1. Local administration of TGF-beta1 to reinforce the anterior abdominal wall in a rat model of incisional hernia.
- Author
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Korenkov, M, Yuecel, N, Koebke, J, Schierholz, J, Morsczeck, Ch, Tasci, I, Neugebauer, E A M, and Nagelschmidt, M
- Subjects
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ABDOMINAL surgery , *HERNIA surgery , *ABDOMINAL physiology , *ANIMAL experimentation , *COLLAGEN , *GROWTH factors , *INTRAMUSCULAR injections , *POLYENES , *POLYESTERS , *RATS , *RECOMBINANT proteins , *SUTURES , *WOUND healing , *TENSILE strength , *SURGICAL meshes - Abstract
The purpose of this study was to investigate different forms of the local application of TGF-beta(1) for augmentation of the anterior abdominal wall in an appropriate model of an incisional hernia. Sixty male Sprague-Dawley rats were divided into six groups. Artificial defects of the anterior abdominal wall were closed with one of the following methods: running Prolene suture, Vicryl mesh, prolene suture followed by an intramuscular injection of 1 mug TGF-beta(1), Vicryl mesh coated with 1 mug TGF-beta(1), and prolene suture coated with 1 mug TGF-beta(1). A control group did not receive any defect and treatment. Six weeks after operation, tensile strength, collagen content, gene expression of collagen I and III, blood vessels, and thickness of collagen fibres were evaluated. Tensile strength was strongest in the controls (14.2 (10.5-18 N)). There was no increase in tensile strength due to the administration of TGF-beta(1). On the contrary, bolus injection of the growth factor resulted in a significantly decreased strength of the wound tissue when compared to the groups 1, 4, 5, and 6 (9.1 (4.2-9.1 N)). These results correlated with the gene expression of collagen I and III. Local application of TGF-beta(1) did not augment the strength of the abdominal wall after 6 weeks. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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