1. Identification of Human Eosinophils in Whole Blood by Flow Cytometry
- Author
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Caroline Ethier, Francis Davoine, and Paige Lacy
- Subjects
education.field_of_study ,medicine.diagnostic_test ,CCR3 ,Population ,respiratory system ,Eosinophil ,Biology ,Flow cytometry ,Autofluorescence ,medicine.anatomical_structure ,Immunology ,medicine ,education ,Whole blood - Abstract
Identification of eosinophils in whole blood samples by flow cytometry is often problematic. There are usually only a low number and percentage of cells that may be detected, and it may be difficult to discriminate eosinophils from other granulocytes. Here, we propose a simple approach using the eosinophil's intrinsic autofluorescence properties, combined with detection of CCR3 expression, to reliably identify eosinophils in a mixed leukocyte population.
- Published
- 2014
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