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A general program SAWBEH is proposed for the kinetic data evaluation of enzyme-catalysed hydrolysis by using the frequency shift response of an SAW sensor device. The Marquardt-Fletcher algorithm was employed as the minimum basis. The program was validated theoretically and compared with normal linear analysis methods by using synthetic data added with noise at different levels. The standard deviations of results obtained by the latter are about 100 times those obtained by the former. The system trypsin/benzoyl-L-arginine ethyl ester (BAEE) as a test model was studied at pH 7.6–7.8 and 32 °C. The Michaelis constant was estimated to be 6.34 × 10−5 M. [ABSTRACT FROM AUTHOR]