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In this note, we show a sharp lower bound of min { ∑ i = 1 k d G (u i) : u 1 u 2 ... u k is a path of (2-)connected G on its order such that (k-1)-iterated line graphs Lk−1(G) are hamiltonian. [ABSTRACT FROM AUTHOR]

The acidified single-walled carbon nanotubes (SWCNTs) were self-assembled on graphene oxide (GO) and then ultrasonically dispersed in a copolymer, Nafion solution, to form a GO/SWCNTs-Nafion polymer nanocomposite, which was employed to modify glassy carbon electrode (GCE). The surface morphological characteristics of different modified electrodes including bare GCE, GO-Nafion/GCE, and GO/SWCNTs-Nafion/GCE were imaged by scanning electron microscopy. For comparison, the differential pulse voltammetry and cyclic voltammetry behaviors were investigated, showing that the GO/SWCNTs-Nafion polymer composite has strong enhancement effect towards oxidation of clenbuterol (CLB). And the corresponding mechanism has been well discussed. During the reaction process, the anilino group of CLB molecule ( 1) was firstly oxidized to form a radical cation ( 2), exhibiting a characteristic oxidation peak (I) at 0.95 V, then two radical cations reacting via head-to-head coupling to form a diphenylamine intermediate ( 3), which was transformed into a CLB dimmer ( 4) through an azo bond by intramolecular electrons transferring under low potential, exhibiting a pair of reversible oxidation peak (II) and reduction peak (III). Under the optimum conditions, the composite modified electrode showed linear response to CLB in a concentration range of 1.0 × 10~6.0 × 10 mol/L with a detection limit of 6.0 × 10 mol/L. The modified electrode possessed good selectivity, reproducibility, and stability. In comparison with two routine analytical methods like ELISA kit and high-performance liquid chromatography (HPLC), the electrode can be successfully applied to determination of content of CLB in pig meat and pig liver samples with a recovery rate of 96.4~104.2%, suggesting a promising application in food security field. [ABSTRACT FROM AUTHOR]

Plasma-modified multiwalled carbon nanotubes (MWNTs) were used as a support to immobilize lipase. The effects of vacuum plasma treatment power, vacuum plasma treatment time, immobilization temperature, immobilization time, and initial protein concentration of the lipase on the amount of lipase immobilized and on the subsequent activity of the immobilized lipase were investigated. The results showed that the adsorption capacity of the plasma-modified MWNTs could reach 0.15 g/g and that the maximal enzyme activity of the immobilized lipase was 520U/g under optimized conditions. Fourier transform infrared (FTIR) analysis and transmission electron microscopy (TEM) were used to characterize the properties of the plasma-modified MWNTs and plasma-modified MWNTs-lipase, and the results showed that the lipase was successfully immobilized on the plasma-modified MWNTs. Also, the MWNTs-lipase produced an esterification rate of approximately 47% in the synthesis of polyethylene glycol (PEG)-aliphatic esters. [ABSTRACT FROM AUTHOR]

Ground observation is able to obtain highly accurate data with high temporal resolution at observation points, but these observation points are too sparsely to satisfy the application requirements at regional scale. Satellite remote sensing can frequently supply spatially continuous information on earth surface, which is impossible from ground-based investigations, but remote sensing description is not able to directly obtain process parameters. In fact, in terms of fundamental theorem of surfaces, a surface is uniquely defined by the first fundamental coefficients, about the details of the surface observed when we stay on the surface, and the second fundamental coefficients, the change of the surface observed from outside the surface. A method for high accuracy surface modeling (HASM) has been developed initiatively to find solutions for error problem and slow-speed problem of earth surface modeling since 1986. HASM takes global approximate information (e.g., remote sensing images or model simulation results) as its driving field and local accurate information (e.g., ground observation data and/or sampling data) as its optimum control constraints. Its output satisfies the iteration stopping criterion which is determined by application requirement for accuracy. This paper reviews problems to be solved in every development stage and applications of HASM. [ABSTRACT FROM AUTHOR]

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We have found that a spatial interpolation of mean annual temperature (MAT) in China can be accomplished using a global ordinary least squares regression model since the relationship between temperature and its environmental determinants is constant. Therefore the estimation of MAT does not very across space and thus exhibits spatial stationarity. The interpolation of mean annual precipitation (MAP), however, is more complex and changes spatially as a function of topographic variation. Therefore, MAP shows spatial non-stationarity and must be estimated with a geographically weighted regression. A statistical transfer function (STF) of MAT was formulated using minimized residuals output from a high accuracy and high speed method for surface modeling (HASM) with an ordinary least squares (OLS) linear equation that uses latitude and elevation as independent variables, abbreviated as HASM-OLS. The STF of MAP under a BOX-COX transformation is derived as a combination of minimized residuals output by HASM with a geographically weighted regression (GWR) using latitude, longitude, elevation, impact coefficient of aspect and sky view factor as independent variables, abbreviated as HASM-GWR-BC. In terms of HASM-OLS and HASM-GWR-BC, MAT had an increasing trend since the 1960s in China, with an especially accelerated increasing trend since 1980. Overall, our data show that MAT has increased by 1.44 °C since the 1960s. The warming rates increase from the south to north in China, except in the Qinghai-Xizang plateau. Specifically, the 2,100 °C · d contour line of annual accumulated temperature (AAT) of ≥10 °C shifted northwestward 255 km in the Heilongjiang province since the 1960s. MAP in Qinghai-Xizang plateau and in arid region had a continuously increasing trend. In the other 7 regions of China, MAP shows both increasing and decreasing trends. On average, China became wetter from the 1960s to the 1990s, but drier from the 1990s to 2000s. The Qinghai-Xizang Plateau and Northern China experienced more climatic extremes than Southern China since the 1960s. [ABSTRACT FROM AUTHOR]

Bovine lactoferrampin (LFA) and bovine lactoferricin (LFC) are two antimicrobial peptides located in the N domain of bovine lactoferrin. The bactericidal activity of the fused peptide LFA-LFC is stronger than that of either LFA or LFC. The high cost of peptide production from either native digestion or chemical synthesis limits the clinical application of antimicrobial peptides. The expression of recombinant peptides in yeast may be an effective alternative. In the current study, the expression, purification, and antibacterial activity of LFA-LFC using the Pichia pastoris expression system are reported. The linearized expression vector pPICZaA-LFA-LFC was transformed into P. pastoris KM71 by electroporation, and positive colonies harboring the target genes were screened out and used for fermentation. The recombinant LFA-LFC peptide was purified via two-step column chromatography and identified by tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The results indicate that P. pastoris is a suitable system for secreting LFA-LFC. The fermentation supernate and the purified LFA-LFC show high antimicrobial activities. The current study is the first to report on the expression and purification of LFA-LFC in P. pastoris and may have potential practical applications in microbial peptide production. [ABSTRACT FROM AUTHOR]