1. Gastroprotective action of Cochinchina momordica seed extract is mediated by activation of CGRP and inhibition of cPLA(2)/5-LOX pathway.
- Author
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Kang JM, Kim N, Kim B, Kim JH, Lee BY, Park JH, Lee MK, Lee HS, Jang IJ, Kim JS, Jung HC, and Song IS
- Subjects
- Animals, Calcitonin Gene-Related Peptide blood, Calcitonin Gene-Related Peptide genetics, Cyclooxygenase 2 metabolism, Cytoprotection, Dinoprostone metabolism, Disease Models, Animal, Down-Regulation, Ethanol, Gastric Mucosa enzymology, Gastric Mucosa pathology, Hexosamines metabolism, Inflammation Mediators metabolism, Interleukin-1beta metabolism, Leukotriene B4 metabolism, Male, Mucin 5AC metabolism, Mucin-6 metabolism, Nitric Oxide metabolism, Nitric Oxide Synthase antagonists & inhibitors, Nitric Oxide Synthase metabolism, Nitric Oxide Synthase Type I, Peroxidase metabolism, RNA, Messenger metabolism, Rats, Rats, Sprague-Dawley, Restraint, Physical, Seeds, Sensory Receptor Cells drug effects, Sensory Receptor Cells metabolism, Stomach Ulcer enzymology, Stomach Ulcer etiology, Stomach Ulcer pathology, Time Factors, Tumor Necrosis Factor-alpha metabolism, Arachidonate 5-Lipoxygenase metabolism, Calcitonin Gene-Related Peptide metabolism, Gastric Mucosa drug effects, Gastrointestinal Agents pharmacology, Group IV Phospholipases A2 metabolism, Momordica, Plant Extracts pharmacology, Signal Transduction drug effects, Stomach Ulcer prevention & control
- Abstract
Cochinchina momordica seed extract (SKMS10), which is composed of the major compounds momordica saponins, has been evaluated for its gastroprotective effects in rat models of acute gastric mucosal damage. Ethanol and water immersion restraint stress (WRS) induced gastric damage, including hemorrhages and edema, was significantly attenuated by pretreatment with SK-MS10. In addition, SK-MS10 reduced increases of mucosal myeloperoxidase (MPO), IL-1β, and TNFα levels and the expression of cPLA(2), and 5-LOX induced by ethanol or WRS. SK-MS10 also increased hexosamine, adherent mucus, and the expression of MUC5AC. Furthermore, SK-MS10 enhanced the mucosal expression of the CGRP gene and its serum levels.N(G)-methyl L-arginine (L-NMMA) or capsaicin desensitization reversed the SK-MS10-induced gastroprotection effect. These results suggest that SK-MS10 is a gastroprotective agent against acute gastric mucosal damage by suppressing proinflammatory cytokines, downregulating cPLA(2), 5-LOX, and increasing the synthesis of mucus. Furthermore, CGRP-NO pathway was found to play an important role in these gastroprotective effects of SK-MS10., (© Springer Science+Business Media, LLC 2009)
- Published
- 2009
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