1. Manipulation and Measurement of AMPK Activity in Pancreatic Islets
- Author
-
Isabelle Leclerc, Aida Martinez-Sanchez, Guy A. Rutter, and Marie-Sophie Nguyen-Tu
- Subjects
0301 basic medicine ,endocrine system ,geography ,geography.geographical_feature_category ,endocrine system diseases ,030102 biochemistry & molecular biology ,Pancreatic islets ,AMPK ,Biology ,Islet ,Cell biology ,03 medical and health sciences ,030104 developmental biology ,medicine.anatomical_structure ,AMPK activity ,medicine ,Protein kinase A ,Insulin secretion ,Isolated islets ,Function (biology) - Abstract
The role of the energy sensor AMPK-activated protein kinase (AMPK) in the insulin-secreting β-cell remains unclear and a subject of intense research. With this chapter, we aim to provide a detailed description of the methods that our group routinely applies to the study of AMPK function in mouse and human pancreatic islets. Thus, we provide detailed protocols to isolate and/or culture mouse and human islets, to modulate and measure AMPK activity in isolated islets, and to evaluate its impact on islet function.
- Published
- 2018