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Since fresh cherry tomatoes have a high likelihood of spoilage during post-harvest transport and storage, assessing the fruit grade based on its firmness or predicting its potential storage time becomes necessary. The study proposes a method for non-destructive fruit firmness detection using a photometric stereo vision-based tactile sensor. The tactile sensor can measure the three-dimensional deformation of cherry tomatoes by mapping between pixel color and surface normal using a gradient prediction network. The summation of the deformation heights measured by a tactile sensor pressing on the cherry tomatoes can accurately describe fruit firmness, which was also verified by a comparative experiment with the Texture Analyzer. To create a model of firmness variation over time, a group of tomatoes of the same variety and origin were measured for firmness at various storage dates following harvest in subsequent experiments. An experiment was conducted to measure and predict the variation in the firmness of cherry tomatoes using a tactile sensor. The results indicate that the firmness of cherry tomatoes decreases linearly over time under similar conditions. The relative prediction errors were found to be mostly within ± 15% and ± 25% during 1 and 2 weeks of storage, respectively. All findings indicate that the proposed method can effectively measure the firmness of cherry tomatoes and accurately predict their short-term variations. [ABSTRACT FROM AUTHOR]

Mango (Mangifera indica L) is one of the most important tropical fruits worldwide; however, being a climacteric fruit, its postharvest deterioration is rapid, which implies that the presence of anthracnose caused by Colletotrichum gloesporioides and the long storage periods for its commercialization affect the shelf life of the fruit. The objective of this research was to evaluate the effect of three concentrations of a shellac-based wax and three concentrations of a biological coating (bioprotectant) made with Bacillus spp. and Streptomyces spp. as coatings for the preservation of physicochemical and microbiological quality parameters in Keitt mangoes stored for 35 days at 7 ºC ± 3. Mangoes with 0.5% bioprotectant and 30% wax showed an incidence of 40% up to day 35, which was significantly lower than that reported for the control group (80%). In the physicochemical parameters, the 30% wax preserved firmness, maintained a weight loss percentage of less than 10% during 35 days and presented the lowest pH values and the highest percentages of citric acid as of day 21 of storage, and the 0.5% bioprotectant in the variables of pH, total soluble solids and titratable acidity were similar to those reported in the control group; firmness was not affected during the days of storage. [ABSTRACT FROM AUTHOR]

The effect of Aloe vera (AV) gel coating was studied on antioxidant enzymes activities, oxidative stress, softening and associated quality attributes of persimmon fruits. The fruits were coated with 0 and 50% AV-gel coating and stored for 20 days at 20 ± 1 ºC. AV-gel coated fruits exhibited considerably less weight loss, hydrogen peroxide level, electrolyte leakage and malondialdehyde content. AV-gel coated fruits had significantly higher ascorbate peroxidase, peroxidase, superoxide dismutase and catalase activities. In addition, AV-gel coating suppressed pectin methylesterase, polygalacturonase and cellulase activities and showed higher ascorbic acid, DPPH scavenging antioxidants and phenolics, and lower sugars and carotenoids. To the best of our knowledge, these results are the first evidence that AV-gel coating modulates the activities of cell wall degrading enzymes to delay ripening in climacteric fruits. So, AV-gel coating prohibited the onset of senescence by activating enzymatic antioxidant system, accumulating bioactive compounds and suppressing cell wall degradation. [ABSTRACT FROM AUTHOR]

Background: Apricots originated from China, Central Asia and the Near East and arrived in Anatolia, and particularly in their second homeland of Malatya province in Turkey. Apricots are outstanding summer fruits, with their beautiful attractive color, delicious sweet taste, aroma and high vitamin and mineral content. Methods and results: In the current study, a total of 259 apricots genotypes from different geographical origins in Turkey were used. Significant variations were detected in fruit firmness (FF), fruit flesh color (FFC), flowering time (FT), and soluble solid content (SSC). A total of 11,532 SNPs based on DArT were developed and used in the analyses of population structure and association mapping (AM). According to the STRUCTURE (v.2.2) analysis, the apricot genotypes were divided into three groups. The mixed linear model with Q and K matrixes were used to detect the associations between the SNPs and four traits. A total of 131 SNPs were associated with FF, FFC and SSC. No SNP marker was detected associated with FT. Conclusion: The results demonstrated that AM had high potential of revealing the markers associated with economically important traits in apricot. The SNPs identified in the study can be used in future breeding programs for marker-assisted selection in apricot. [ABSTRACT FROM AUTHOR]

Fruit firmness is an important target of melon breeding, as it is associated with shelf life and economic value; however, the precise mechanism that determines fruit firmness during fruit ripening remains elusive. In the present study, one hundred forty-four F2 plants and F2–3 families derived from the high-firmness melon line M2–10 and the low-firmness melon line ZT091 were used to identify major quantitative trait loci by specific-locus amplified fragment sequencing with bulked segregant analysis. Simple sequence repeat (SSR) and cleaved amplified polymorphic sequence (CAPS) markers based on the resequencing of parental lines were also used to narrow the associated region to identify candidate genes. Two regions associated with fruit firmness were investigated, including a 4.87 Mb region on chr. 2 and a 28.7 Mb region on chr. 5 of the melon genome. SSR and CAPS markers were used to construct a genetic map of the associated regions: QTL ff5.1 was located between CmSSR13509 and CmSSR13423 and explained 38.44% of the observed variation, with an LOD threshold of 17.44; ff2.1 was located between CmSSR07709 and SNP22228 and explained 28.14% of the variation, with an LOD threshold of 3.8, and this region included 106 Kb and 10 candidate genes. Quantitative real-time PCR was performed to investigated the investigate candidate gene expression at 15, 20 and 25 days after pollination in the parental lines, and significant expression levels were detected for most of the genes, including four genes of unknown function and MELO3C017519, MELO3C017520, MELO3C017522, MELO3C029506, and MELO3C029520. These results revealed a new QTL, ff2.1, for melon fruit firmness-related gene identification. [ABSTRACT FROM AUTHOR]

Fruit firmness is an important trait of melons due to its effect on fresh fruit consumption, storage, and transport. However, information on the expression of genes influencing the fruit firmness of 'Baogua' (BG) melon (Cucumis melo ssp. agrestis Jeffrey) remains rare. This study aimed to identify the key genes associated with the firmness of BG fruit sampled at 14 and 28 days after pollination (dap) via transcriptome sequencing. A total of 1113 up-regulated and 2224 down-regulated differentially expressed genes (DEGs) were identified. The main Gene Ontology terms assigned to the DEGs were phosphotransferase activity, alcohol group as acceptor, protein phosphorylation, and protein kinase activity. The enriched KEGG pathways involving the DEGs were starch and sucrose metabolism, diterpenoid biosynthesis, plant hormone signal transduction, and MAPK signaling pathway–plant. In addition, qRT-PCR verified that four GAL genes, namely, CmGAL1–4, were differentially expressed at 0, 7, 14, 21, and 28 dap. Our data revealed that CmGAL1 expression was highest at 21 dap. However, the expression levels of CmGAL2–4 were highest at 14 dap. The sequence of CmGAL1 was similar to the sequences of homologs from melon and cucumber. Subcellular localization analysis revealed CmGAL1 was located in the cell membrane and cytoplasm. Our findings implied that fruit development at 14 dap, which is a key time-point, varies considerably from fruit development at 28 dap. Our present study provides new information on the genes associated with BG fruit firmness and help improve the storage and transport of BG fruit prior to processing. [ABSTRACT FROM AUTHOR]

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In this research, the effects of pre-harvest 0.25% iron treatments on yield, some fruit quality parameters, biochemical properties and micronutrient content of 'Santa Maria', 'Deveci' and 'Akça' pear cultivars were investigated. Effects of iron treatments on yield were found to be significant. While fruit firmness decreased with iron treatments in 'Santa Maria' and 'Akça' pears, the value increased in 'Deveci' cultivar. Besides, total phenolic compounds and antioxidant activity also decreased with iron treatments. Effects of iron treatments on individual phenolics were not found to be significant. It was concluded that foliar iron treatments had positive effects in all cultivars. [ABSTRACT FROM AUTHOR]

The effects of pre-harvest applications of calcium nitrate at different concentrations were investigated on storability and quality attributes of plum fruits under low temperature storage. Uniform and healthy trees of plum cv. Satluj Purple were sprayed with calcium nitrate (1.0%, 1.5% and 2.0%) in 2nd and 3rd week of April and control trees were water sprayed only. Fruits from treated and control trees were harvested at colour break stage, and then stored at low temperature conditions (0-1 °C and 90-95% RH) for 35 days. Evaluation of physico-chemical parameters and enzymatic activities were made on the day of harvest and after 7, 14, 21, 28 and 35 days of storage. The colour of the fruits with respect to a* and b* values (green-red and blue-yellow spectrum, respectively) improved progressively up to 35 days of storage, whereas, fruit firmness decreased. Total sugars and pectin methylesterase (PME) and cellulase activities increased up to 28 days of storage before showing a declining trend. Two superimposed sprays of calcium nitrate (2.0%) enhanced the storage life of plum fruits up to 28 days by reducing physiological loss in weight (PLW), PME and cellulase activities, delaying development of fruit colour & anthocyanin and retaining sensory quality, firmness and total sugars in plum fruits. [ABSTRACT FROM AUTHOR]

The objective of the present work is to see the effect of different chemical treatments on pectin methylesterase (PME) activity and softening of the fruits. Purple plums ( cv. Satluj) fruits were harvested at colour break stage and treated for 5- minutes in aqueous solutions of salicylic acid at 138, 276 and 414 ppm, Ascorbic acid at 10,000, 20,000 and 30,000 ppm, and Gibberelic acid at 20, 40 and 60 ppm. For control the fruits were dipped in distilled water. Treated fruits were stored at low temperature conditions for 40 days. Physico chemical characteristics and PME activity were determined at 10 day interval. The colour of the fruits improved with respect to a* and b* values progressively throughout the storage, whereas, fruit firmness decreased. Total sugars and PME activity increased before showing a declining trend. Pre-storage application of salicylic acid (414 ppm) significantly delayed colour development. Fruits treated with salicylic acid (414 ppm) retained highest firmness, total sugars and PME activity at the end of storage. [ABSTRACT FROM AUTHOR]

Fruit firmness and weight are among the most important fruit quality traits in fruit species. Understanding the control of fruit firmness and weight is essential for the development of domestication research approaches and for the implementation of new breeding strategies. A forward genetic study for these traits was performed using two F1 sweet cherry ( Prunus avium) progenies derived from modern cultivars. Quantitative trait locus (QTL) analysis allowed the identification of genomic regions accounting for most of the phenotypic variation in both families. In addition, screening the Prunus persica genome v1.0 permitted the identification of putative candidate genes underlying the QTL with the major effect for fruit weight (LG5) and the one for firmness (LG6). A colocalization of QTLs and candidate genes was found in peach, apple, and tomato. These results give new insights of the interaction between fruit firmness and fruit weight and provide new cues for the identification of genes implicated in the control of these traits. The colocalization of genomic regions between progenies issued from modern cultivars and from modern cultivars × wild individuals suggests the absence of allele fixation within genes controlling fruit firmness and size, two traits potentially involved in domestication/diversification in sweet cherry. [ABSTRACT FROM AUTHOR]

Fatty acid compositions of peach ( Prunus persica L. Batsch) mesocarp tissues from 'Kawanakajima Hakuto' and its firm-fleshed mutant 'Shuangjiuhong' were examined by gas chromatography during the developmental stages from 20 days before to 20 days after fruit ripening. Fruits were harvested at 4-day intervals from July to September. The predominant fatty acids were linoleic, palmitic and linolenic acids with 27.66-48.93 %, 23.59-31.65 %, and 12.08-28.35 % in 'Shuangjiuhong', and 32.64-42.79 %, 23.53-28.95 %, 16.14-39.15 % in 'Kawanakajima Hakuto', respectively. Saturated fatty acids (palmitic and stearic acids) remained relatively constant throughout the ripeness period. On the contrast, from 15 days before ripening, notable decline in oleic acid and increase of linoleic and linolenic acids were observed in both cultivars. In addition, from 10 days before ripening, much lower levels of oleic and linolenic acids and higher proportion of linoleic acid were observed in 'Shuangjiuhong' than those found in 'Kawanakajima Hakuto'. And notably higher SFA level, lower levels of UFA and IUFA in the firm-fleshed peach were investigated during those stages. Correlation analysis showed that oleic acid and SFA had very significantly positive, whereas linolenic acid, UFA and IUFA had significantly negative correlation with fruit firmness. These results above suggest that lower levels of oleic and linolenic acids, UFA and IUFA, and higher linoleic acid and SFA content represent fruits with firmer flesh and help to retain the fruit texture. [ABSTRACT FROM AUTHOR]

Strawberry is a soft fruit with a short postharvest shelf-life. The loss of fruit firmness during ripening is mainly due to the disassembly of parenchyma cell walls mediated by the expression of genes encoding enzymes acting on pectins, such as pectate lyase, or hemicellulose, e.g. endo-β-1,4-glucanase. To determine if the simultaneous down-regulation of FaplC and FaEG3 genes, encoding a pectate lyase and a endo-β-1,4-glucanase, respectively, exerted an additive effect on strawberry softening, transgenic plants expressing tandem antisense sequences of both genes under the control of the constitutive promoter CaMV35S were generated. Fifteen independent transgenic lines were obtained and fruit yields and several quality parameters of transgenic ripe fruit were recorded during two consecutive years. Fruit yield was reduced in most of the lines, especially in the first evaluation period, and five out of 15 lines (33 %) did not set fruit. The expression of FaplC and FaEG3 genes was measured in ripe fruits from six selected lines showing the highest fruit yields. All selected lines showed a high level of FaplC gene silencing, ranging from 97 to 71 %; however, FaEG3 gene expression was only significantly down-regulated in two lines. Fruit colour and soluble solids contents were similar in control and transgenic ripe fruits, while fruit weight was slightly lower than control in some of the lines. In all lines, transgenic fruits were significantly firmer than control, with an increase in firmness ranging from 19 to 32 %. The reduction of fruit softening in transgenic fruits was not correlated with the suppression of FaEG3 gene expression, and lines with the highest simultaneous down-regulation of FaplC and FaEG3 showed similar fruit firmness to lines where only FaplC was suppressed. These results indicate that pectate lyase and endo-β-1,4-glucanase do not act in an additive or synergistic way during strawberry softening, and question the role of glucanases in this process. [ABSTRACT FROM AUTHOR]

The effects of global warming and CO level were investigated on tree growth and fruit characteristics of 'Niitaka' pear. The treatments consisted of ambient temperature and 390 μL·L CO (control group), ambient temperature + 4°C and 390 μL·L CO (elevated temperature group), ambient temperature and 700 μL·L CO (elevated CO group), and ambient temperature + 4°C and 700 μL·L CO (elevated climate group). Fruit weight was the highest in elevated CO group (543.0 g), but lowest in elevated temperature group (394.3 g). Flesh firmness at harvest was the highest in elevated temperature group. For fruit skin color, Hunter L* and b* values were not significantly different among treatments, while Hunter a* value was higher on those treated with higher temperature and CO concentration. Photosynthetic rate in July and August was high in plants under elevated CO concentration. In contrast, the elevated temperature and elevated climate groups showed relatively high photosynthetic rate in early July, then became far lower than those of the control and the elevated CO group in late August. Rapid increase in fruit diameter was observed on fruits grown under elevated climate, then slowed down after the middle fruit growth stage. Soluble solids content increased at harvest time in groups with elevated CO. [ABSTRACT FROM AUTHOR]

Fruit firmness constitutes an important textural property and is one of the key parameters for estimating ripening and shelf life, which has a major impact on commercialization. In order to decipher the mechanisms related to firmness of apples ( Malus × domestica Borkh.), two-dimensional gel electrophoresis (2-DE) was used to compare the total proteome of high and low firmness phenotypes from apple hypanthia of a 'Golden Delicious' × 'Dietrich' population. A total of 36 differentially regulated protein spots were positively identified by matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and then validated against the Malus expressed sequence tags (EST) database. The findings of this study indicated a lower expression of ethylene biosynthesis related proteins in the high firmness phenotype, which could be linked to the slowing down of the ripening and softening processes. The reduced accumulation of proteins involved in ethylene biosynthesis juxtaposed to the upregulation of a transposase and a GTP-binding protein in the high firmness phenotype. The results also showed higher expression of cytoskeleton proteins in the high firmness phenotype compared to the low firmness phenotype, which play a role in maintaining cell structure and possibly fruit integrity. Finally, a number of proteins involved in detoxification and defense were expressed in fruit hypanthium. This proteomic study provides a contribution towards a better understanding of regulatory networks involved in fruit hypanthium firmness and/or softening, which could be instrumental in the development of improved fruit quality. [ABSTRACT FROM AUTHOR]

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Fruit ethylene production genotypes for Md-ACS1 and Md-ACO1 were determined for 60 apple cultivars and 35 advanced breeding selections. Two alleles for each gene are commonly found in cultivated apple. Earlier studies showed that genotypes homozygous for the ACS1-2 allele produce less ethylene and have firmer fruit than ACS1-1/2 and ACS1-1/1 genotypes. ACO1 plays a minor role compared to ACS1, with homozygous ACO1-1 having lower ethylene production. In this study, ACS1-2 and ACO1-1 homozygotes had firmer fruit at harvest and after 60 days of 0–1°C cold storage compared to other genotypes. These genotypes, ACS1-2/2 and ACO1-1/1, were observed for the following 8 of 95 cultivars/selections: “Delblush”, “Fuji”, “Pacific Beauty”, “Sabina” and four breeding selections. Cultivars/selections that were homozygous ACS1-2 but not ACO1-1 were: “Ambrosia”, “Aurora Golden Gala”, “CrimsonCrisp”, “Gala”, “GoldRush”, “Huaguan”, “Pacific Rose, “Pacific Queen”, “Pinova”, “Sansa”, “Sonja”, “Sundance”, “Zestar”, and 17 breeding selections. Cultivars with the heterozygous ACS1-1/2 genotype were “Arlet”, “Braeburn”, “Cameo”, “Delicious”, “Delorgue”, “Empire”, “Enterprise”, “Ginger Gold”, “Golden Delicious”, “Granny Smith”, “Honeycrisp”, “Orin”, “Pink Lady”, “Silken”, “Suncrisp”, “Sundowner”, “Sunrise” and 11 breeding selections. No cultivars were detected homozygous for both ACS1-1 and ACO1-1, or for both ACS1-2 and ACO1-2. This study is the first large-scale allelic genotyping of both ethylene synthesis genes for a comprehensive set of apple breeding parents used in an ongoing breeding project. The data reported here are important for informative selection of parent combinations and marker-assisted selection of progeny for breeding low ethylene-producing apple cultivars for better storability and improved consumer acceptance. [ABSTRACT FROM AUTHOR]

Copyright of Erwerbs-Obstbau is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Erwerbs-Obstbau is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Erwerbs-Obstbau is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Erwerbs-Obstbau is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Erwerbs-Obstbau is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Field experiments were carried out at the Horticultural Research Institute, and in five commercial apple orchards, located in southwest Finland including the Aland Islands during 1994 and 1995. The experimental cultivars were Melba, Raike, Red Atlas, Akerö, Aroma, and Lobo. The treatments were untreated control and preharvest calcium chloride (CaCl2) sprays at Ca 2.0 g/l. Fruit samples were stored for two to six months at 2-4 degrees C and 85-95% RH. The percentage of the incidence of physiological disorders of stored apples was scored. Fruit macronutrients, firmness, diameter and juice titratable acidity and percentage of soluble solids were determined. Only a few nutrient effects in the flesh of the apple cultivars were a result of CaCl2 sprayings. Preharvest CaCl2 sprays increased fruit firmness and the titratable acidity but decreased soluble solids, soluble solids/titratable acidity ratio, and the incidence of physiological storage disorders of some cultivars. When looking at the mean of all cultivars, CaCl2 sprayings increased titratable acidity and decreased soluble solids/titratable acidity ratio after four months of storage during 1995 and reduced the incidence of physiological disorders after three months of storage during 1994 and after four months of storage in 1995. [ABSTRACT FROM AUTHOR]