Search

Key message: Six MnSOD genes were isolated from five Miscanthus species. MgMnSOD1 functions in mitochondria and MgMnSOD1 seems to be the main MnSOD gene involved in stress response of M. × giganteus. Abstract: Miscanthus × giganteus is a promising biomass energy crop with advantages of vigorous growth, high yield, low fertilizer and pesticide inputs. However, poor overwinter ability limits its widespread cultivation. Moreover, narrow genetic base may increase the risk of susceptibility to diseases and pests. Manganese superoxide dismutase (MnSOD), an important antioxidant enzyme involved in stress tolerance is able to protect plant cells from accumulated reactive oxygen species by converting superoxide to peroxide and oxygen. In many plants, overexpression of MnSOD has shown the ability to enhance the resistance to various stresses. This article describes the studies performed in an attempt to elucidate the molecular and enzymatic properties of MnSODs in M. × giganteus. MnSOD genes from M. × giganteus ( MgMnSOD1, MgMnSOD2), M. lutarioriparia ( MlMnSOD), M. sacchariflora ( MsaMnSOD), M. sinensis ( MsiMnSOD), and M. floridulus ( MfMnSOD) were cloned and sequenced. The sequence analysis and expression patterns of MgMnSOD1 and MgMnSOD2 suggest that they were orthologous genes which were inherited from the two parents, M. sacchariflora and M. sinensis, respectively. In addition, MgMnSOD1 is predicted to be the main MnSOD gene involved in stress response of M. × giganteus. The activity of purified recombinant MgMnSOD1 was 1854.79 ± 39.98 U mg (mean ± SD). Further enzymatic assays revealed that the protein exhibited an outstanding thermal stability. MgMnSOD1 is predicted to be targeted to mitochondria and involved in removing the superoxide radical generated by respiration. The presence and sequences of other SOD isozymes transcripts were also investigated in this study. [ABSTRACT FROM AUTHOR]

The trapping stiffness and width are two important parameters to characterize a counter-propagating dual-beam optical trap. We present two types of asymmetric counter-propagating dual-beam optical trap with the different numerical aperture (NA) and trapping power to eliminate the multi-equilibrium positions when two foci of the optical trap are not coincided. Meanwhile, the asymmetric dual-beam trap with the different NA enhances the axial trapping width and stiffness over five and three times, respectively, higher than the standard dual-beam trap with the higher and same average NA. Besides, it increases the transverse trapping stiffness when two foci are not coincided. The asymmetric dual-beam optical trap will benefit the future applications for the study of precision measurement, basic physics and biomaterials. [ABSTRACT FROM AUTHOR]