1. Method for Ultrarapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen
- Author
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Yana Mishutkina, Sergey V. Leonov, Polina B. Volynchuk, Yaroslav Neskorodov, Alisher Touraev, Elena Marusich, Roman N. Chuprov-Netochin, Klaus Palme, and Yan A. Ivanenkov
- Subjects
0301 basic medicine ,Chemical compound ,Drug discovery ,Phenotypic screening ,Nicotiana tabacum ,fungi ,food and beverages ,Computational biology ,Biology ,medicine.disease_cause ,biology.organism_classification ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Pollen ,High-content screening ,medicine ,Arabidopsis thaliana ,Pollen tube - Abstract
Chemical genomics attracts considerable attention by offering crucial tools for plant biology to regulate plant growth and development. However, most chemical screens are time consuming and laborious, and require a high input of resources. Here we describe a broadly applicable method for the ultrarapid high-content phenotypic screening of small chemical compound libraries for new plant growth regulators. The assay is based on determination of pollen tube growth and can be completed in less than 8 h. Using this method, we identified novel inhibitors and modulators of plant growth and showed that compounds selected using a Nicotiana tabacum-based assay were biologically active in other plants, such as Arabidopsis thaliana.
- Published
- 2018