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Cancer stemness represents a major source of development and progression of colorectal cancer (CRC). c-Met critically contributes to CRC stemness, but how c-Met is activated in CRC remains elusive. We previously identified the lipolytic factor ABHD5 as an important tumour suppressor gene in CRC. Here, we show that loss of ABHD5 promotes c-Met activation to sustain CRC stemness in a non-canonical manner. Mechanistically, we demonstrate that ABHD5 interacts in the cytoplasm with the core subunit of the SET1A methyltransferase complex, DPY30, thereby inhibiting the nuclear translocation of DPY30 and activity of SET1A. In the absence of ABHD5, DPY30 translocates to the nucleus and supports SET1A-mediated methylation of YAP and histone H3, which sequesters YAP in the nucleus and increases chromatin accessibility to synergistically promote YAP-induced transcription of c-Met, thus promoting the stemness of CRC cells. This study reveals a novel role of ABHD5 in regulating histone/non-histone methylation and CRC stemness. This study reveals an unrecognized role of ABHD5 in regulating colon cancer stemness via controlling YAP methylation and nuclear localization, further explaining the molecular mechanism through which ABHD5 functions as a tumour suppressor gene in colon cancer. [ABSTRACT FROM AUTHOR]

These authors contributed equally: Yan Gu, Yanrong Chen, Lai Wei, Shuang Wu. Correction to: I Nature Communications i https://doi.org/10.1038/s41467-021-26967-5, published online 18 November 2021 This Article contains an error in Figure 2j. [Extracted from the article]

An electrochemical sensor is developed in this work based on the new perovskite-type nanomaterial LaNiTiO-FeO for sensitive determination of o-phenylenediamine (OPD). As-synthesized materials and the surface of as-fabricated electrochemical sensor are characterized by X-ray diffraction, atomic force microscope, and electrochemical impedance spectroscopy, respectively. The results of characterizations depict that the sample is of nanoscaled complex oxides consisting of perovskite structure and spinel structure, and has good conductive properties. The construction and experimental conditions of the electrochemical sensor are also optimized. The electrochemical properties of OPD at glassy carbon electrode modified with LaNiTiO-FeO are investigated in alkaline solution (NaOH). The new electrochemical sensor exhibits high electrocatalytic activity and stability in NaOH, and a promotion of electrochemical oxidation of OPD at low potentials can be obviously observed. A wide linear range is obtained from 1.0 × 10 to 7.0 × 10 M with a relative low detection limit of 0.15 μM (S/N = 3) under optimal conditions. Furthermore, the sensor exhibits reliable results for the determination of OPD in commercial samples. [ABSTRACT FROM AUTHOR]

Describes the design and application of 60mer oligonucleotide microarray in SARS coronavirus detection. Diagnostic capability of the microarray; Results indicating that the genome of SARS coronavirus can be detected in parallel by the 60mer oligonucleotide microarray which can improve the positive ratio of the diagnosis.