithic integration of GaAs-based devices with Si integrated circuits. But in spite of this effort, devices fabricated from them still use homo-epitaxy only. Here we present an epitaxial technique based on the epitaxial lateral overgrowth of micrometer scale GaAs crystals on a thin SiO2 layer from nanoscale Si seeds. This method permits the integration of high quality and defect-free crystalline GaAs on Si substrate and provides active GaAs/Si heterojunctions with efficient carrier transport through the thin SiO2 layer. The nucleation from small width openings avoids the emission of misfit dislocations and the formation of antiphase domains. With this method, we have experimentally demonstrated for the first time a monolithically integrated GaAs/Si diode with high current densities of 10 kA.cm(-2) for a forward bias of 3.7 V. This epitaxial technique paves the way to hybrid III-V/Si devices that are free from lattice-matching restrictions, and where silicon not only behaves as a substrate but also as an active medium.

Published
2016
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31. Molecular characterization of Orf virus in goats in Gabon, Central Africa

Author

Eric M. Leroy, Labib Bakkali-Kassimi, Gael Darren Maganga, Anthony Relmy, Thierry Audrey Tsoumbou, Barthélémy Ngoubangoye, Stéphan Zientara, Nicolas Berthet, Christiane Bouchier, Nadine N'Dilimabaka, Institut National Supérieur d'Agronomie et de Biotechnologie (INSAB), Université des Sciences et Techniques de Masuku (USTM), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Centre International de Recherches Médicales de Franceville (CIRMF), Génomique (Plate-Forme) - Genomics Platform, Institut Pasteur [Paris] (IP), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Epidémiologie et Physiopathologie des Virus Oncogènes (EPVO (UMR_3569 / U-Pasteur_3)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut National Supérieur d’Agronomie et de Biotechnologies (INSAB), Institut Pasteur [Paris], Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre International de Recherches Médicales de Franceville, and Génomique (Plate-Forme)

Subjects
0301 basic medicine, 030106 microbiology, Buccal swab, Short Report, Sequence Homology, Biology, Polymerase Chain Reaction, DNA sequencing, law.invention, Evolution, Molecular, 03 medical and health sciences, law, Phylogenetics, Virology, Genetic variation, Ecthyma, Contagious, Animals, Cluster Analysis, Humans, Gabon, Gene, Phylogeny, Polymerase chain reaction, Sheep, High-throughput sequencing, Phylogenetic tree, Goats, Strain (biology), Genetic Variation, High-Throughput Nucleotide Sequencing, Orf virus, 3. Good health, PCR, 030104 developmental biology, Infectious Diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Abstract

International audience; Background: Orf or contagious ecthyma is a zoonotic viral infection with a potential serious health threat for the small ruminants industry as well as humans. It is currently emerging in new territories.Results: Eight suspected clinical cases of pustular dermatitis in goats occurred in the rural area of Tebe, in south-eastern Gabon, in January 2013. The orf virus (ORFV) was detected by high-throughput sequencing on sera, buccal swabs and scab pool samples. It was confirmed in six out of eight sick goats by using specific PCR targeting the major envelope protein (B2L) and the orf virus interferon resistance (VIR) genes. Phylogenetic analysis revealed that the Gabonese strain and South Korean strains evolved from a common ancestor, suggesting an Asian origin of the ORFV’ Gabonese strain.Conclusions: This study provides the molecular detection of the ORFV strain involved in the cases of pustular dermatitis in goats and highlights its circulation in Gabon

Published
2016
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32. Comments on the article: 'Enhancing the photovoltaic effect in the infrared region by germanium quantum dots inserted in the intrinsic region of a silicon p-i-n diode with nanostructure' by H. M. Tawancy (Journal of Materials Science DOI: 10.1007/s10853-011-5728-9)

Author

Daniel Bouchier and O. Aboelfotoh

Subjects
Materials science, Fabrication, Silicon, business.industry, Mechanical Engineering, Doping, chemistry.chemical_element, Germanium, Photovoltaic effect, law.invention, chemistry, Stack (abstract data type), Mechanics of Materials, Quantum dot, law, Solar cell, Optoelectronics, General Materials Science, business
Abstract

This comment letter report on a critical reading of an article published in Journal of Materials Science [1]. As stated in the acknowledgements, the silicon-based solar cells described in the article under question were contractually fabricated at the Institut d’Electronique Fondamentale, by using the technological facilities of the University Technology Center (CTU IEF-Minerve) of the University Paris Sud, France. The fabrication of prototype devices on request of external research institutions is part of the current assignments of this Center. The corresponding invoice was received from the author of Ref [1] in April, 2009. Using our previous know-how in epitaxial growth, a number of structures have been fabricated, consisting of a stack of Ge quantum dots, a Si layer and a highly doped Si layer, as described in Fig. 1 of ref. [1]. The solar cell devices based on these structures were completed with back and front contacts, as seen in Fig. 8 of Ref [1]. In addition, structural and optical routine characterizations were done in order to calibrate the areal quantum dot density and check the quality of the grown material, consisting in scanning electron microscopy imaging, and photoluminescence (PL) measurements made at room temperature. In collaboration with Prof. O. Aboelfotoh, we also checked the current voltage characteristics of the whole devices in the dark and under AM 1.5 standard illumination by using the dedicated facility of the Laboratoire de Genie Electrique de Paris, France. These devices and attached data were finally provided in 2010 to the King Fahd University of Petroleum & Minerals, Saudi Arabia. At this step, Dr H.M. Tawancy was of course free to act in his own way, like publishing results obtained from these devices. The corresponding article, entitled ‘‘Enhancing the photovoltaic effect in the infrared region by germanium

Published
2011
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33. Faceting mechanisms of Si nanowires and gold spreading

Author

Laetitia Vincent, Charles Renard, Frédéric Fossard, Daniel Bouchier, Vy Yam, Gilles Patriarche, C. Gardes, and R. Boukhicha

Subjects
Faceting, Crystallography, Materials science, Mechanics of Materials, Chemical physics, Mechanical Engineering, Nanowire, General Materials Science, Silicon nanowires, Threefold symmetry, Nanoclusters
Abstract

We report detailed structural analysis of 〈111〉 oriented silicon nanowires (NWs) grown by UHV–CVD using the VLS process with a gold catalyst. STEM-HAADF observations have revealed an unexpected inhomogeneous distribution of gold nanoclusters on the NW surface. Gold is mainly distributed on three sides among the six {112}-sidewalls and is anchored on upward {111} facets. This original observation brought us a new comprehension of the faceting mechanisms. The stability of the 〈111〉 growth direction needs the formation of facets on {112}-sidewalls with energetically favorable planes. We demonstrate that the initial formation of covered facets with a three-fold symmetry is driven by the formation of {111} Au/Si interfaces between the nucleated Si NW and the Au droplet.

Published
2011
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34. Tight glycaemic control is a key factor in wound healing enhancement strategies in an experimental diabetes mellitus model

Author

J. B. O’Sullivan, David Bouchier-Hayes, Richard Hanson, and F. Chan

Subjects
Blood Glucose, Male, medicine.medical_specialty, Skin wound, Glucose control, Diabetes Mellitus, Experimental, Rats, Sprague-Dawley, Transforming Growth Factor beta1, Hydroxyproline, chemistry.chemical_compound, Tensile Strength, Internal medicine, Diabetes mellitus, medicine, Animals, Experimental Diabetes Mellitus, Wound Healing, integumentary system, business.industry, General Medicine, Fibroblasts, medicine.disease, Immunohistochemistry, Body Fluids, Rats, Endocrinology, chemistry, Models, Animal, Diabetic wound healing, business, Wound healing, Transforming growth factor
Abstract

Diabetes mellitus is a leading cause of impaired wound healing. The aim of this study was to establish a glucose-controlled diabetic wound healing model. Sprague–Dawley rats were divided into three groups: Control group (C), Diabetic Non-glucose Controlled group (DNC) and Diabetic glucose Controlled group (DC). Glucose control was achieved using Insulman Rapid (average daily glucose level

Published
2010
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35. Comparison of arterial stiffness and microcirculatory changes following abdominal aortic aneurysm grafting

Author

D. H. O’ Donnell, E. G. Kavanagh, Michael A. Moloney, P. A. Grace, Patricia Fitzgerald, Rowan G. Casey, Seamus McHugh, and David Bouchier-Hayes

Subjects
Male, Applanation tonometry, medicine.medical_specialty, Manometry, Hemodynamics, Blood Pressure, Internal medicine, medicine.artery, Aneurysmal disease, Laser-Doppler Flowmetry, medicine, Humans, Aorta, Abdominal, cardiovascular diseases, Risk factor, Aged, Aorta, business.industry, Microcirculation, General Medicine, Laser Doppler velocimetry, medicine.disease, Elasticity, Abdominal aortic aneurysm, Surgery, Tissue Transplantation, cardiovascular system, Cardiology, Arterial stiffness, Female, business, Aortic Aneurysm, Abdominal
Abstract

Abdominal aortic aneurysm (AAA) surgery provides a unique opportunity to study the impact of arterial stiffness on central haemodynamics, reflected in augmentation index (AI). The aneurysmal aorta is significantly stiffer than undilated age-matched aorta. We investigated whether replacement of an aneurysmal aorta with a compliant graft would result in a decrease in AI, which would thus decrease myocardial workload parameters. Patients undergoing elective open or endovascular AAA repair were assessed with applanation tonometry and laser fluximetry pre-operatively, immediately and long-term post-operatively. Replacement of a small segment of abnormal conduit vessel resulted in improvements in AI, demonstrating that arterial stiffness can be surgically manipulated. These results reflect a decreased myocardial workload post-aortic grafting. This decrease in AI is important from a risk factor management perspective, and arterial stiffness should become a further recognised and screened for risk factor in patients with known aneurysmal disease.

Published
2010
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36. Electrical field stimulation promotes anastomotic healing in poorly perfused rat colon

Author

John B. Conneely, David Bouchier-Hayes, Rory Kennelly, and Desmond C. Winter

Subjects
Male, Vascular Endothelial Growth Factor A, medicine.medical_specialty, Pathology, Colon, Urology, Ischemia, Electric Stimulation Therapy, Anastomosis, Placebo, Rats, Sprague-Dawley, Hydroxyproline, chemistry.chemical_compound, Pressure, medicine, Animals, Wound Healing, Interleukin-6, business.industry, Anastomosis, Surgical, Angiography, Gastroenterology, medicine.disease, Rats, Perfusion, Vascular endothelial growth factor, chemistry, Sephadex, business, Ligation
Abstract

Introduction Hypoperfusion of the bowel is a risk factor for anastomotic failure. Electrical field stimulation has been shown to improve repair in ischemic tissue, but its influence in hypoperfused colon has not been investigated. The hypothesis of this experimental animal study was that electrical field stimulation improves anastomotic healing in ischemic bowel. Materials and methods Thirty rats were divided evenly into three groups: control, ischemia/placebo, and ischemia/test group. Ischemia was induced by ligation of the arterial supply to the proximal colon. The watershed area was identified and transected. Field stimulation was achieved by application of negatively charged diethylaminoethyl Sephadex beads in methylcellulose gel to the colonic epithelium prior to anastomosis. The placebo group had methylcellulose gel only applied and control animals had anastomosis only. Anastomotic strength was measured using anastomotic bursting pressure and hydroxyproline content. Systemic effect was investigated via interleukin-6 and vascular endothelial growth factor assay. Results The ischemia/electrical field stimulation (EFS) group had significantly increased bursting pressure and hydroxyproline content in comparison with the placebo group (P

Published
2010
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37. Anaplastic thyroid cancer, tumorigenesis and therapy

Author

James P. O'Neill, Michael Walsh, Derek G. Power, Claire Condron, and David Bouchier-Hayes

Subjects
Vascular Endothelial Growth Factor A, Oncology, medicine.medical_specialty, medicine.medical_treatment, Antineoplastic Agents, Malignancy, medicine.disease_cause, Cyclins, Internal medicine, medicine, Humans, Endocrine system, Thyroid Neoplasms, Anaplastic thyroid cancer, Anaplasia, beta Catenin, Chemotherapy, Radiotherapy, business.industry, Thyroid, General Medicine, Cadherins, medicine.disease, ErbB Receptors, Radiation therapy, medicine.anatomical_structure, Disease Progression, medicine.symptom, Carcinogenesis, business
Abstract

Anaplastic thyroid cancer (ATC) is a fatal endocrine malignancy. Current therapy fails to significantly improve survival. Recent insights into thyroid tumorigenesis, post-malignant dedifferentiation and mode of metastatic activity offer new therapeutic strategies. An extensive literature search of Medline and Pubmed was conducted to include all published reports on ATC. Secondary articles were identified from key paper reference listings. Significant progress, in the last 5 years, has been made outlining thyroid tumorigenesis and the progression to anaplasia. Continued identification and development of drug therapies is required to counter specific molecular targets responsible for the post-malignant dedifferentiation process and ultimately the fatal neoplastic phenotype.

Published
2009
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38. Taurine attenuates radiation-induced lung fibrosis in C57/Bl6 fibrosis prone mice

Author

M. Moriarty, Claire Condron, David Bouchier-Hayes, William B. Robb, and Thomas N. Walsh

Subjects
Pathology, medicine.medical_specialty, Taurine, Time Factors, Pulmonary Fibrosis, medicine.medical_treatment, Radiation induced, Transforming Growth Factor beta1, Mice, Hydroxyproline, chemistry.chemical_compound, Fibrosis, Pulmonary fibrosis, medicine, Animals, chemistry.chemical_classification, Analysis of Variance, Radiotherapy, business.industry, Lung fibrosis, General Medicine, Thorax, respiratory system, medicine.disease, respiratory tract diseases, Amino acid, Radiation therapy, Disease Models, Animal, chemistry, Female, business, Bronchoalveolar Lavage Fluid
Abstract

The amino acid taurine has an established role in attenuating lung fibrosis secondary to bleomycin-induced injury. This study evaluates taurine's effect on TGF-beta1 expression and the development of lung fibrosis after single-dose thoracic radiotherapy.Four groups of C57/Bl6 mice received 14 Gy thoracic radiation. Mice were treated with taurine or saline supplementation by gavage. After 10 days and 14 weeks of treatment, TGF-beta1 levels were measured in serum and bronchoalveolar lavage fluid (BALF). Lung collagen content was determined using hydroxyproline analysis.Ten days post radiotherapy, serum TGF-beta1 levels were significantly lower after gavage with taurine rather than saline (P = 0.033). BALF TGF-beta1 at 10 days was also significantly lower in mice treated with taurine (P = 0.031). Hydroxyproline content was also significantly lower at 14 weeks in mice treated with taurine (P = 0.020).This study presents novel findings of taurine's role in protecting from TGF-beta1-associated development of lung fibrosis after thoracic radiation.

Published
2009
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39. Neoplasms escape selective COX-2 inhibition in an animal model of breast cancer

Author

T. G. Neilan, M. Barry, Ronan A. Cahill, G. Roche-Nagle, David Bouchier-Hayes, Achim Treumann, and Judith H. Harmey

Subjects
Mice, Inbred BALB C, Cyclooxygenase 2 Inhibitors, business.industry, Lipoxygenase, Cancer, Breast Neoplasms, General Medicine, medicine.disease, Mice, Random Allocation, Animal model, Breast cancer, Lipoxygenase Inhibitors, Cyclooxygenase 2, Models, Animal, Immunology, Disease Progression, Cancer research, Animals, Medicine, Female, business, Antagonism
Abstract

Cyclo-oxygenase-2 (COX-2) is up-regulated in malignant tumours rendering it an attractive target for cancer therapeutics. However, whether long-term antagonism maintains its initial efficacy on established tumours is unclear.4T1 cells were injected into the mammary fat pad of BALB/c mice (n = 8). Once tumour deposits were established, animals were randomized into two equal groups to receive either a selective COX-2 inhibitor (SC-236) or a drug vehicle. Further animals similarly treated (n = 7) were studied in diuresis cages allowing urine capture and analysis by mass spectrometry to determine Prostaglandin F-1 levels (PGF-1). In addition, both wild-type receiving SC-236 and COX-2 knockout mice receiving either SC 236 or vehicle were subjected to the same studies to determine whether tumour-derived or host-derived (stromal) COX-2 was the critical element. Finally, BALB/c mice with 4T1 tumours (n = 7) were treated with a combination of COX-2 and lipoxygenase (LOX) inhibition to attenuate this escape phenomenon.While selective COX-2 inhibition initially retarded tumour growth, a rapid increase in tumour growth rate occurred later (day 9). This escape phenomenon correlated with an increase in urinary PGF-1 levels. An identical trend was also observed whether COX-2 knockout mice received SC-236 or not, suggesting that this effect is due to increased tumour-derived COX-2 production rather than recovery of host COX-2 functional capacity. Finally, dual inhibition of COX and LOX pathways attenuated this escape process.The anti-neoplastic effects of selective COX-2 inhibition may not be sustained as tumours demonstrate an escape capacity. However, this phenomenon maybe attenuated by a combination of COX/LOX inhibitors.

Published
2009
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40. Preconditioning and its clinical potential

Author

P. Magill, David Bouchier-Hayes, Thomas Brendan Murphy, and Kevin J. Mulhall

Subjects
medicine.medical_specialty, Time Factors, Ischaemia-reperfusion injury, business.industry, media_common.quotation_subject, Myocardial Reperfusion Injury, General Medicine, Insult, Anesthesia, Ischemic Preconditioning, Myocardial, Humans, Medicine, Cardiac Surgical Procedures, business, Intensive care medicine, media_common
Abstract

Preconditioning is emerging as a simple, safe and highly effective means of attenuating local and systemic effects of medical and surgical insult. Its enormous potential has not yet been harnessed and ongoing work will continue to bring it to the fore. This article covers the history, development and future clinical potential of preconditioning with particular regard to surgical insult.

Published
2009
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41. Sylvester o’halloran surgical scientific meeting

Author

N. Relihan, G. McGreal, M. Murray, E. W. McDermott, N. J. O’Higgins, M. J. Duffy, D. A. McNamara, J. Harmey, J. H. Wang, D. Donovan, T. N. Walsh, D. J. Bouchier-Hayes, E. Kay, J. D. Kelly, H. P. Weir, P. F. Keane, S. R. Johnston, K. E. Williamson, P. W. Hamilton, D. McManus, M. Morrin, P. V. Delaney, D. C. Winter, B. J. Harvey, J. P. Geibel, G. C. O’Sullivan, C. P. Delaney, R. Coffey, T. F. Gorey, J. M. Fitzpatrick, N. F. Fanning, W. Kirwan, T. Cotter, D. Bouchier-Hayes, H. P. Redmond, G. Pidgeon, F. Fennessy, C. Kelly, R. Flavin, A. M. Rasheed, A. Leahy, E. E. Lang, M. T. P. Caldwell, W. A. Tanner, P. D. Kiely, M. O’Reilly, S. Tierney, M. Barry, J. Drumm, P. A. Grace, C. M. Gallagher, D. C. Grant, P. Connell, M. K. Barry, O. Traynor, J. M. P. Hyland, M. J. O’Sullivan, D. Evoy, W. O. Kirwan, B. Cannon, L. Kenny-Walshe, M. J. Whelton, H. O’Grady, S. O’Neill, J. M. Hyland, S. H. Teh, S. O’Ceallaigh, M. K. O’Donohoe, F. B. Keane, G. C. O’Toole, J. Calleary, L. Basso, S. B. Amjad, Z. Khan, L. McMullin, W. P. Joyce, P. J. Balfe, M. T. Caldwell, S. Teahan, K. Al-Brekeit, A. Rasheed, A. Cullen, C. O’Keane, J. MacFarlane, M. Walsh, T. McGloughlin, P. Grace, D. Colgan, P. Madhavan, S. Sultan, M. P. Colgan, D. Moore, G. Shanik, N. McEniff, M. Molloy, E. Eguare, C. Fiuza, P. Burke, R. Maher, M. Creamer, C. J. Cronin, H. H. Sigurdsso, W. Kim, G. Linklater, K. S. Cross, W. G. Simpson, J. A. M. Shaw, D. W. M. Pearson, P. Fitzgerald, P. Quinn, C. M. Brady, S. M. A. Shah, M. Ehtisham, M. S. Khan, H. D. Flood, M. Loubani, K. Sweeney, B. Lenehan, V. Lynch, A. Joy, D. Reidy, K. Mahalingam, W. Cashman, E. D. Mulligan, T. Purcell, B. Dunne, M. Griffin, N. Noonan, D. Hollywood, N. Keeling, J. V. Reynolds, T. P. J. Hennessy, D. O’Halloran, P. Neary, D. Hamilton, N. Haider, N. Aherne, R. G. K. Watson, D. Walsh, M. Murphy, M. Joyce, S. Johnston, O. Clinton, H. F. Given, A. Brannigan, M. O’Donohoe, J. Donohoe, T. Corrigan, M. Bresnihan, T. M. Feeley, M. P. McMonagle, D. Quinlan, D. Kelly, P. K. Hegarty, B. Tan, C. Cronin, M. P. Brady, M. Zeeshan, D. J. McAvinchey, C. Mooney, D. Coyle, G. Khayyat, E. Masterson, T. Thambi-Pillai, K. Farah, M. B. Codd, G. G. Tsiotos, C. D. Johnson, M. G. Sarr, M. R. Kell, M. Lynch, D. Ryan, A. O’Donovan, M. Cassidy, M. Doyle, G. Fulton, P. R. O’Connell, R. Kingston, M. Dillon, E. McDermott, N. O’Higgins, R. G. O’Sullivan, and J. A. O’Donnell

Subjects
business.industry, Foundation (engineering), Medicine, Library science, General Medicine, business
Published
1998
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42. National scientific medical meeting 1997 abstracts

Author

H. J. Willison, A. J. Lastovica, M. M. Prendergast, A. P. Moran, C. Walsh, I. Flitcroft, P. Eustace, C. McMahon, J. Smith, O. P. Smith, G. Lakshmandass, M. R. H. Taylor, C. V. Holland, D. Cox, B. Good, G. M. Kearns, P. Gaffney, K. Shark, M. Frauenshuh, W. Ortmann, R. Messner, R. King, S. Rich, T. Behrens, N. Mahmud, A. Molloy, J. McPartlin, J. M. Scott, D. G. Weir, K. M. Walsh, D. Thorburn, P. Mills, A. J. Morris, T. Good, S. Cameron, E. A. B. McCruden, M. W. Bennett, J. O’Connell, C. Brady, D. Roche, J. K. Collins, F. Shanahan, G. C. O’Sullivant, M. Henry, S. Koston, K. McMahon, W. MacNee, M. X. FitzGerald, C. M. O’Connor, D. McGonagle, W. Gibbon, P. O’Connor, P. Emery, M. Murphy, R. Watson, E. Casey, E. Naidu, L. Barnes, S. McCann, E. Sweeney, E. J. Barrett, H. Graham, R. T. Cunningham, C. F. Johnston, W. J. Curry, K. D. Buchanan, C. H. Courtney, A. S. McAllister, D. R. McCance, D. R. Hadden, P. M. Bell, H. Leslie, B. Sheridan, A. B. Atkinson, M. T. Kilbane, D. F. Smith, M. J. Murray, S. G. Shering, E. W. M. McDermott, N. J. O’Higgins, P. P. A. Smyth, J. McEneny, E. R. Trimble, I. S. Young, P. Sharpe, C. Mercer, D. McMaster, A. E. Evans, J. Cundick, O. Hasselwander, J. McGeough, D. Savage, A. P. Maxwell, F. Kee, C. J. Larkin, R. G. P. Watson, C. Johnston, J. E. S. Ardill, D. A. McNamara, T. N. Walsh, D. J. Bouchier-Hayes, C. Madden, C. Timon, N. Gardiner, M. Lawler, J. O’Riordan, C. Duggan, S. R. McCann, H. Gowing, E. Braakman, C. Byrne, A. C. M. Martens, A. Hagenbeek, N. Kinsella, S. Cusack, H. Baker, B. White, K. Molloy, A. Wogan, S. McElwaine, D. Hollywood, C. Mcmahon, C. Merry, M. Ryan, O. Smith, F. M. Mulcahy, C. Murphy, J. Briones, P. Lavin, M. McCaffrey, P. Gillen, L. Thompson, M. Lalloz, M. Layton, C. Corish, N. P. Kennedy, P. Flood, S. Mulligan, E. McNamara, P. M. Mathias, E. Ball, D. Duiculescu, P. Calistru, N. O’Gorman, M. Abuzakouk, C. Feighery, M. Brannigan, S. Pender, F. Keeling, J. Varghese, M. Lee, M. Colreavy, R. Gaffney, S. Hone, M. Herzig, M. Walsh, C. Dolan, D. Donovan, J. Harmey, A. Haverty, J. H. Wang, J. H. Harmey, H. P. Redmond, G. McGreal, M. J. Moriarty, A. Shortt, E. Kay, G. Pidgeon, P. Dunne, H. Lambkin, J. M. Russell, A. J. O’Neill, B. M. Dunne, M. O’Donovan, E. F. Gaffney, J. E. Gillan, T. G. Cotter, J. Horan, D. Jones, S. K. Biswas, E. C. Mulkerrin, H. Brady, J. O’Donnell, J. Neary, E. Healy, A. Watson, B. Keogh, C. Cassidy, S. Ward, E. Stokes, F. Keoghan, A. Barrett, P. O’Connell, N. Ryall, P. A. O’Connell, A. Jenkinson, T. O’Brien, P. G. O’Connell, R. Harrison, T. Barrett, D. M. D. Bailey, A. Butler, D. E. Barton, G. Daly, M. Gill, S. Heron, Z. Hawi, M. Fitzgerald, L. Mynett-Johnson, D. Shiels, K. Kendler, P. McKeon, R. Straub, D. Walsh, F. Ryan, D. McCabe, R. Murphy, R. Segurado, T. Mulcahy, B. Larson, C. Comerford, R. O’Connell, E. O’Mahony, J. Donnelly, F. Minahan, D. O’Neill, Z. Farrell, C. Glynn, E. Mulkerrin, S. E. Lennox, A. Murphy, I. M. Rea, H. McNulty, C. McMeel, H. McEvoy, R. Freaney, M. J. McKenna, M. Crowe, D. Keating, G. Norman, S. Widda, L. Viani, null Galvin, C. M. Nolan, O. Hardiman, F. Brett, O. Droogan, P. Gallagher, M. Harmey, M. King, J. Murphy, R. Perryrnan, S. Sukumaran, J. Walsh, M. A. Farrell, G. Hughes, C. Cunningham, J. B. Walsh, D. Coakley, M. Hurson, P. McMonagle, S. O’Sullivan, P. Dodd, J. Redmond, R. Browne, S. Keating, J. O’Connor, B. P. Cassidy, R. Smyth, N. P. Sheppard, R. Cullivan, J. Crown, N. Walsh, A. Denihan, I. Bruce, A. Radic, B. A. Lawlor, P. K. Bridges, M. O’Doherty, A. Farrington, B. Farragher, S. Fahy, R. Kelly, T. Carey, J. Owens, O. Gallagher, D. Sloan, C. McDonough, P. Casey, A. Horgan, A. Elneihum, C. O’Neill, T. McMonagle, J. Quinn, D. Meagher, P. Murphy, A. Kinsella, J. Mullaney, J. L. Waddington, S. Rooney, L. Bamford, J. J. O’Connor, R. Franklin, K. O’Brien, G. Fitzpatrick, J. G. Laffey, J. F. Boylan, J. Laffey, M. Coleman, J. Boylan, A. J. McShane, J. P. R. Loughrey, J. Gardiner, J. McGinley, I. Leonard, M. Carey, P. Neligan, J. O’Rourke, A. Cunningham, F. Fennessy, C. Kelly, D. Bouchier-Hayes, J. Kellett, D. Murphy, J. Regan, D. O’Keeffe, A. Mahmud, L. Hemeryck, J. Feely, M. Hall, I. B. A. Menown, T. P. Mathew, G. S. Nesbitt, M. Syme, A. A. J. Adgey, F. Turtle, J. Allen, J. Anderson, R. O’Hanlon, M. B. Codd, S. Walkin, H. A. McCann, D. D. Sugrue, A. M. Rasheed, G. Chen, A. Leahy, S. Jina, I. McDowell, Q. Wo, M. N. Shuhaibar, E. McGovern, G. Manoharan, R. Kirkpatrick, N. P. S. Campbell, C. McCarthy, Y. Wen, S. Killalea, C. J. Fahy, A. Griffith, A. Fraser, T. Ryan, M. Browne, J. Fenton, J. Hughes, C. I. Timon, A. Curran, D. Smyth, J. P. Hughes, P. Lee, A. Kelly, N. Shine, A. Blayney, D. P. McShane, J. Hussey, M. Howlett, A. Langton, A. McEvoy, J. Slevin, C. Fitzpatrick, M. J. Turner, F. Enright, N. Goggin, C. Costigan, D. Duff, P. Osizlok, F. Wood, R. B. Fitzsimons, N. Flanagan, E. Molloy, E. Griffin, P. F. Deasy, M. Sheridan, M. J. White, R. Moore, A. Gray, J. Hill, J. F. T. Glasgow, B. Middleton, D. Slattery, V. Donoghue, A. McMahon, A. McCarthy, P. Oslislok, I. Keogh, K. J. Russell, M. X. Fitzgerald, P. V. Kavanagh, S. M. McNamara, M. Barry, J. E. O’Brien, P. McCormick, C. Molony, R. M. Doyle, P. R. O’Connell, L. C. Dowey, H. McGlynn, D. I. Thurnham, S. J. Elborn, L. Flynn, J. Carton, B. Byrne, C. O’Farrelly, P. Kelehan, C. O’Herlihy, A. M. O’Hara, A. Orren, B. A. Fernie, S. Clarke, G. Courtney, C. de Gascun, M. Byrne, E. Moylett, H. Murphy, K. Butler, C. Nourse, H. Thaker, C. Barry, J. Russell, G. Sheehan, B. Boyle, R. Hone, B. Conboy, C. Butler, D. Moris, M. Cormican, J. Flynn, O. McCormack, N. Corbally, A. Murray, S. Kirrane, C. O’Keane, S. M. Lynch, B. Cryan, D. Whyte, D. Morris, G. Corbett-Feeney, T. Mackle, J. Perkins, C. Saidlear, A. Young, M. Wrigley, J. Clifford, O. Tighe, D. T. Croke, J. Drago, D. R. Sibley, M. Carvalho, M. Hennessy, M. Kelly, C. Hughes, M. Hanlon, K. Sabra, T. Keane, D. Egan, C. Maerry, S. C. Sharma, D. Williams, N. G. Mahon, G. M. Sayers, and Z. Johnson

Subjects
Medical education, business.industry, Medicine, General Medicine, business
Published
1998
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43. Sylvester o’halloran surgical scientific meeting

Author

G. J. Fulton, M. G. Davies, P. O’Hagen, A. Rasheed, C. Kelly, E. Kay, S. Fitzgerald, D. Bouchier-Hayes, A. Leahy, F. Fennessy, P. Fitzgerald, K. Khosraviani, H. P. Weir, K. Williamson, R. Wilson, R. J. Moorehead, B. J. Rowlands, D. Morrissey, J. O’Connell, D. Lynch, C. O’Sullivan, F. Shanahan, J. K. Collins, J. L. Kelly, C. C. Soberg, A. Lyons, J. A. Mannick, J. A. Lederer, C. Chen, D. J. Bouchier-Hayes, H. Fitzsimons, D. M. O’Hanlon, C. Curran, M. Canney, S. Morris, O. Clinton, H. F. Given, E. Coveney, H. K. Lyerly, F. L. Murphy, C. J. Kelly, D. H. Osborne, P. Kelly, D. S. O’Riordan, P. G. Horgan, F. B. V. Keane, W. A. Tanner, P. Kilmartin, C. P. Delaney, S. M. Johnston, J. M. Fitzpatrick, T. F. Gorey, J. Mehigan, M. G. O/rsRiordan, N. Shines, A. Hill, C. O. McDonnell, F. Murphy, S. M. Javadpour, Y. Alhadi, R. Waldron, R. G. Watson, A. Tarrant, T. K. Neelamekam, J. Mathias, J. Geoghegan, T. Boyle, O. Traynor, S. Hayes, B. O’Donovan, N. Ajmal, J. McCann, N. T. Corrigan, M. G. O’Riordan, P. Ross, M. O’Donohoe, M. Bresnihan, T. M. Feeley, C. Fiuza-Castineira, D. Coleman, H. Fisher, A. Butt, E. Ghumman, P. Grace, P. Burke, S. A. Martin, M. K. Fox-Talbot, P. A. Lipsett, K. D. Lillemoe, H. A. Pitt, D. A. O’Keeffe, A. D. K. Hill, K. Sheahan, F. Ryan, D. Barton, R. Fitzgerald, E. W. McDermott, N. J. O’Higgins, E. Kavanagh, P. Kiely, D. O’Driscoll, M. Ramesh, W. O. Kirwan, D. C. Winter, K. Nally, J. O’Callaghan, J. B. Matthews, B. J. Harvey, G. C. O’Sullivan, L. S. Young, M. C. Regan, P. Sweeney, D. M. Bouchier-Hayes, R. Dardis, P. Broe, M. G. O’Brien, P. Neary, P. Ridgeway, C. Condron, J. H. Wang, H. P. Redmond, D. R. M. Redfern, R. K. S. Strachan, J. M. Hollingdale, P. A. Grace, A. Acheson, A. Graham, C. Weir, B. Lee, C. O’Donnell, D. Buckley, J. A. O’Donnell, E. Purcell, M. O’Donoghue, S. Sultan, M. Colgan, M. Molloy, D. Moore, G. Shanik, P. T. McCollum, Z. Raza, S. Naidu, P. A. Stonebridge, M. P. Colgan, D. J. Moore, D. G. Shanik, J. Dowdall, C. Williams, S. G. Shering, G. Duffy, R. Greengrass, D. Iglehart, G. Little, H. Kim Lyerly, M. Fynes, A. Cahill, C. O’Herlihy, P. R. O’Connell, I. Ahmad, M. Etisham, J. Drumm, H. Flood, K. Mulhall, K. Murray, S. O’Rian, N. Garvey, J. Johnston, G. T. McGreal, M. P. Brady, M. M. Duffy, M. Regan, M. G. Harrington, M. Javadpour, C. McDonnell, E. Eguare, M. C. Barry, G. C. O’Toole, N. O’Higgins, E. McDermott, C. M. Brady, S. A. Sultan, M. K. O’Donoghue, M. P. Molloy, G. D. Shanik, R. J. Holdsworth, M. Fehily, C. Doran, F. Keane, J. F. Rothwell, M. J. Staunton, L. O’Mahony, E. F. Gaffney, K. Mealy, T. P. J. Hennessy, and J. Geibel

Subjects
business.industry, Art history, Medicine, General Medicine, business
Published
1998
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44. Waterford surgical October club and surgical section, Royal Academy of Medicine Joint Surgical Symposium

Author

F. J. Shannon, T. J. Boyle, D. C. Grant, O. J. Clyne, J. M. P. Hyland, E. D. Mulligan, T. Purcell, P. Lawlor, J. V. Reynolds, P. J. Byrne, T. P. J. Hennessy, M. Duff, A. D. K. Hill, S. G. Shering, E. W. McDermott, N. J. O’Higgins, M. Harte, J. Corrigan, S. Khurana, F. Manning, B. Kierce, M. Corbally, D. A. McNamara, E. Dimitriadis, E. Kay, H. P. Redmond, J. Harmey, D. J. Bouchier-Hayes, G. Chen, C. Kelly, H. Chen, A. Leahy, J. Bouchier-Hayes, D. C. Winter, K. Nally, J. O’Callaghan, B. J. Harvey, F. Shanahan, G. C. O’Sullivan, H. Wang, Q. D. Wu, C. Condron, and D. Bouchier-Hayes

Subjects
medicine.medical_specialty, business.industry, General surgery, Medicine, Joint (building), General Medicine, Club, business, Surgical section, Surgery
Published
1998
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45. Irish society of gastroenterology

Author

G. Clarke, E. Ryan, J. C. O’Keane, J. Crowe, P. McMathuna, D. Moriarty, R. Ettarh, K. Sheahan, J. Hyland, D. P. O’Donoghue, A. W. Baird, G. Gormley, J. C. O. Keane, P. MacMathuna, J. H. Wang, Q. D. Wu, H. P. Redmond, C. Condron, D. Bouchier-Hayes, K. Nally, F. Newton, J. O’Connell, G. C. O’Sullivan, J. Morgan, J. K. Collins, F. Shanahan, C. Goode, D. C. Winter, C. T. Taylor, M. M. Skelly, B. J. Harvey, J. C. Varghese, M. A. Farrell, F. P. McGrath, F. E. Murray, H. Osborne, M. J. Lee, A. Sullivan, A. E. Ryan, A. N. Donovan, P. A. McCormick, B. Kenny, S. Somers, A. Bohan, R. G. Gibney, M. Marcaccio, D. E. Malone, M. Doyle, C. P. Delaney, T. F. Gorey, G. P. McEntee, A. Clarke, R. Stuart, J. Kelly, M. D. Kiely, M. O’Sullivan, E. Lovett, N. Mahmud, D. Kelleher, C. A. O’Morain, C. J. Larkin, R. G. P. Watson, J. M. Sloan, J. E. S. Ardill, C. F. Johnston, K. D. Buchanan, A. Heaney, J. S. A. Collins, G. R. P. Watson, R. M. Kalin, T. C. K. Tham, R. J. McFarland, K. B. Bamford, T. Ó Cróinín, M. Clyne, B. Drumm, M. Rowland, D. Kumar, P. O’Connor, L. E. Daly, D. L. O’Toole, A. Long, A. M. Murphy, L. O’Neill, D. G. Weir, A. M. Hopkins, P. Moynagh, C. Brennan, J. Harmey, P. P. Stapleton, A. M. Rasheed, G. Chen, C. Kelly, D. J. Bouchier-Hayes, A. Leahy, M. Gallagher, A. Grace, Y. Xin, M. Leader, E. Kay, A. Whelan, U. Pattison, R. Willoughby, E. Wallace, D. Weir, C. Feighery, M. W. Bennett, C. Brady, D. Roche, A. Molloy, J. McPartlin, J. M. Scott, A. G. Acheson, J. Lee, K. Khosraviani, S. T. Irwin, J. McDaid, J. R. Docherty, A. O’Grady, M. Mabruk, C. Johnston, W. Curry, J. Ardill, R. Cunningham, N. I. McDougall, P. V. Coyle, M. E. Callender, A. M. Ouinn, R. Warner, F. M. Stevens, P. I. S. Chakravarthi, M. Kearns, M. Bourke, A. Hassan, J. McWeeney, C. F. McCarthy, M. Casey, J. O’Donoghue, A. M. Eustace-Ryan, P. O’Regan, L. Feighery, J. Jackson, N. Cronin, K. Quane, E. D. Mulligan, T. Purcell, B. Dunne, M. Griffin, N. Noonan, D. Hollywood, N. Keeling, J. V. Reynolds, T. P. J. Hennessy, M. O’Sulhvan, I. Harman, N. P. Breslin, N. Clayton, S. Hogan, B. Donovan, D. Hayes, M. Kiely, C. A. Goulding, S. S. Albloushi, J. O’Connor, M. G. Courtney, D. Royston, A. G. Shattock, A. Stack, M. Carmody, S. Barrett, A. Hennigan, L. Young, C. J. Shields, C. O’Keane, J. M. Fitzpatrick, M. M. Doyle, R. B. Stephens, P. A. Daly, G. M. Briggs, D. McCrory, S. O’Neill, H. O’Grady, D. C. Grant, K. Barry, O. Traynor, J. M. P. Hyland, G. C. O’Toole, M. K. Barry, S. D. Johnston, C. M. Ritchie, T. J. Robinson, J. M. Kirby, E. M. Mackle, N. Haider, N. Aherne, F. McNichol, D. Hamilton, P. Neary, S. Hegarty, J. O. Connor, R. G. K. Watson, D. Drudy, A. Alwan, L. Fenelon, C. O’Farrelly, B. Byrne, L. Madrigal, J. Carton, C. Collins, D. O’Donoghue, N. Gannon, A. Hickey, C. A. O’Boyle, R. Byrne, S. Albloushi, and F. Murray

Subjects
medicine.medical_specialty, Irish, business.industry, Ophthalmology, language, Library science, Medicine, General Medicine, business, language.human_language
Published
1998
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46. Recombinant bactericidal permeability increasing protein (rBPI21) inhibits surgery-induced tumour growth in a murine model of metastatic disease

Author

David Bouchier-Hayes, Henry Paul Redmond, G. T. O’Donoghue, Judith H. Harmey, R. Dedrick, and Graham P. Pidgeon

Subjects
Vascular Endothelial Growth Factor A, medicine.medical_specialty, Pathology, Lung Neoplasms, medicine.medical_treatment, Cell Culture Techniques, Apoptosis, Enzyme-Linked Immunosorbent Assay, Tissue Adhesions, Adenocarcinoma, law.invention, Mice, Random Allocation, Bacterial Proteins, law, Laparotomy, medicine, Animals, Neoplasm Metastasis, Mitosis, Cell Proliferation, Inflammation, Mice, Inbred BALB C, Lung, biology, Cell growth, business.industry, Membrane Proteins, Blood Proteins, General Medicine, Bactericidal/permeability-increasing protein, Recombinant Proteins, Blockade, Surgery, Endotoxins, medicine.anatomical_structure, Recombinant DNA, biology.protein, business, Antimicrobial Cationic Peptides
Abstract

Endotoxin (LPS), a cell wall constituent of gram-negative bacteria, is a potent inflammatory stimulus. We demonstrated that laparotomy increases primary tumour growth and experimental lung metastases, implicating endotoxin as a causative factor. We hypothesised that the anti-endotoxin agent, rBPI(21) would block surgery-induced tumour growth.Mammary adenocarcinoma cells were injected into female BALB/c mice to establish lung metastases. Mice were randomised into three groups receiving anaesthesia, laparotomy or laparotomy and rBPI(21) treatment on day 14. Animals were killed on day 19, lungs harvested and blood obtained. Number and size of lung metastases were recorded. Apoptosis, mitosis and microvessel density within metastases were assessed and VEGF measured.Laparotomy increased metastatic growth, decreased tumour cell apoptosis, increased tumour cell proliferation, increased microvessel density and circulating VEGF. LPS blockade by rBPI(21) attenuated this increased growth and decreased proliferation, increased apoptosis, decreased micro-vessel density and circulating VEGF. This suggests that rBPI(21), has clinical potential in attenuating surgery enhanced tumour growth, especially in patients with a history of cancer undergoing laparotomy.

Published
2008
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47. Preoperative selection of symptomatic breast cancer patients appropriate for lymphatic mapping and sentinel node biopsy

Author

R. G. K. Watson, David Bouchier-Hayes, Mary Barry, G. Roche-Nagle, R. Landers, David J Walsh, and Ronan A. Cahill

Subjects
Adult, Oncology, medicine.medical_specialty, Breast Neoplasms, Breast cancer, Internal medicine, Biopsy, medicine, Humans, Pathological, Breast ultrasound, Aged, Ultrasonography, Aged, 80 and over, medicine.diagnostic_test, Sentinel Lymph Node Biopsy, business.industry, Patient Selection, General Medicine, Middle Aged, Sentinel node, medicine.disease, Lymphatic disease, Axilla, medicine.anatomical_structure, Lymphatic system, Lymphatic Metastasis, Female, Radiology, business
Abstract

This study examines whether preoperative ultrasound-assessed tumour diameter and diagnostic core biopsy-determined grade can be used to select those most likely to benefit from SLNB (i.e. those that are “node negative”) before their definitive operation. Breast ultrasound (US) and a simultaneous core biopsy was performed in all patients at their initial presentation, and their estimates of tumor size and grade compared with the final pathological specimen (FPS). Of the T1 group 47% had lymphatic metastases as did 49% of those with grade I or II cancers. By combining these measures, however, subgroups of patients with lower rates of nodal metastases were identified (32% of patients with T1, non-grade III disease had lymphatic disease while only 15% of those with T

Published
2007
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48. Photoselective vaporization of the prostate – towards a new standard

Author

D M Bouchier-Hayes

Subjects
Cancer Research, medicine.medical_specialty, business.industry, Urology, medicine.medical_treatment, urologic and male genital diseases, law.invention, Surgery, medicine.anatomical_structure, Oncology, Randomized controlled trial, Quality of life, Blood loss, law, Prostate, medicine, Photoselective vaporization, Adverse effect, business, Sexual function, Transurethral resection of the prostate
Abstract

Many technologies have been mooted as equal to transurethral resection of the prostate (TURP) without gaining widespread acceptance owing to lack of randomized trials. The GreenLight laser system (American Medical Systems, Minnetonka, MN) gives an 80-W laser ablation system for photovaporization of the prostate (PVP) and here is compared to TURP in such a trial. One hundred and twenty patients are randomized to undergo TURP or PVP after evaluation, which is repeated at 1, 3, 6 and 12 months. Irrigation use, length of catherization time (LOC), length of hospital stay (LOS), blood loss, cost and operative time are also assessed. To date 87 patients are evaluable. In summary, both groups showed a significant increase in maximal flow rate (Qmax) from baseline (P

Published
2007
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49. No evidence for viral sequences in five lepidic adenocarcinomas (former 'BAC') by a high-throughput sequencing approach

Author

Nicolas Berthet, Nicolas Dorvault, Christiane Bouchier, Lionel Frangeul, Jean-Charles Soria, Elisabeth Brambilla, Antoine Gessain, Philippe Girard, Elie Fadel, Ken A. Olaussen, Pierre Validire, Epidémiologie et Physiopathologie des Virus Oncogènes (EPVO (UMR_3569 / U-Pasteur_3)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Virus et Interférence ARN - Viruses and RNA Interference, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Biomarqueurs prédictifs et nouvelles stratégies moléculaires en thérapeutique anticancéreuse (U981), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Michallon, Institut Mutualiste de Montsouris (IMM), Hypertension arterielle pulmonaire physiopathologie et innovation thérapeutique, Centre Chirurgical Marie Lannelongue (CCML)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris-Sud - Paris 11 (UP11), Génomique (Plate-Forme) - Genomics Platform, Institut Pasteur [Paris] (IP), Institut Pasteur [Paris]-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre chirurgical Marie Lannelongue, and Institut Pasteur [Paris]

Subjects
Male, Lung Neoplasms, MESH: Adenocarcinoma, Bronchiolo-Alveolar, MESH: Animals, MESH: High-Throughput Nucleotide Sequencing, Lung, MESH: Aged, Medicine(all), MESH: Middle Aged, High-Throughput Nucleotide Sequencing, Merkel Cell Carcinoma, General Medicine, Jaagsiekte sheep retrovirus, Middle Aged, respiratory system, MESH: Pulmonary Adenomatosis, Ovine, 3. Good health, MESH: Jaagsiekte sheep retrovirus, Pulmonary Adenomatosis, Ovine, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Adenocarcinoma, Infectious etiology, Female, MESH: Endogenous Retroviruses, Research Article, Oncogenic Virus, MESH: Sheep, Computational biology, Biology, General Biochemistry, Genetics and Molecular Biology, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], medicine, Carcinoma, Animals, Humans, MESH: Lung, Lung cancer, Aged, MESH: Humans, Sheep, Biochemistry, Genetics and Molecular Biology(all), Taxonomic Assignment, MESH: Adenocarcinoma, Endogenous Retroviruses, Lung Adenocarcinoma, Primary Effusion Lymphoma, Adenocarcinoma, Bronchiolo-Alveolar, medicine.disease, biology.organism_classification, Virology, MESH: Male, MESH: Lung Neoplasms, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], MESH: Female
Abstract

International audience; BackgroundThe hypothesis of an infectious etiology of the formerly named bronchiolo-alveolar carcinoma (BAC) has raised controversy. We investigated tumor lung tissues from five patients with former BAC histology using high-throughput sequencing technologies to discover potential viruses present in this type of lung cancer. Around 180 million single reads of 100 bases were generated for each BAC sample.ResultsNone of the reads showed a significant similarity for Jaagsiekte sheep retrovirus (JSRV) and no other viruses were found except for endogenous retroviruses.ConclusionsIn conclusion, we have demonstrated the absence of JSRV and other known human viruses in five samples of well-characterized lepidic adenocarcinoma.BackgroundThe bronchiolar-alveolar cancer (BAC) in its past definition (WHO classification 1999) is a rare form of lung adenocarcinoma (ADC). The international WHO 2015 classification recommends distinguishing adenocarcinoma in situ (AIS, formerly non-mucinous BAC) from invasive mucinous adenocarcinoma (IMA, formerly mucinous BAC) and non-mucinous lepidic predominant invasive adenocarcinoma of the lungs [1]. In many such patients, the tumor progression respects the pulmonary architecture and develops mainly in the terminal respiratory unit (lepidic growth).

Published
2015
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50. A highly stable prefusion RSV F vaccine derived from structural analysis of the fusion mechanism

Author

Lies Bogaert, Myra N. Widjojoatmodjo, Roland Zahn, Polina Furmanova-Hollenstein, Jason S. McLellan, Daphné Truan, Ilona J. M. Bisschop, Anders Krarup, Pascale Bouchier, Johannes P. M. Langedijk, and Hanneke Schuitemaker

Subjects
Protein Conformation, Viral protein, viruses, Blotting, Western, General Physics and Astronomy, Enzyme-Linked Immunosorbent Assay, Respiratory Syncytial Virus Infections, Biology, Crystallography, X-Ray, medicine.disease_cause, Article, General Biochemistry, Genetics and Molecular Biology, Virus, Mice, Protein structure, Antigen, Respiratory Syncytial Virus Vaccines, medicine, Animals, Sigmodontinae, Antigens, Viral, Mutation, Multidisciplinary, General Chemistry, Antibodies, Neutralizing, Fusion protein, Virology, Respiratory Syncytial Viruses, Microscopy, Electron, biology.protein, Electrophoresis, Polyacrylamide Gel, Antibody, Crystallization, Viral Fusion Proteins
Abstract

Respiratory syncytial virus (RSV) causes acute lower respiratory tract infections and is the leading cause of infant hospitalizations. Recently, a promising vaccine antigen based on the RSV fusion protein (RSV F) stabilized in the native prefusion conformation has been described. Here we report alternative strategies to arrest RSV F in the prefusion conformation based on the prevention of hinge movements in the first refolding region and the elimination of proteolytic exposure of the fusion peptide. A limited number of unique mutations are identified that stabilize the prefusion conformation of RSV F and dramatically increase expression levels. This highly stable prefusion RSV F elicits neutralizing antibodies in cotton rats and induces complete protection against viral challenge. Moreover, the structural and biochemical analysis of the prefusion variants suggests a function for p27, the excised segment that precedes the fusion peptide in the polypeptide chain., Respiratory syncytial virus (RSV) is a highly contagious childhood pathogen of the respiratory tract for which no vaccine is currently available. Here the authors present a strategy to stabilize the RSV F protein in a prefusion conformation that can elicit a strong protective immune response in animal models.

Published
2015
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