1. Sample Preparation for Membrane Protein Structural Studies by Solid-State NMR
- Author
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Carole Gardiennet, Beat H. Meier, Britta Kunert, Anja Bo¨ckmann, Denis Lacabanne, Microbiologie moléculaire et biochimie structurale / Molecular Microbiology and Structural Biochemistry (MMSB), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Cristallographie, Résonance Magnétique et Modélisations (CRM2), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Physical Chemistry [ETH Zürich], Department of Chemistry and Applied Biosciences [ETH Zürich] (D-CHAB), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich)- Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), and Lacapère, Jean-Jacques
- Subjects
0301 basic medicine ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,biology ,Chemistry ,[SDV]Life Sciences [q-bio] ,Bacillus subtilis ,010402 general chemistry ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Sample quality ,03 medical and health sciences ,030104 developmental biology ,Solid-state nuclear magnetic resonance ,Membrane protein ,Structural biology ,Membrane reconstitution ,Biophysics ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Sample preparation ,ComputingMilieux_MISCELLANEOUS - Abstract
Conformational studies of membrane proteins remain a challenge in the field of structural biology, and in particular the investigation of the proteins in a native-like lipid environment. Solid-state NMR presents a valuable opportunity for this, and we present here three critical steps in the solid-state NMR sample preparation, i.e., membrane reconstitution of the protein in native lipids, rotor filling, and sample quality assessment, at the example of the Bacillus subtilis ATP-binding cassette transporter BmrA.
- Published
- 2017
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